Application of l-thyroxine sodium in preparation of medicine for treating kidney injury caused by depleted uranium
A technology for thyroxine sodium and kidney injury, which is applied in the application field of L-thyroxine sodium, can solve the problems of high nephrotoxicity, water and electrolyte disturbance, and low gastrointestinal absorption rate affected by the pH of catechol compounds
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] Example 1 Cell experiment
[0033] (1) Experimental method: RLE-6TN cells were inoculated in 96-well plates, 5000 cells / well, placed in a constant temperature incubator for 24 hours, and 125, 250, 375, 500, 625 , 750, 875, and 1000 nmol / L L-thyroxine sodium solutions, and set up a normal control group and a control group at the same time, a total of 10 groups. After incubation for 1 h in a constant temperature incubator, 500 umol DU was added to each well except the normal control group. After 24 h of incubation, CCK-8 was added. After 2 h of incubation, the OD value was detected at 450 nm using a microplate reader.
[0034] (2) Results
[0035] Test data:
[0036] Experimental results of L-thyroxine sodium treating DU damaged cells
[0037] NC C 125 250 375 500 625 750 875 1000 1.931 0.517 0.869 0.707 1.028 0.955 1.010 1.074 0.794 0.537 1.484 0.523 0.533 0.738 1.124 0.823 1.006 1.075 0.540 0.624 1.917 0.522 ...
Embodiment 2
[0040] Embodiment 2 Animal experiments
[0041] (1) Materials: Sprague Dawley male rats, body weight 200±20g, euthyrox (provided by Merck KGaA, Darmstadt), DU, ultrapure water.
[0042] (2) Instruments: syringes, gavage needles
[0043] (3) Drug preparation method: make Eumethalol into powder, weigh an appropriate amount, suspend Eumethalone with ultrapure water, and draw the suspension. When using, suspend the solution evenly, and absorb an appropriate amount (in the form of L-thyroid Calculation based on the content of plain sodium), add ultrapure water and mix well, and administer by gavage.
[0044](4) Experimental method: 30 Sprague Dawley male rats (body weight 200±20 g) were randomly divided into 5 groups, 6 rats / group, respectively normal group (NC), control group (C), 20 ug / kg treatment group, 40 ug / kg treatment group, and 80 ug / kg treatment group. The NC group received no treatment and was fed normally; the C group received intraperitoneal injection of DU 5 mg / kg...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More 


