A method for detecting hypochlorite by near-infrared

A near-infrared detection and hypochlorite technology, which is used in measurement devices, material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of fluorescence signal interference, strong visible light scattering, etc., and achieve broad application prospects, low cost, and high sensitivity. and selective effects

Active Publication Date: 2021-01-22
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] However, most of the fluorescent probes reported in the literature for the detection of hypochlorite have maximum absorption and emission wavelengths in the visible region (400–600 nm).
In the visible light region, some endogenous fluorophores in the environment and in organisms and tissues produce "autofluorescence". At the same time, biological tissues strongly scatter visible light, which seriously interferes with the fluorescence signals of exogenous fluorescent probes.

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  • A method for detecting hypochlorite by near-infrared
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  • A method for detecting hypochlorite by near-infrared

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Embodiment 1

[0023] Prepare a PBS buffer solution with a pH of 7.4 and a concentration of 10 mM, and prepare a cresyl violet (CVA) ethanol solution with a concentration of 1 mM with ethanol. The effect of the probe cresyl violet (CVA) on hypochlorite (ClO - ) selectivity. Such as figure 1 As shown, under the excitation condition at 580 nm, the single probe cresyl violet (CVA) (10 µM) has a strong fluorescence emission intensity at 627 nm in PBS buffer solution, and when hypochlorite (ClO - ) (10 eq.), the fluorescence emission intensity at 627 nm decreased significantly, but when other substances (100 µM) were added, the fluorescence emission intensity of the solution system did not change significantly compared with that of the individual probe system. At the same time, it was observed that the individual probe cresyl violet (CVA) was purple in PBS buffer solution, and the addition of hypochlorite changed its color to colorless (attached figure 1 Inset), indicating that the selection o...

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Abstract

The invention provides a method for near-infrared detection of hypochlorite, and concretely relates to a method for detecting hypochlorite (OCl<->) based on a commercial near-infrared dye Cresyl Violet acetate (CVA). The method is characterized in that the hypochlorite is efficiently and selectively detected in a PBS buffer solution with the pH value being 7.4 with the CVA as a fluorescent reagent, and the probe is used for qualitative and semi-quantitative visual detection of trace hypochlorite. The detection method has a high sensitivity to the hypochlorite, the detection limit is as low as5.6 * 10<-8> mol / L, and the detection method has the advantages of simple detection process, strong anti-interference ability, rapidness, sensitiveness, accurate detection result, and broad application prospect in the biomolecular detection field.

Description

technical field [0001] The present invention relates to hypochlorite (ClO - ) detection field, in particular to a reagent and method for near-infrared detection of hypochlorite. Background technique [0002] Hypochlorite and its protonated form of hypochlorous acid are the most important reactive oxygen species in organisms, usually produced by the reaction of hydrogen peroxide and chloride ions, and the whole process requires myeloperoxidase to react Catalyze. Hypochlorite is closely related to our daily life. For example, the main component of common bleach and disinfectant in daily life is sodium hypochlorite. Hypochlorous acid (usually its sodium salt) is widely used in daily life as a bleaching agent, deodorant and disinfectant. As an important reactive oxygen species, hypochlorous acid plays a very important role in maintaining the redox balance in cells, but once the concentration of hypochlorous acid in cells is abnormal, it will cause diseases including rheumato...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6417
Inventor 高光芹谢普会黄家荣王志敏李明旻金显春孟彦羽苏惠
Owner HENAN AGRICULTURAL UNIVERSITY
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