A kind of quality evaluation method of ginkgo biloba and its extract and preparation
A technology for quality evaluation and extraction, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of lack of reference substances, inability to objectively and reasonably control the quality of Chinese medicinal materials and their preparations, etc. Detecting the effect of ingredients
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Embodiment 1
[0042] Combine below figure 2 And examples are described in detail:
[0043] 1) Reference substance
[0044] Quercetin-3-O-(2'',6"-di-O-α-L-rhamnosyl)-β-D-glucoside (1), kaempferol-3-0-(2' ',6"-di-O-α-L-rhamnosyl)-β-D-glucoside (2), quercetin-3-O-rutose-7-O-glucoside (3), Quercetin 3-O-glucoside (4), Isorhamnetin-3-O-β-D-rutinoside (5), Kaempferol 3-(2''-β-D-glucoside)-α -L-rhamnoside (6), quercetin 3-O-α-(6'''-p-coumarin-glucoside-β-1,4-rhamnoside) (7), kaempferol 3-O-α-(6'''-p-coumarolyl-glucoside-β-1,2-rhamnoside) (8).
[0045] 2) Preparation of sample solution
[0046] Accurately weigh 5.0 mg of Ginkgo biloba extract powder in a volumetric flask, add methanol to dissolve it, sonicate for 5 min, dilute to 20 mL, shake well, filter with 0.22μm microporous membrane, and use high performance liquid chromatography Perform analysis and testing.
[0047] 3) Preparation of reference solution
[0048] Accurately weigh each 1.0 mg of the eight ginkgo flavonoids in step 1), add methanol t...
Embodiment 2
[0056] Combine below image 3 And examples are described in detail:
[0057] 1) Reference substance
[0058] Quercetin-3-O-(2'',6"-di-O-α-L-rhamnosyl)-β-D-glucoside (1), kaempferol-3-0-(2' ',6"-di-O-α-L-rhamnosyl)-β-D-glucoside (2), quercetin-3-O-rutose-7-O-glucoside (3), 3'-methyl-myricetin-3-O-rutinoside (4), quercetin 3-O-glucoside (5), kaempferol-3-O-rutinoside (6), isorhamnetin- 3-O-β-D-rutinoside (7), kaempferol 3-(2''-β-D-glucoside)-α-L-rhamnoside (8), quercetin 3-O-α -(6'''-p-coumarin-glucoside-β-1,4-rhamnoside)(9), kaempferol 3-O-α-(6'''-p-coumarin- Glucoside-β-1,2-rhamnoside) (10).
[0059] 2) Preparation of sample solution
[0060] Accurately weigh 5.0 mg of Ginkgo biloba extract powder in a volumetric flask, add methanol to dissolve it, sonicate for 5 min, dilute to 20 mL, shake well, filter with 0.22μm microporous membrane, and use high performance liquid chromatography Perform analysis and testing.
[0061] 3) Preparation of reference solution
[0062] Precisely weigh...
Embodiment 3
[0070] Combine below Figure 4 And examples are described in detail:
[0071] 1) Reference substance
[0072] Quercetin-3-O-(2'',6"-di-O-α-L-rhamnosyl)-β-D-glucoside (1), kaempferol-3-0-(2' ',6"-Di-O-α-L-rhamnosyl)-β-D-glucoside (2), kaempferol-3-O-β sophoroside (3), quercetin-3-O -Rutinose-7-O-glucoside (4), 3'-methyl-myricetin-3-O-rutinoside (5), quercetin 3-O-glucoside (6), quercetin 3 -O-(2-β-D-glucosyl-α-L-rhamnoside) (7), kaempferol-3-O-rutinoside (8), isorhamnetin-3-O-β-D -Rutin (9), Kaempferol 3-(2''-β-D-glucoside)-α-L-rhamnoside (10), Quercetin 3-O-α-(6'''- p-coumarinyl-glucoside-β-1,4-rhamnoside) (11), kaempferol 3-O-α-(6'''-p-coumarin-glucoside-β-1, 2-rhamnoside) (12).
[0073] 2) Preparation of sample solution
[0074] Accurately weigh 5.0 mg of Ginkgo biloba extract powder in a volumetric flask, add methanol to dissolve it, sonicate for 5 min, dilute to 20 mL, shake well, filter with 0.22μm microporous membrane, and use high performance liquid chromatography Perfor...
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