Constructing method of fetal-original bipolar disorder rat model and application thereof

A construction method and rat model technology, applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, organic active ingredients, etc., can solve the problems of mouse sleep loss, increased irritability, and increased aggressiveness of mice, etc. Achieve the effect of strong repeatability, simple modeling method and good stability

Active Publication Date: 2018-07-13
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the existing BD animal models are treated with different intervention factors in adulthood, such as the administration of psychoactive drugs opioid peptides and withdrawal of drugs after treatment, resulting in mice being sensitive to small stimuli, that is, increased irritability; Treatment with amphetamine, a stimulating drug, caused sleep loss in mice; keeping mice in isolation increased their aggressiveness...

Method used

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  • Constructing method of fetal-original bipolar disorder rat model and application thereof
  • Constructing method of fetal-original bipolar disorder rat model and application thereof
  • Constructing method of fetal-original bipolar disorder rat model and application thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0036] [Example 1] Construction of animal model of fetal bipolar disorder of the present invention

[0037] 1. Experimental animals:

[0038] SPF grade healthy Wistar rats were purchased from Hubei Provincial Center for Disease Control and Prevention, animal license number: SCXK (E) 2008-2010. This study was approved by the Ethics Committee of Wuhan University Medical Center, and was carried out in strict accordance with the relevant treatment guidelines of international laboratory animal protection certification and evaluation institutions.

[0039] The experimental animals were kept in a barrier environment with a temperature of 22-25° C. and a humidity of 50%, with 12 hours of day and night alternation.

[0040] 2. Experimental method:

[0041] 10 male Wistar rats (body weight 260-300g), and 20 female Wistar rats (body weight 200-240g). Free to drink and eat, after 7 days of adaptive feeding, according to male: female = 1:2 cage, vaginal smear in the next morning, to det...

Embodiment 2

[0086] [Example 2] Using the model of the present invention to screen environmental interferents or drugs for fetal metabolic syndrome

[0087] 1. The fetal bipolar disorder model established according to the method of embodiment 1;

[0088] 2. Apply environmental disruptors or drugs to animal models;

[0089] 3. Observe the influence of candidate environmental disruptors or drugs on various indicators of fetal-derived bipolar disorder, and evaluate potential environmental disruptors or drugs for fetal-derived bipolar disorder.

[0090] In summary, the modeling method of the present invention is treated with dexamethasone during pregnancy, and the offspring are routinely raised up to 10 weeks after birth, and given chronic stimulation by swimming in ice water at 10-12 weeks, the experimental group has abnormal activation of the HPA axis, anxiety and depression It is an effective method to establish the diagnosis of fetal-derived bipolar disorder, and it can be used to guide c...

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Abstract

The invention relates to a constructing method of a fetal-original bipolar disorder animal model and application thereof. The fetal-original bipolar disorder animal model is characterized in that a rodent animal is injected with 0.1 to 2.0mg/kg of dexamethasone in a subcutaneous way 9 to 20 days after getting pregnant; the rodent animal is conventionally and normally fed to 10 weeks after birth, the chronic stimulation by ice water swimming is performed for two weeks, and then the typical feature similar to human bipolar disorder appears. The fetal-original bipolar disorder animal model is novel, reliable, simple and convenient, and has important meaning on the instruction of clinical reasonable drug utilization in pregnant period, study of fetal-original bipolar disorder action mechanismand molecular targets, screening of intrauterine environment interference matters and medicines, and the like.

Description

technical field [0001] The invention relates to the technical field of animal model construction, in particular to a method for constructing an animal model of fetal bipolar disorder. Background technique [0002] Bipolar disorder, also known as bipolar disorder (BD), is a mental disorder characterized by abnormal mania or depression. During a manic episode, it manifests as elevated mood, lack of sleep, increased sexual activity, and increased activity; while during a depressive episode, it manifests as depressed mood, slowed thinking, and decreased activity. In severe cases, psychotic symptoms such as hallucinations, delusions, or catatonic symptoms may appear during the acute phase of the attack. Clinically, it can be distinguished by these characteristic manifestations of the disease [1,2] . According to the classification criteria in Chapter 5 of the Diagnostic and Statistical Manual of Diseases (DSM-5), BD is divided into three types: 1) Bipolar disorder type I, with...

Claims

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Application Information

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IPC IPC(8): A61K31/573A61K49/00
CPCA61K31/573A61K49/0008
Inventor 徐丹汪晖余樱董婉婷胡泽文
Owner WUHAN UNIV
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