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A preparation method of fermented seabuckthorn composition capable of improving intestinal immunity

A composition and technology of sea buckthorn are applied in the field of preparation of fermented sea buckthorn compositions, and can solve problems such as lack of a good repair method and the like

Active Publication Date: 2021-05-04
SHANXI JINKEHAI BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above existing technologies and related research results all use probiotics to protect or repair general gastrointestinal damage, and for intestinal damage caused by oxidative stress, especially for the generation of a large number of free radicals Intestinal mucosal injury, currently there is no good repair method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] a) Mix seabuckthorn leaves and seabuckthorn fruits with a weight ratio of 1:1, then grind, add 0.5 times the weight of water and stir well, then add a mixture of cellulase and dextranase with 1% of the total weight of seabuckthorn leaves and seabuckthorn fruits Enzyme, after enzymolysis at 35°C for 4 hours, the enzymolysis solution was obtained by filtration.

[0027] b) Mix seabuckthorn leaves and seabuckthorn fruits with a weight ratio of 1:1, then grind, add 0.5 times the weight of water and stir well, then add Acetobacter pasteurii (CICC20056) and rice with 0.1% of the total weight of seabuckthorn leaves and seabuckthorn fruits The mixed bacteria of Rhizopus (CICC3010) was aerobically fermented at 25°C for 12 hours, and the fermentation broth was obtained by filtration after fermentation.

[0028] c) leaching with 75% ethanol at 40°C for 12 hours to obtain seabuckthorn leaf extract.

[0029] d) Concentrating the enzymolysis solution, fermentation solution and seabu...

Embodiment 2

[0033] a) Mix fresh seabuckthorn leaves and seabuckthorn fruits with a weight ratio of 1:3, then grind, add 2 times the weight of water and stir well, then add 3% of the total weight of seabuckthorn leaves and seabuckthorn fruits. Cellulase and mannanase Mix enzymes, digest at 65°C for 12 hours, and filter to obtain the hydrolyzate.

[0034] b) Mix fresh seabuckthorn leaves and seabuckthorn fruits with a weight ratio of 2:1, then grind, add 1 times the weight of water and stir well, add Acetobacter pasteurii (CICC20056) and 0.5% of the total weight of seabuckthorn leaves and seabuckthorn fruits The mixed bacteria of Saccharomyces cerevisiae (CICC1001) was aerobically fermented at 35°C for 48 hours, and the fermentation broth was obtained by filtration after fermentation.

[0035] c) leaching with 70% ethanol at 40°C for 10 hours to obtain seabuckthorn leaf extract.

[0036] d) Concentrating the enzymolysis solution, fermentation solution and seabuckthorn leaf extract to a wat...

Embodiment 3

[0040] a) Mix fresh seabuckthorn leaves and seabuckthorn fruits with a weight ratio of 1:2, then grind, add 1.5 times the weight of water and stir well, then add cellulase, mannanase and 2% of the total weight of seabuckthorn leaves and seabuckthorn fruits Mixed enzymes of dextranase, after enzymolysis at 50°C for 8 hours, the enzymolysis solution was obtained by filtration.

[0041] b) Mix the fresh seabuckthorn leaves and seabuckthorn fruits with a weight ratio of 1.5:1, then grind, add 1 times the weight of water and stir well, add Acetobacter pasteurii (CICC20056) with 0.3% of the total weight of seabuckthorn leaves and seabuckthorn fruits, The mixed bacteria of Saccharomyces cerevisiae (CICC1001) and Rhizopus oryzae (CICC3010) were aerobically fermented at 30°C for 30 hours, and the fermentation broth was filtered after fermentation.

[0042] c) leaching with 70% ethanol at 45°C for 5 hours to obtain seabuckthorn leaf extract.

[0043] d) Concentrating the enzymolysis solu...

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Abstract

The invention discloses a preparation method of a fermented seabuckthorn composition capable of improving intestinal immunity, and relates to the technical field of biological fermentation compositions. Seabuckthorn leaves and seabuckthorn fruits are subjected to biological bacteria fermentation, enzymatic hydrolysis, and organic matter extraction, respectively, to obtain The fermented liquid, enzymatic solution and seabuckthorn leaf extract of seabuckthorn leaves and seabuckthorn fruits are mixed with probiotics to make finished products through freeze-spray drying; Microbial metabolism provides nutrition and material basis. At the same time, combined with the rich antioxidant active substances in seabuckthorn leaves, the beneficial bacteria are firmly wrapped in the drying technology, which can effectively improve the activity of beneficial bacteria reaching the intestinal tract and produce effective antioxidant properties. properties; the present invention can be applied to products that improve intestinal autoimmunity.

Description

technical field [0001] The invention relates to the technical field of biological fermentation compositions, in particular to a preparation method of a fermented seabuckthorn composition capable of improving intestinal immunity. Background technique [0002] The intestinal tract is not only the main place for digestion and absorption of nutrients in the human body, but also the most vulnerable target organ in multiple organ dysfunction syndrome and systemic inflammatory response syndrome. The intestinal mucosal barrier of normal human body is composed of mechanical barrier, biofilm barrier and immune barrier. A complete intestinal mucosal defense system can effectively prevent harmful substances in the intestinal tract from entering the systemic circulation. At present, when the human body is under stress, its intestines will produce a large number of free radicals, which will cause damage to the intestinal mucosa. Furthermore, the clinical manifestations of intestinal muc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L33/00A23L33/10A23L33/105A23L19/00
CPCA23L19/07A23L33/00A23L33/10A23L33/105A23L33/135A23V2002/00A23V2400/125A23V2400/515A23V2200/3204A23V2200/32A23V2200/324A23V2250/21
Inventor 郭海利
Owner SHANXI JINKEHAI BIOLOGICAL PROD
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