Efficient and specific small and compact low-density lipoprotein cholesterol detection kit

Abstract

The invention provides an efficient and specific small and compact low-density lipoprotein cholesterol detection kit. The kit is a liquid dual reagent and consists of a reagent R1 and a reagent R2 according to the volume ratio of 3: 1. A polymer combination including 3-benzyloxy phenyl ether, polyoxyethylene alkyl ether and PEG800 in the efficient and specific small and compact low-density lipoprotein cholesterol detection kit serves as an important composition in the reagent R1 to fully eliminate influences of lipoprotein cholesterol except for sdLDL-C in a to-be-detected sample to sdLDL-C detection well; and moreover, in the reagent R2, by addition of a denaturant Triton X-100, protected sdLDL-C in the to-be-detected sample is released, thus, only sdLDL-C is detected efficiently and specifically, and accuracy of a detecting result is ensured.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to an efficient and specific small and dense low-density lipoprotein cholesterol detection kit. Background technique [0002] Low-density lipoprotein (LDL) is composed of a series of particles with different densities, volumes, and physicochemical properties, and there is obvious heterogeneity. Small dense LDL (sdLDL) is one of the subcomponents of LDL, which plays an important role in the occurrence and development of atherosclerosis (As), ischemic heart disease, and atherosclerosis , is a risk factor for coronary heart disease. In 2002, the National Cholesterol Education Program Adult Treatment Program III used sd LDL as a new risk factor for coronary heart disease. Therefore, more and more attention has been paid to the detection of small and dense low-density lipoprotein cholesterol. [0003] At present, the commonly used method for the detection of small a...

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Problems solved by technology

[0003] At present, the commonly used method for the detection of small and dense low-density lipoprotein cholesterol is the homogeneous method. Using the homogeneous method, the sdLDL to be detected is first protected, and the lipoprotein cholesterol other than sdLDL-C in the sample to be detected is fully detected. The specific detection of sdLDL-C in the sample is performed after the removal of sdLDL-C. This method is easy to operate, so it has been well promoted in recent years, but the difficulty of this method lies in how to choose a suitable surfactant to achieve Selective dissociation of lipoproteins to ensure the accuracy of test results

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