Efficient and specific small and compact low-density lipoprotein cholesterol detection kit

A low-density lipoprotein and detection kit technology, which is applied in the determination/inspection of microorganisms, measuring devices, instruments, etc., can solve difficult problems and achieve the effect of ensuring accuracy

Inactive Publication Date: 2018-08-17
天津中成佳益生物科技有限公司
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  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the commonly used method for the detection of small and dense low-density lipoprotein cholesterol is the homogeneous method. Using the homogeneous method, the sdLDL to be detected is first protected, and the lipoprotein cholesterol other than sdLDL-C in the sample to be detected is fully detected. The s...

Method used

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  • Efficient and specific small and compact low-density lipoprotein cholesterol detection kit
  • Efficient and specific small and compact low-density lipoprotein cholesterol detection kit
  • Efficient and specific small and compact low-density lipoprotein cholesterol detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The specific components of the liquid double reagent of an efficient and specific small and dense low-density lipoprotein cholesterol detection kit are shown in Table 1:

[0028] Table 1 The liquid bismuth of a high-efficiency and specific small and dense low-density lipoprotein cholesterol detection kit

[0029] Reagent Specific Components

[0030]

[0031] 1) Select 2 samples and dilute 5 gradients. At the same time, select a small and dense low-density lipoprotein cholesterol detection kit that has been marketed internationally for comparison. The detection parameters and procedures are shown in Table 2. The detection results and comparison The relative deviations of the contrasting reagents are shown in Table 3.

[0032] Table 2 Detection parameters and procedures

[0033]

[0034] Table 3 Test results and relative deviation from contrast reagents

[0035]

[0036] It can be seen from the results that the relative deviation between the test results of th...

Embodiment 2-12

[0056] Surfactant A, surfactant B, polyethylene glycol in embodiment 2-12 are as shown in table 8, and other reagents are identical with embodiment 1,

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Abstract

The invention provides an efficient and specific small and compact low-density lipoprotein cholesterol detection kit. The kit is a liquid dual reagent and consists of a reagent R1 and a reagent R2 according to the volume ratio of 3: 1. A polymer combination including 3-benzyloxy phenyl ether, polyoxyethylene alkyl ether and PEG800 in the efficient and specific small and compact low-density lipoprotein cholesterol detection kit serves as an important composition in the reagent R1 to fully eliminate influences of lipoprotein cholesterol except for sdLDL-C in a to-be-detected sample to sdLDL-C detection well; and moreover, in the reagent R2, by addition of a denaturant Triton X-100, protected sdLDL-C in the to-be-detected sample is released, thus, only sdLDL-C is detected efficiently and specifically, and accuracy of a detecting result is ensured.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to an efficient and specific small and dense low-density lipoprotein cholesterol detection kit. Background technique [0002] Low-density lipoprotein (LDL) is composed of a series of particles with different densities, volumes, and physicochemical properties, and there is obvious heterogeneity. Small dense LDL (sdLDL) is one of the subcomponents of LDL, which plays an important role in the occurrence and development of atherosclerosis (As), ischemic heart disease, and atherosclerosis , is a risk factor for coronary heart disease. In 2002, the National Cholesterol Education Program Adult Treatment Program III used sd LDL as a new risk factor for coronary heart disease. Therefore, more and more attention has been paid to the detection of small and dense low-density lipoprotein cholesterol. [0003] At present, the commonly used method for the detection of small a...

Claims

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Application Information

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IPC IPC(8): C12Q1/60C12Q1/30C12Q1/44C12Q1/26
CPCC12Q1/26C12Q1/30C12Q1/44C12Q1/60G01N2333/908G01N2333/916G01N2333/918
Inventor 赵新欣
Owner 天津中成佳益生物科技有限公司
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