Liquid chromatographic analysis method for detecting plasma concentrations of oxcarbazepine and oxcarbazepine metabolite in blood

Abstract

The invention discloses a liquid chromatographic analysis method for detecting the plasma concentrations of oxcarbazepine and an oxcarbazepine metabolite in blood. The method comprises the steps thata liquid chromatographic analysis instrument and an ultraviolet detector are used for calibrating a standard solution, and fitting is conducted to obtain a standard curve equation representing the concentration of oxcarbazepine and a standard curve equation representing the concentration of the oxcarbazepine metabolite separately, wherein the standard curve equation representing the concentrationof oxcarbazepine is that y1 is equal to the sum of b and the product of a and x1, and the standard curve equation representing the concentration of the oxcarbazepine metabolite is that y2 is equal tothe sum of d and the product of c and x2; a blood sample to be tested is taken, pretreated and detected by using the same liquid chromatographic analysis instrument and ultraviolet detector to obtainy1 and y2 values of the blood to be tested, the y1 and y2 values of the blood sample to be tested are substituted into the two standard curve equations respectively, the relative plasma concentrationsx1 and x2 of oxcarbazepine and the oxcarbazepine metabolite in the blood sample to be tested are obtained through calculation separately, the concentration of an internal standard substance working solution is known, and the plasma concentrations of oxcarbazepine and the oxcarbazepine metabolite in the blood sample to be tested can be obtained through calculation.

Description

technical field [0001] The invention relates to the technical field of clinical blood drug concentration monitoring of psychotropic drugs, in particular to a method for detecting the content of antiepileptic drug oxcarbazepine and its metabolites in blood. Background technique [0002] Oxcarbazepine (OXC) is a new type of antiepileptic drug, which is a 10-keto derivative of carbamazepine with the molecular formula C 15 h 12 N 2 o 2 , the specific structure is as figure 1 shown. This medicine is indicated for the treatment of partial and generalized tonic-clonic seizures in adults and children over 5 years of age. OXC has the characteristics of good tolerance and less drug-drug interactions, and is gradually replacing traditional antiepileptic drugs alone or in combination. OXC is rapidly absorbed orally, and is almost rapidly degraded into the active metabolite (hereinafter referred to as the metabolite) 10,11-dihydro-10-hydroxycarbazepine (Monohydroxycarbazepine, MHD)...

AI Technical Summary

Problems solved by technology

Among them, high-performance liquid chromatography-mass spectrometry has high analysis costs, high requirements on the site, and requires professionals to operate, so it is not suitable for widespread use in small and medium-sized hospitals.

High-performance liquid chromatography has the advantages of accurate quantification, good reproducibility, and low cost. However, most of the reported methods in the literature have complicated pretreatment operations, and the external standard method may easily lead to inaccurate results and long analysis time. problem, it is not suitable for high-throughput sample detection

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