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Method for extracting millipede protein hydrolysates and application thereof

A technology of hydrolyzing protein and extracting methods, applied in the direction of peptide preparation methods, applications, chemical instruments and methods, etc., to achieve the effect of high nutritional value and low cost

Inactive Publication Date: 2018-08-17
FOSHAN HUIMU CHEM TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no relevant report about the hydrolyzed protein of millipede

Method used

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  • Method for extracting millipede protein hydrolysates and application thereof

Examples

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Embodiment 1

[0024] A method for extracting hydrolyzed protein from millipede, comprising the steps of:

[0025] (1) Collect millipede, after cleaning, process with a high-speed tissue grinder, and the speed of the grinder is 12000r / min;

[0026] (2) Add 12.5 times of distilled water to the crushed millipede tissue in step (1), and then add 4% protease to carry out enzymolysis in a 48°C water bath for 6.5 hours. The protease is composed of papain and subtilisin in proportion to 1 :2 composition;

[0027] (3) Put the millipede tissue after enzymolysis in step (2) into an 88°C water bath and heat for 13 minutes to inactivate the enzyme, and filter to obtain an enzymolysis solution;

[0028] (4) The enzymolysis solution in step (3) is first filtered by an ultrafiltration membrane with a molecular weight of 30,000 Daltons, and then filtered and concentrated by an ultrafiltration membrane with a molecular weight of 500 Daltons to obtain a hydrolyzed protein of millipede of concentrate.

Embodiment 2

[0030] A method for extracting hydrolyzed protein from millipede, comprising the steps of:

[0031] (1) Collect millipede, after cleaning, process it with a high-speed tissue grinder, and the speed of the grinder is 10000r / min;

[0032] (2) Add 10 times of distilled water to the crushed millipede tissue in step (1), then add 2% protease and carry out 5h enzymolysis in a 45°C water bath, the protease is composed of papain and subtilisin in proportion 1: 1 composition;

[0033] (3) Put the millipede tissue after enzymatic hydrolysis in step (2) into an 85°C water bath and heat for 10 minutes to inactivate the enzyme, and filter to obtain an enzymatic hydrolysis solution;

[0034] (4) The enzymolysis solution of step (3) is first filtered by an ultrafiltration membrane with a molecular weight of 40,000 Daltons, and then filtered and concentrated by an ultrafiltration membrane with a molecular weight of 500 Daltons to obtain a hydrolyzed protein of millipede of concentrate.

Embodiment 3

[0036] A method for extracting hydrolyzed protein from millipede, comprising the steps of:

[0037] (1) Collect millipede, after cleaning, process it with a high-speed tissue grinder, and the speed of the grinder is 13000r / min;

[0038] (2) Add 15 times of distilled water to the crushed millipede tissue in step (1), then add 6% protease and carry out 8h enzymolysis in a water bath at 50°C, the protease is composed of papain and subtilisin in proportion 1: 3 composition;

[0039] (3) Put the millipede tissue after enzymolysis in step (2) into a 90°C water bath and heat for 15 minutes to inactivate the enzyme, and filter to obtain an enzymolysis solution;

[0040] (4) The enzymolysis solution of step (3) is first filtered by an ultrafiltration membrane with a molecular weight of 50,000 Daltons, and then filtered and concentrated by an ultrafiltration membrane with a molecular weight of 500 Daltons to obtain a hydrolyzed protein of millipede of concentrate.

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Abstract

The invention discloses a method for extracting millipede protein hydrolysates and application thereof, and further provides a formula for preparing foods for pets mink from the millipede protein hydrolysates. The method for preparing the millipede protein hydrolysates approximately includes steps of cleaning fresh millipede; mashing and heating the millipede, adding the millipede into water and enzymes and carrying out enzymatic hydrolysis; carrying out heating to deactivate the enzymes; ultimately carrying out filtering and concentrating to obtain the required millipede protein hydrolysates.The method, the application and the formula have the advantages that physical processes are matched with enzymatic hydrolysis, and accordingly good hydrolysis effects can be realized; the millipede protein hydrolysates with the high quality can be obtained at a low cost, and the high-quality protein foods for the pets mink can be prepared.

Description

technical field [0001] The invention relates to a method for extracting protein, in particular to a method for extracting hydrolyzed protein from millipede. Background technique: [0002] Millipedes are also called millipedes, millipedes, scale rods, etc. Insects belonging to the arthropods, myriapoda, and diplopoda. [0003] Our country has a custom of eating insects, this is because the protein content of insects is higher than that of traditional meat, namely pork, beef, chicken, fish and so on. Moreover, insect protein contains a variety of amino acids needed by the human body, especially lysine, threonine, and methionine that cannot be synthesized by the human body. It is the current trend to study insect protein and prepare it as feed. [0004] There is no related report about the hydrolyzed protein of millipede. Invention content: [0005] The object of the present invention is to provide a method for extracting hydrolyzed protein from millipede to solve the abo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34A23K50/40A23K20/147A23K10/20A23K10/26A23K20/174A23K10/18A23K20/158
CPCA23K10/18A23K10/20A23K10/26A23K20/147A23K20/158A23K20/174A23K50/40C07K1/34C12P21/06
Inventor 不公告发明人
Owner FOSHAN HUIMU CHEM TECH CO LTD
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