Protein-polyphenol composite microsphere as well as preparation method and application thereof

A technology of composite microspheres and polyphenolic compounds, which can be used in pharmaceutical combinations, pharmaceutical formulations, medical preparations with inactive ingredients, etc. Insufficient and other problems, to achieve good long-term dispersion stability, enhance anti-tumor effect, and avoid the effect of energy-consuming equipment

Active Publication Date: 2018-06-05
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current method of preparing protein-polyphenol composites still has great limitations: (1) The dispersion and stability of nanomaterials are not good, and the particle size range cannot be adjusted and the adjustment range is narrow. Large-scale particle size control from nanometer to micron; (2) The loading capacity of polyphenols is very low and most of them do not exc

Method used

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  • Protein-polyphenol composite microsphere as well as preparation method and application thereof
  • Protein-polyphenol composite microsphere as well as preparation method and application thereof
  • Protein-polyphenol composite microsphere as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] The protein extract used in this example is human hair keratin, the polyphenolic compound is tea polyphenol (Sun Lubao, Wuxi, Jiangsu, Lot 209271), and the aldehyde compound is formaldehyde.

[0048] The preparation process of protein-polyphenol composite microspheres prepared in this embodiment is as follows:

[0049] (1) Add 500mg, 100mg, 50mg, and 10mg of human hair keratin into four beakers containing 50ml of aqueous solution, and completely dissolve the human hair keratin at room temperature under magnetic stirring to prepare a concentration of 10mg / ml, 2mg / ml, 1mg / ml, 0.2mg / ml human hair keratin extract aqueous solution;

[0050] (2) Add 2500mg, 1000mg, 500mg, 100mg, and 10mg of tea polyphenols into 5 beakers filled with 50ml aqueous solution respectively, and completely dissolve the tea polyphenols at room temperature under magnetic stirring to prepare a concentration of 50mg / ml , 20mg / ml, 10mg / ml, 2mg / ml, 0.2mg / ml tea polyphenol aqueous solution;

[0051] (3)...

Embodiment 2

[0056] The protein extract used in this example is wool keratin, the polyphenolic compound is tea polyphenol (Sun Lubao, Wuxi, Jiangsu, Lot 209271), and the aldehyde compound is formaldehyde.

[0057] The preparation process of protein-polyphenol composite microspheres prepared in this embodiment is as follows:

[0058] (1) Add 500mg, 100mg, 50mg, and 10mg of wool keratin to 4 beakers containing 50ml aqueous solution respectively, and completely dissolve the wool keratin at room temperature under magnetic stirring, and prepare a concentration of 10mg / ml, 2mg / ml, 1mg / ml, 0.2mg / ml wool keratin extract aqueous solution;

[0059] (2) Add 2500mg, 1000mg, 500mg, 100mg, and 10mg of tea polyphenols into 5 beakers filled with 50ml aqueous solution respectively, and completely dissolve the tea polyphenols at room temperature under magnetic stirring to prepare a concentration of 50mg / ml , 20mg / ml, 10mg / ml, 2mg / ml, 0.2mg / ml tea polyphenol aqueous solution;

[0060] (3) 500 μl of formald...

Embodiment 3

[0065] The protein extract used in this example is feather keratin, the polyphenolic compound is tea polyphenol (Sun Lubao, Wuxi, Jiangsu, Lot 209271), and the aldehyde compound is formaldehyde.

[0066] The preparation process of protein-polyphenol composite microspheres prepared in this embodiment is as follows:

[0067] (1) 500mg, 100mg, 50mg, and 10mg of feather keratin were added to 4 beakers containing 50ml aqueous solution respectively, and the feather keratin was completely dissolved at room temperature under magnetic stirring to prepare a concentration of 10mg / ml, 2mg / ml, 1mg / ml, 0.2mg / ml aqueous solution of feather keratin extract;

[0068] (2) Add 2500mg, 1000mg, 500mg, 100mg, and 10mg of tea polyphenols into 5 beakers filled with 50ml aqueous solution respectively, and completely dissolve the tea polyphenols at room temperature under magnetic stirring to prepare a concentration of 50mg / ml , 20mg / ml, 10mg / ml, 2mg / ml, 0.2mg / ml tea polyphenol aqueous solution;

[0069...

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Abstract

The invention discloses a protein-polyphenol composite microsphere as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing a protein extract aqueous solution and a polyphenol compound aqueous solution, initiating crosslinking reaction by a compound with free aldehyde, and regulating and controlling through reaction between the protein extract and the polyphenol compound to obtain the protein-polyphenol composite microsphere with wide grain diameter range distribution (10 mum to 10 nm) and regulable and controllable grain size. The protein-polyphenol composite microsphere obtained through preparation can transmit the polyphenol compound, the protein extract and the aldehyde compound which serve as the reaction raw materials, alsocan transmit a medicine interacted with the protein and the polyphenol, and can effectively enhance the antitumor effect of the medicine when serving as a tumor medicine carrier.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and relates to a protein-polyphenol composite micro / nano composite material and a preparation method and application thereof. Background technique [0002] Polyphenolic compounds are a class of substances with polyphenolic structures that widely exist in a variety of plants and are abundant in reserves. For example, the content of tea polyphenols in green tea can reach 40% of the dry weight of tea leaves. Tea polyphenols have excellent biological properties such as antioxidant, anticancer, antibacterial and anti-inflammatory, and antiviral, and are widely used in food, daily chemical, medical and health fields. However, the metabolism rate of polyphenols in the body is too fast, resulting in low bioavailability, which affects the utilization of polyphenol resources. [0003] The use of polyphenols and proteins to prepare nanomaterials for anti-tumor research has shown good anti-tumor effect...

Claims

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Application Information

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IPC IPC(8): A61K47/64A61K47/69A61K47/42A61K45/00A61K45/06A61K31/353A61K31/11A61K31/7024A61P35/00
CPCA61K31/11A61K31/353A61K31/7024A61K45/00A61K45/06A61K47/42A61K2300/00
Inventor 李旭东易增陈光灿孙哲马晓敏苏文崔新星陈翔宇
Owner SICHUAN UNIV
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