Method for early detection of cancer by using nematodes

An early detection and nematode technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of low cancer detection rate, high price, time-consuming and labor-intensive

Inactive Publication Date: 2018-08-21
重庆浦洛通生命科技集团有限公司
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Problems solved by technology

[0005] In order to solve the problem that the detection rate of existing cancer is not high, the price is expensive, and time-consuming and laborious, the present invention proposes a new method for detecting cancer in nematodes based on the research on the chemotaxis of nematode smell or olfactory nerve

Method used

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  • Method for early detection of cancer by using nematodes
  • Method for early detection of cancer by using nematodes
  • Method for early detection of cancer by using nematodes

Examples

Experimental program
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Effect test

Embodiment 1

[0081] cancer detection

[0082] a. Breeding of nematodes

[0083] Five to six adults of wild-type nematode N2 were placed in a 6 cm petri dish (NGM medium coated with Escherichia coli), and cultured at 20° C. for 4 days. 300 to 500 adults of the next-generation nematodes were bred.

[0084] b. Analyzing Chemotaxis

[0085] In a 9cm Petri dish press figure 2 4 drops of 0.5ul sodium azide (NaN 3 ).

[0086] Add 1ml of washing buffer to the nematode feeding plate, and collect the floating nematodes with a test tube. Since the nematodes will sink after a little standing, discard the supernatant after the nematodes sink. Then add 1 ml of washing buffer to the test tube, discard the supernatant after the nematode sinks, repeat this process 3 times, and remove Escherichia coli.

[0087] 1 μl of urine samples diluted 10 times with sterile distilled water were dropped into the "+" parts of the 9 cm petri dish.

[0088] c. Place about 100 nematodes in the center of the petri dis...

Embodiment 2

[0093] In this example, although the sediment and solids in the urine were removed by centrifugation and filtration, this treatment had no effect on the chemotaxis of nematodes ( image 3 ).

Embodiment 3

[0095] In this embodiment, the established tumor cell line and the culture medium or storage solution are used as the model of the subject's living body-related substances to carry out the cancer detection experiment.

[0096] 1. Using tumor cell lines to detect cancer

[0097] To detect cancer using culture supernatants of human tumor cell lines, colon cancer (rectal cancer) cells of SE480, COLO201 and COLO205, breast cancer cells of MCF7, and gastric cancer cells of NUGU4, MKN1 and MKN7 were used.

[0098] SW480, COLO201, COLO205 and other cells were obtained from the Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. All cell lines used BPMI1640 medium with 10% FBS (fetal bovine serum), maintained at 37°C, 5% CO 2 Non-fused state under ventilated conditions. The culture medium of the clear layer above the culture medium was used in the experiment. The culture medium after culturing the cells was placed on the assay plate ( ...

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Abstract

The invention provides a method for detection of cancer. The method is characterized in that nematodes with chemotaxis to biomaterials or products thereof from subjects are used as indicators for detection of cancers. The method for detection of cancer in the invention can alleviate the pain of the subjects, is simple to operate and low in cost; and the method is of great use as a novel high-precision cancer detection method applicable to most people.

Description

technical field [0001] The present invention relates to a new type of cancer diagnosis technology. Specifically, the present invention relates to a method for detecting the smell from the test subject's biological material or its products by using the nematode's sense of smell, and diagnosing cancer according to the response of the nematode. Background technique [0002] According to the statistics of cancer in China in 2015, the current incidence of cancer in my country is 285.91 / 100,000, and an average of 6 people are diagnosed with malignant tumors every minute. The national cancer mortality rate is 180.54 / 100,000, and the annual number of cancer deaths reaches 2.7 million. The probability of death due to cancer among Chinese residents is 13%, that is to say, 1 person in every 7-8 people dies from cancer. The number of deaths due to cancer in the world has reached 8 million every year, and it is expected to continue to increase in the future. [0003] The earlier the ca...

Claims

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Application Information

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IPC IPC(8): C12Q1/20
CPCC12Q1/20
Inventor 张军
Owner 重庆浦洛通生命科技集团有限公司
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