Real-time controllable micro-droplet reciprocated conveying device and method based on lithium niobate sandwich structure chip

Abstract

The invention discloses a real-time controllable micro-droplet reciprocated conveying device and method based on a lithium niobate sandwich structure chip. The device comprises a laser 1, an electronic shutter 2, a diaphragm 3, an optical reshaper 4, a thermosensitive probe 5, an intelligent digital display temperature controller 6, a background light source 7, a transparent fine-adjustment three-dimensional translation platform 8, a semiconductor refrigerating plate 9, the lithium niobate sandwich structure chip 10, an objective lens 11, a laser reflector 12, an optical filter 13, and a CCD camera 14. The device is combined by three parts of a micro-droplet reciprocated conveying optical path, a real-time observation optical path and a temperature control device. Through the combined action of a heat effect and a photovoltaic effect, the micro-droplet reciprocated conveying is realized. The method has the characteristics of controllable micro-droplet conveying direction and distance,no direct contact between the laser and the micro-droplets and the like, and the whole process can be observed in real time. The technology can be applied to the collection mixing and conveying of a microscale reagent, and has important significance on the development of the fields, such as biological medical treatment, pharmacodiagnosis, environment monitoring and molecular biology.

Description

technical field [0001] The invention relates to a micro-droplet control technology, specifically a real-time controllable micro-droplet reciprocating transport device and method based on a lithium niobate sandwich structure chip Background technique [0002] With the rapid development of microfluidic chips, micro-droplet manipulation has become a research hotspot in this field. Micro-droplet manipulation technology is mainly used in the analysis and detection of micro-sample in biological, chemical, pharmaceutical and other processes. It mainly involves the collection, mixing and transportation of micro-quantity reagents. It is of great significance to the development of fields such as biomedicine, drug diagnosis, food hygiene, environmental monitoring and molecular biology. [0003] 2003 document (Monolithic membrane valves and diaphragm pumps for practical large-scale integration into glass microfluidic devices, Sens.ActuatorsB, 89, 315-323 (2003)) reported a method of ut...

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Problems solved by technology

[0007] There are many shortcomings in the micro-droplet transport methods reported so far, such as: the need to prepare micro-channels, micro-chambers and other devices, high cost and complicated processing technology; the direction and distance of micro-droplet transport are uncontrollable; The long-term irradiation of the droplet will cause the internal environment of the droplet to be disordered; the environment of the micro-droplet is limited; the reciprocating transport of the micro-droplet cannot be realized; the transport process cannot be controlled in real time, etc.

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