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Abalone blood cell processing method

A processing method and technology of blood cells, applied in cell dissociation methods, blood/immune system cells, animal cells, etc., can solve problems such as delays in the detection process, increased test costs, and blockage of detectors

Active Publication Date: 2018-08-14
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, untreated cell populations are likely to cause blockage of the detector, and it takes a long time to clean the detector (10-40min) before continuing to test samples
This not only delays the testing process, but also invisibly increases the testing cost (200 yuan / h)

Method used

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  • Abalone blood cell processing method
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  • Abalone blood cell processing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A blood filter such as figure 1 , 2 As shown, it includes a fixed table 1, a vertical rod 2, a filter device 3 and a storage device 4, the storage device 4 is placed under the filter device 3, and the filter device 3 is installed on the vertical rod 2 through a pressure ring rod 5, Holder rod 5 is installed on the vertical rod 2 through the height adjustment button 14, and the vertical rod 2 is installed on the fixed table 1, and the filter device 3 includes an upper hold-down ring 6 and a lower hold-down ring 7, and the upper hold-down ring 6 and the lower hold-down ring The pressure ring 7 is all connected with the vertical rod 2. A silk screen is arranged between the upper pressing ring 6 and the lower pressing ring 7 . The grid mesh 13 filters the blood through the sieve silk.

[0046] Further, in order to facilitate the replacement of the sieve silk arranged between the upper pressing ring 6 and the lower pressing ring 7, the upper pressing ring 6 is provided wi...

Embodiment 2

[0054] A method for processing abalone blood cells, said processing method comprising the steps of:

[0055] (1) First add 1ml of anticoagulant protectant to a 2ml centrifuge tube;

[0056] (2) Then use a scalpel to cut open the blood sinuses of the abalone's gastropod, and place the abalone on ice at an angle of 20-30° so that the blood can flow out more easily;

[0057] (3) After the blood flows out, draw 1ml of blood with a sterile syringe and directly inject it into the centrifuge tube after adding the anticoagulant protective agent;

[0058] (4) adopt the liquid in the hemofilter filtration step (3) in the embodiment 1 to the new 2ml centrifuge tube;

[0059] (5) centrifuge at 18°C ​​and 3000rpm for 10min, and remove the supernatant;

[0060] (6) Finally, resuspend the blood cells treated in step (5) with 1 ml of bacteria-filtered seawater to make abalone hemolymph suspension.

[0061] Wherein, the anticoagulation protective agent is composed of the following component...

Embodiment 3

[0068] A method for processing abalone blood cells, said processing method comprising the steps of:

[0069] (1) First add 1ml of anticoagulant protectant to a 2ml centrifuge tube;

[0070] (2) Then use a scalpel to cut open the blood sinuses of the abalone's gastropod, and place the abalone on ice at an angle of 20-30° so that the blood can flow out more easily;

[0071] (3) After the blood flows out, draw 1ml of blood with a sterile syringe and directly inject it into the centrifuge tube after adding the anticoagulant protective agent;

[0072] (4) adopt the liquid in the hemofilter filtration step (3) in the embodiment 1 to the new 2ml centrifuge tube;

[0073] (5) centrifuge at 18°C ​​and 3000rpm for 10min, and remove the supernatant;

[0074] (6) Finally, resuspend the blood cells treated in step (5) with 1 ml of bacteria-filtered seawater to make abalone hemolymph suspension.

[0075] The anticoagulation protective agent is composed of the following components: 2.55g ...

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Abstract

The invention discloses an abalone blood cell processing method. The method comprises the following steps: adding 1 ml of an anticoagulant protective agent in 2 ml of a centrifuge tube; then using a scalpel for cutting blood sinus of abalone pleopod, inclining the abalone with an angle being 20-30 DEG, and placing the abalone on ice for easily flowing the blood out; after the blood flows out, using an aseptic syringe for extracting 1 ml of blood, and directly injecting the blood into the centrifuge tube added with the anticoagulant protective agent; using a blood filter used for flow cytometerdetection for filtering blood to a new 2 ml of centrifuge tube; performing centrifugation for 10 min at the temperature of 18 DEG C at 3000 rpm, and removing a supernatant; and using 1 ml of bacterial-removal seawater for re-suspending the blood corpuscle to prepare a abalone haemolymph suspension. According to the method, the appropriate blood anticoagulant protective agent and the blood filterare employed, the aggregation time of the blood corpuscle can be effectively prolonged, impurity in the blood can be rapidly filtered, the blood purity is improved, the experiment error is reduced, and the experiment result is more accurate.

Description

technical field [0001] The invention relates to the technical field of processing abalone blood cells, in particular to a method for processing abalone blood cells. Background technique [0002] Due to its rich nutrition and high economic value, abalone has become an important marine aquaculture species in my country. At present, in the field of biological experiments, there is no unified treatment method for abalone blood cells. Abalone blood cells are commonly processed in various ways. In the absence of anticoagulant protection agent, once the abalone blood cells are separated from the body, a large number of agglutinated within a few minutes. Even if some abalone blood cells are added with some anticoagulants, they will die in a large number in a short period of time. This not only requires the experiment to be completed in a short period of time, but also leads to problems such as the experimental results being greatly affected by system errors, increased experimenta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078
CPCC12N5/0634C12N2509/00
Inventor 李明珠杨熙芸孔瑶瑶李圣强时心泽毕秀娟潘世杰姚传伟李浩宇孟晓雪刘明芳阮泽立
Owner LUDONG UNIVERSITY
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