Small intestine liquid used for in-vitro simulation of carbohydrate digestion in small intestine and preparation method thereof

A technique of carbohydrate and in vitro simulation, applied in the direction of measuring devices, instruments, scientific instruments, etc., which can solve the problems of large differences in small intestinal fluid

Inactive Publication Date: 2018-06-15
COFCO NUTRITION & HEALTH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] For this reason, the technical problem to be solved by the present invention is to provide a small intestinal fluid for simulating the digestion of carbohydrates in the small intestine in vit

Method used

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  • Small intestine liquid used for in-vitro simulation of carbohydrate digestion in small intestine and preparation method thereof
  • Small intestine liquid used for in-vitro simulation of carbohydrate digestion in small intestine and preparation method thereof
  • Small intestine liquid used for in-vitro simulation of carbohydrate digestion in small intestine and preparation method thereof

Examples

Experimental program
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Embodiment 1

[0030] The concentration of each component in the small intestinal fluid described in this embodiment is: K + 9.5mmol / L, Na + 154.3mmol / L, Cl - 56.5mmol / L,H 2 PO 4 - 1mmol / L, HCO 3 - 106.3mmol / L, Mg 2+ 0.42mmol / L, Ca 2+ 0.6mmol / L, trypsin 200U / mL, bile salt 10mM, amyloglucosidase 10U / mL.

[0031] The preparation of the small intestine juice described in this embodiment comprises the following steps:

[0032] (1) Weigh 37.3g KCl and dissolve in 1000ml deionized water, 68gKH 2 PO 4 Dissolve in 1000ml deionized water, 84gNaHCO 3 Dissolve in 1000ml deionized water, 117gNaCl Dissolve in 1000ml deionized water, 30.5gMgCl 2 (H 2 O) 6 Dissolve in 1000ml deionized water, 44.1gCaCl 2 (H 2 O) 2 Dissolve in 1000ml deionized water, and about 100ml each of 2M NaOH and 6M HCL, until the above components are fully dissolved;

[0033] (2) Take 17ml KCl solution, 2ml KH solution respectively 2 PO 4 solution, 106.25ml NaHCO 3 solution, 24mlNaCl solution, 2.75mlMgCl 2 (H 2 ...

Embodiment 2

[0036] The concentration of each component in the small intestinal fluid described in this embodiment is: K + 9.5mmol / L, Na + 154.3mmol / L, Cl - 56.5mmol / L,H 2 PO 4 - 1mmol / L, HCO 3 - 106.3mmol / L, Mg 2+ 0.42mmol / L, Ca 2+ 0.6mmol / L, trypsin 200U / mL, bile salt 10mM, amyloglucosidase 10U / mL and invertase 3U / mL.

[0037] The preparation of the small intestine juice described in this embodiment comprises the following steps:

[0038] (1) Weigh 37.3g KCl and dissolve in 1000ml deionized water, 68gKH 2 PO 4 Dissolve in 1000ml deionized water, 84gNaHCO 3 Dissolve in 1000ml deionized water, 117gNaCl Dissolve in 1000ml deionized water, 30.5gMgCl 2 (H 2 O) 6 Dissolve in 1000ml deionized water, 44.1gCaCl 2 (H 2 O) 2 Dissolve in 1000ml deionized water, and about 100ml each of 2M NaOH and 6M HCL, until the above components are fully dissolved;

[0039] (2) Take 17ml KCl solution, 2ml KH solution respectively 2 PO 4 solution, 106.25ml NaHCO 3 solution, 24mlNaCl solution, ...

Embodiment 3

[0042] The concentration of each component in the small intestinal fluid described in this embodiment is: K + 9mmol / L, Na + 160mmol / L, Cl - 50mmol / L, H 2 PO 4 - 1.5mmol / L, HCO 3 - 100mmol / L, Mg 2+ 0.5mmol / L, Ca 2+ 0.5mmol / L, trypsin 250U / mL, bile salt 8mM, amyloglucosidase 15U / mL.

[0043] The preparation method of the small intestinal fluid described in this example is the same as that in Example 1, the only difference being that the small intestinal fluid with the desired ion concentration is obtained by properly adjusting the addition ratio of each raw material.

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Abstract

The invention belongs to the technical field of in-vitro biomimetic simulated digestion and particularly relates to a small intestine liquid used for in-vitro simulation of carbohydrate digestion in the small intestine and further discloses a preparation method thereof. The small intestine liquid used for in-vitro simulation of carbohydrate digestion in the small intestine contains desired concentrations of K<+>, Na<+>, Cl<->, H2PO4<->, HCO3<->, Mg<2+> and Ca<2+> ions, pancreatin, cholate, amyloglucosidase and invertase. The small intestine liquid has the concentrations of inorganic salt ions,digestive enzymes and cholate similar to those of the human small intestine liquid and significantly increases the proportion of carbohydrates digested into glucose compared with the conventional digestive liquid.

Description

technical field [0001] The invention belongs to the technical field of in vitro bionic simulated digestion, in particular relates to a small intestinal juice for in vitro simulated carbohydrate digestion in the small intestine, and further discloses a preparation method thereof. Background technique [0002] Digestion refers to the process in which food is processed into small molecular substances in the digestive tract and then enters the body. There are two main forms of digestion: one is to change food from large pieces to small pieces through mechanical action, which is called physical or mechanical digestion; the other is to change large molecules into small molecules under the action of digestive enzymes , called chemical digestion. Among them, physical digestion mainly occurs in the mouth and stomach, while chemical digestion occurs mostly in the small intestine. At present, the research on the digestion process mainly includes two technical methods: in vivo experim...

Claims

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Application Information

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IPC IPC(8): G01N33/00
CPCG01N33/00
Inventor 孟金凤陈晓东董志忠王勇陈然应剑
Owner COFCO NUTRITION & HEALTH RES INST
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