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Toxoplasma Colloidal gold immunochromatographic test strip and preparation method thereof

An immunochromatographic test paper and colloidal gold technology, applied in the biological field, can solve the problems of low detection rate, complicated operation, and low sensitivity

Inactive Publication Date: 2018-07-10
SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the common diagnostic methods for toxoplasmosis include etiological methods, molecular biological methods, immunological methods, etc., but there are many shortcomings such as low detection rate, complicated operation, and low sensitivity. In order to meet the needs of large-scale general surveys at the grassroots level, It is necessary to develop a rapid detection kit for a variety of animals in order to detect Toxoplasma gondii simply and conveniently

Method used

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  • Toxoplasma Colloidal gold immunochromatographic test strip and preparation method thereof
  • Toxoplasma Colloidal gold immunochromatographic test strip and preparation method thereof
  • Toxoplasma Colloidal gold immunochromatographic test strip and preparation method thereof

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Embodiment 1

[0043] 1 Materials and methods

[0044] 1.1 Materials

[0045] 1.1.1 Plasmids and strains

[0046] E.coli BL21(DE3) was purchased from Beijing Quanshijin Biotechnology Co., Ltd.; plasmid PET-28a(+) was preserved in the laboratory.

[0047] 1.1.2 Related reagents

[0048] Restriction enzymes BamHI, HindIII, T4 DNA ligase, and 2×PCR mix were purchased from TaKaRa Bioengineering (Dalian) Co., Ltd.; ProteinRuler II (12-120 kDa) was purchased from Beijing Quanshijin Biotechnology Co., Ltd.; Toxoplasma gondii Rat positive serum was isolated and preserved in the laboratory; goat anti-mouse IgG-HRP was purchased from Beijing Kangwei Century Biotechnology Co., Ltd.; recombinant Protein G was purified and preserved in the laboratory. Nitrocellulose membrane (Sartorius CN140), glass cellulose membrane (GL0194), and PVC rubber sheet (DB-6) were purchased from Shanghai Jieyi Biotechnology Co., Ltd.; chloroauric acid was purchased from Sigma; other chemical reagents were of analytical gr...

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Abstract

The invention provides a Toxoplasma colloidal gold immunochromatographic test strip, comprising a sample pad, a colloidal gold binding pad, a detection line, a quality control line and a water-absorbing material which are arranged sequentially along a sample flowing direction, wherein the detection line includes a toxoplasma specific antigen. The Toxoplasma colloidal gold immunochromatographic test strip allows Toxoplasma in human and various mammals to be quickly detected in simple and easy way.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular, the invention relates to a toxoplasma gondii colloidal gold immunochromatographic test strip and a preparation method thereof. Background technique [0002] Toxoplasma gondii is a widespread, obligate intracellular parasite, which can be transmitted vertically through the placenta after infecting humans or animals, causing fetal malformation, premature birth, and developmental disorders of the nervous system; it can also be transmitted orally, such as drinking untreated Sterilized dairy products, or the use of undercooked meat; the host selection is not strong, it can parasitize in the nucleated cells of a variety of animals, and invade a variety of tissues and organs. Among them, sheep are more susceptible animals. After infection, they show abortion, stillbirth and weak animals, which pose a serious threat to humans and the breeding industry. [0003] In order to find a sensitive, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543G01N33/531
CPCG01N33/531G01N33/54306G01N33/558G01N2333/45
Inventor 朱传刚王钊哲许瑞林矫矫陈兆国洪炀陆珂李浩吴思敏李嘉静江嘉欣
Owner SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI
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