Tissue culture and rooting method of saline-alkaline tolerant fast-growing ulmus pumila clonal line adventitious buds
A saline-alkali-resistant and clone technology, which is applied in the field of saline-alkali-resistant fast-growing Ulmus asexual clone adventitious bud tissue culture and rooting, can solve the problems of troublesome rooting medium preparation, waste, easy precipitation, etc., to reduce production costs and reduce dosage , the effect of vigorous growth
Active Publication Date: 2018-07-24
SHANDONG JIANZHU UNIV +1
View PDF9 Cites 1 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
But, because the adventitious buds of Ulmus clonale that are used for rooting in the bottle have strict requirements (the height of the seedling must be 2-3.5cm, robust, individuals with stretched leaves), it causes great waste in actual production
Activated carbon is very easy to precipitate when the medium solidifies, causing unnecessary troubles to the preparation of rooting medium
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0031] (1) Selection of adventitious buds for rooting: select the adventitious buds of the saline-alkali-tolerant fast-growing Ulmus ultifolia clone (the growth period is less than one week) through normal growth, more than 1.5 cm in plant height, and through multiple successive cultures (or callus differentiation formation). except for tender tissues);
[0032] (2) Adventitious bud inoculation: Inoculate the adventitious buds of the saline-alkali-tolerant Ulmus clonal clone in EM rooting medium for rooting culture.
[0033] The growth environment is: 26°C (±2°C) during the day / 18°C (±2°C) at night, 12 hours of light per day, light intensity of 2000-3500 lux, and relative air humidity of 90%.
[0034] EM rooting medium includes the following components and their contents: CaCl 2 2H 2 O 220.5mg / L, NH 4 NO 3 800mg / L, KNO 3 1011mg / L, MgSO 4 1680.5mg / L, NaH 2 PO 4 2H 2 O 156mg / L, K 2 SO 4 175mg / L, Na 2 SO 4 150mg / L, CaCl 2 115mg / L, CoCl 2 ·6H 2 O 0.24mg / L, Cu...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention belongs to the technical field of plant culture and particularly relates to a tissue culture and rooting method of saline-alkaline tolerant fast-growing ulmus pumila clonal line adventitious buds. The saline-alkaline tolerant fast-growing ulmus pumila clonal line adventitious bud tissue culture and rooting method comprises selection and inoculation of saline-alkaline tolerant fast-growing ulmus pumila clonal line adventitious buds, wherein the rooting conditions of the adventitious buds are as follows: rooting is conducted in a formula (EM rooting+IBA1mg / L+2 percent S) researchedand developed by the invention, the culture temperature is 26 DEG C (+ / -2) DEG C in daytime and 18 DEG C (+ / -2) DEG C at night, cultivation is conducted in the environment with the relative air humidity of 90 percent, illumination is conducted for 12 hours per day, and the illumination intensity is 2000 to 3500 lux. By adoption of the formula and the method, the rooting rate of the ulmus pumila adventitious buds reaches to 96 percent; furthermore, the utilization rate of the adventitious bud materials for rooting is increased by more than 20 to 35 percent compared with that in earlier stage.
Description
technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for tissue culture and rooting of adventitious buds of saline-alkali-tolerant fast-growing Ulmus ulmus clones. technical background [0002] White elm (Ulmus pumila L.) is a plant of the genus Ulmus in the family Ulmaceae, with many varieties. [0003] Saline-alkali land is a type of land where salt accumulates in the soil and the salt contained in the soil has affected the normal growth of plants. The area of saline-alkali land in my country is 3.5×10 7 hm 2 , equivalent to 33.3% of the arable land area. How to effectively use the ecological environment in saline-alkali areas and increase the coverage of woody plants in saline-alkali areas is one of the important problems faced by forestry workers. As one of the main ornamental tree species in gardens in my country, white elm is highly resistant to toxic gases such as smoke, sulfur dioxide an...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 慕德宇梁玉王强范小莉房用慕宗昭林大为冒文娟
Owner SHANDONG JIANZHU UNIV