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A kind of rapid propagation method of kansui

A fast and kansui technology, applied in the biological field, can solve the problems of low reproduction rate, low germination rate, and slow reproduction speed, and achieve the effects of high reproduction coefficient, high germination efficiency, and fast seedling raising speed

Active Publication Date: 2021-06-01
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] At present, there are a lot of researches on the organ structure and development, chemical composition, pharmacological effects and clinical application of Kansui. Although, in traditional cultivation, Kansui seeds are difficult to germinate and have a low germination rate, so , the reproduction rate is low, the reproduction speed is slow, and the seed consumption is large, but there is no report and application of any effective cultivation method to improve its yield

Method used

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  • A kind of rapid propagation method of kansui
  • A kind of rapid propagation method of kansui
  • A kind of rapid propagation method of kansui

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Embodiment Construction

[0030] 1 Screening of disinfection conditions and treatment of sterile materials contaminated with bacteria

[0031] 1.1 Selection of explants and screening of disinfection conditions

[0032] The explant material is seriously infected with bacteria in autumn and winter every year, which makes disinfection difficult and the survival rate is extremely low. Therefore, stem segments or leaves from March to April are selected as explants.

[0033] After exploring the disinfection conditions, it is found that when young leaves and stems are used as explants, the effect of soaking in 70% ethanol for 10s is the best, and the time must be strictly controlled at 10s, because as time goes on, the explants will be polluted. Although the dehydration rate decreased, the dehydration gradually increased, the browning rate increased, and the final material died (Table 1).

[0034] Table 1 Effects of 70% ethanol at different times on Kansui explants

[0035]

[0036] After the initial dis...

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Abstract

The invention discloses a rapid propagation method of Kansui, which uses the stem section with the axillary bud primordium removed as the explant, and firstly in the culture medium (MS+6-BA 1.0mg / L+IAA 3.0mg / L ) to induce clustered buds; when the clustered buds were 5cm in height, they were cut into 1cm-long single buds, placed in medium (MS+6‑BA 1.0mg / L+NAA 0.2mg / L) for strong seedlings Cultivate; when the single bud grows to 5-7 pieces of leaves, cut off the leafy segment with a length of 2cm at the top and place it in (1 / 2MS+IBA 0.5mg / L) or (1 / 2MS+IBA 1.0mg / L ) for rooting culture. The method has high germination efficiency, high propagation coefficient and fast seedling raising speed, and can provide a large amount of seedlings for artificial cultivation of Gansui and reduce damage to wild resources thereof.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a method for rapid propagation of kansui. Background technique [0002] Gansui (Euphorbia kansui Liou) is a perennial herb of the genus Euphorbia (Euphorbiaceae) in the family Euphorbiaceae. 1974]. Its medicinal value was first recorded in "Shen Nong's Materia Medica" [Shen Nong's Materia Medica, 1995]. The part used as medicine is the dry root tuber, which is bitter and cold and poisonous. Medicinal effects such as laxative. Today, Gansui is concentrated in Shanxi, Shaanxi, Henan, Ningxia, and Gansu. Among them, Shaanxi is the main producing area of ​​excellent Gansui [Quan Yishu, 1994], and it mostly grows on field ridges, low hillsides, sandy land, and roadsides. In recent years, studies have found that Gansui milk contains a variety of effective chemical components, such as diterpene lipids, which can significantly resist cancer and viruses [Dickenson and Fairbaim, 1975; Pan Qin ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 蔡霞王萌王豆张悦方晓艾李鹏张清
Owner NORTHWEST UNIV