Application of quercetin and aconitine in combination for preparing medicine for treating cervical cancer and medicine for treating cervical cancer
A technology of aconitine and quercetin, applied in the field of medicine, can solve unclear problems
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[0018] Example 1 The combination of quercetin and aconitine inhibits the proliferation of HeLa cells
[0019] 1. MTT method to detect cell proliferation
[0020] The cell proliferation was detected by tetramethylazolium salt colorimetric method (MTT method). Cells in the logarithmic growth phase were inoculated into 96-well culture plates, 100 μL per well, 37 °C, 5% CO 2 cultured in an incubator. After 24h, different concentrations of drugs were added. The final concentrations of quercetin were 1.56, 3.12, 6.25, 12.5, 25, 50, and 100 μg / mL; the final concentrations of aconitine were 1.56, 3.12, 6.25, 12.5, 25, 50, and 100 μg / mL; The concentration ratio of the two drugs is 1:1, and the final concentrations of the two drugs are 3.12, 6.25, 12.5, 25, 50, 100, and 200 μg / mL. After culturing for 48 hours, discard the culture medium, add 10 μL of MTT (5 mg / mL) to each well, and continue culturing for 4 hours; discard the supernatant, add 100 μL of dimethyl sulfoxide to each well...
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