A method for establishing a new soybean germplasm with double deletion of lipoxygenase and 7s globulin

Abstract

The invention discloses a method for constructing lipoxygenase and 7S globulin double-deletion soybean germplasm. According to the method, soybean cultivars with three subunits including alpha, alpha'and beta of 7S globulin deleted simultaneously are acquired through screening from soybean germplasm with an improved polyacrylamide gel electrophoresis method, Dongfu soybeans 3 with three isozymesof lipoxygenase deleted simultaneously are taken as a female parent, soybean cultivars with three subunits of 7S globulin deleted simultaneously are taken as a male parent, and the female parent and the male parent are subjected to hybridized combination. By means of the improved polyacrylamide gel electrophoresis method, deletion detection on 7S globulin and lipoxygenase is performed, and the newsoybean germplasm with lipoxygenase and 7S globulin deleted simultaneously is obtained. By means of the method, the detection cost can be reduced, the detection time is saved by 2 times or longer, discharge of detection wastes can be reduced by 2 times or more, environmental pollution is reduced, more importantly, the problem about low generation analysis detection in the gene pyramid breeding process is solved, and the best detection method is provided for breeding improvement of soybean quality.

Description

technical field [0001] The invention relates to a method for detecting soybean germplasm, in particular to a method for simultaneously detecting double deletion of lipoxygenase and 7S globulin in soybean. The invention belongs to the technical field of agricultural production and processing. Background technique [0002] For a long time, my country's soybean deep-processing enterprises, such as soybean protein isolate enterprises, soybean flour processing enterprises, vegetarian meat processing enterprises, etc., have no special soybean raw materials for processing, and the gelation and stability of the soybean protein isolate produced by the products are poor. The fishy smell is difficult, which leads to the shortening of the shelf life (shelf life) of the meat products produced by the meat product enterprises that use protein isolate as an additive, and it is difficult to remove the fishy smell of the product. In order to prolong the shelf life of the product, the enterpris...

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Problems solved by technology

[0004] The prior art method for analyzing and detecting the absence of 7S globulin subunit and lipoxygenase must be separately sampled and detected separately if it is to be determined simultaneously whether this component is missing or not. For the same seed, after two samples are taken, even if the obtained The analysis results of the test meet the requirements of the breeding goal, and the seeds after sampling need to be propagated to offspring. The seeds will lose their vitality due to excessive damage, resulting in breeding failure.

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