Cardiac troponin kit based on microfluidic chip, preparation method thereof and detection method of cardiac troponin

Abstract

The invention discloses a cardiac troponin kit based on a microfluidic chip, a preparation method thereof and a detection method of cardiac troponin. The preparation method comprises the steps of (1)coating the microfluidic chip; (2) performing fluorescent microsphere labeling; (3) assembling the microfluidic chip; (4) preparing a calibration article; (5) acquiring the cardiac troponin cTn1 kit based on the microfluidic chip. The cardiac troponin cTn1 kit performs measuring by means of a double-antibody sandwich method; high-sensitivity time-resolved phosphor is selected as a marker to perform antibody marking on fluorescent microspheres; analytical detection is performed via antibody pair immunoreaction; the performance of a reagent prepared herein may reach the level of same chemical light-emitting reagents. After immunoreaction, a washing liquid is used to fully remove surplus components; detecting and reading are performed on the fluorescent microspheres under immunoreaction bonding; the problem is avoided that the introduction of a developing liquid into the chip causes insufficiency of a chip or failure of timely post-rendering reading.

Description

technical field [0001] The invention belongs to the field of immune detection, and in particular relates to a cardiac troponin detection kit based on a microfluidic chip and a preparation and detection method thereof. Background technique [0002] Microfluidic chip technology is characterized by a micropipeline network, using micromachining technology to etch a micropipeline network and other functional units on a chip of a few square centimeters in size, thereby preparing a microfluidic chip that includes injection, reaction, separation, and detection An integrated fast, efficient, and low-consumption micro-analysis device. The microfluidic chip has the characteristics of less reagent consumption, short reaction time and high degree of automation in the detection platform. In the current research, the miniaturization of the chip has brought many difficulties to the early sample separation and supporting integration, and there are also many problems in sample fixation and e...

AI Technical Summary

Problems solved by technology

[0011] Using other existing technologies or (1) the structure of the chip is too complicated to precisely regulate the reaction, and there is a large pollution in the cleaning steps and liquid conduction; or (2) multiple liquid chambers and solid phase carriers are used (magnetic beads and fluorescent microspheres are used at the same time), the overall design of the reagent is too complicated; or (3) the immunoreaction adopts a heterogeneous reaction, which means that the solid phase carrier reacts with the surface of the solid phase carrier, the reaction is insufficient, and the sensitivity and Repeatability is limited

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