Double-doped fluorescent carbon quantum dots, synthetic method and serum iron content detection method
A technology of carbon quantum dots and a synthesis method, applied in the field of analysis and detection, can solve the problems of ineffective application of iron ion detection, cumbersome detection process operation, weak anti-interference ability, etc., so as to overcome poor light stability, improve detection efficiency, and reliable results Effect
Active Publication Date: 2018-07-27
SHENZHEN UNIV
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The first three methods require sophisticated instruments and professional and skilled operations and are not suitable for clinical serum iron detection; colorimetry is a common method for clinical iron content detection, which has the advantages of high sensitivity and good selectivity, but at the same time The detection process is complicated to operate, the anti-interference ability is weak, and some reagents are expensive.
Although the fluorescence detection method has excellent quantitative detection ability, the existing fluorescent probes for iron ions are mainly organic fluorescent substances, which have poor photostability and weak anti-interference ability and cannot be effectively applied to the detection of iron ions in complex biological samples.
Although there are also reports on carbon quantum dot fluorescent probes that respond to iron ions, biomolecules such as proteins in biological samples have a greater impact on the fluorescence of quantum dots, so most quantum dots that respond to iron ions can only be applied to simple samples. Iron ion detection
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[0028] A double-doped fluorescent carbon quantum dot can be synthesized as follows:
[0029] Step 1 Mix 100 μL of 3-aminopropyltriethoxysilane and 150 μL of ethylenediamine in 20 mL of deionized water, then place the uniformly mixed solution in a 50 mL hydrothermal reactor, and react at a temperature of 200°C for 5 hours to obtain Quantum dot crude product.
[0030] In step 2, the crude quantum dot product obtained in step 1 is subjected to freeze-drying treatment to obtain a powder solid product, and a double-doped fluorescent carbon quantum dot containing water-insoluble impurities is obtained. In the embodiment, the freeze dryer is Boyikang Lab-1A-80E, and the crude quantum dot product obtained in step 1 is pre-frozen at -80°C for 24 hours and then placed in the freeze dryer for 12 hours.
[0031] Step 3 Add the solid powder product obtained in Step 2 into 20 mL of deionized water and stir to dissolve for 30 min, then centrifuge the dissolved solution at a speed of 15,000 ...
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The invention discloses double-doped fluorescent carbon quantum dots. The optimal excitation and emission wavelengths of the double-doped fluorescent carbon quantum dots are 325 nm and 390 nm respectively, atomic groups of the double-doped fluorescent carbon quantum dots are 3-6 nm; the double-doped fluorescent carbon quantum dots are prepared from 58.74% by weight of C, 22.53% by weight of O, 10.19% by weight of N and 8.54% by weight of Si. Meanwhile, the invention discloses a simple and convenient synthetic method of the double-doped fluorescent carbon quantum dots and a method for detectingiron content of serum with a standard curve method on the basis of the double-doped fluorescent carbon quantum dots. Detection process is simplified, detection efficiency is increased, and the problem that an iron ion fluorescent probe in a fluorescence detection method is poor in light stability and low in anti-interference capability in a complex biological sample is solved.
Description
technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a double-doped fluorescent carbon quantum dot and a synthesis method thereof, and a method for detecting iron content in serum by using the double-doped fluorescent carbon quantum dot. Background technique [0002] In the prior art, methods for detecting iron content in samples include inductively coupled plasma mass spectrometry (ICPMS), atomic emission spectroscopy (AES), atomic absorption spectroscopy (AAS) and colorimetry. Inductively coupled plasma mass spectrometry (ICPMS) and atomic emission spectrometry (AES) are used to quantitatively analyze the iron content in the sample by measuring the characteristic radiation emitted by the excited iron atoms when they return to the ground state after the sample is atomized; atomic absorption Spectroscopy (AAS) is a method of quantitative analysis by measuring the absorption degree of the characteristic radiation line...
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IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 陈雯雯何松良
Owner SHENZHEN UNIV