Stem cell spheres and preparation method thereof
A stem cell sphere and stem cell technology, applied in the field of tissue engineering, can solve problems such as the limited proportion of stem cells in the stem cell sphere, reduce stem cell differentiation, etc., achieve rapid response to the force of cells, simplify the process of forming stem cell spheres, and avoid asymmetry. The effect of splitting or symmetric differentiation
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Embodiment 1
[0043] The stem cell spheres of this example were prepared by the following method:
[0044] (1) Preparation of liquid crystal materials
[0045] Add 5g of Hydroxypropyl cellulose (Hydroxypropyl cellulose, HPC, MW = 100000) to 30mL of acetone, and then add 4mL, 5.5mL, 7mL, 8.5mL of Octanoyl chloride (OC) dropwise after completely dissolved to prepare four feeds Specific liquid crystal state octanoyl acyl-acryloyl hydroxypropylcellulose ester (OPC-1, OPC-2, OPC-3, OPC-4), the preparation method refers to patent ZL201310185088.4, the steps are as follows:
[0046] After reacting at 55℃ for 4h, add 200mL deionized water to the mixed solution of the reaction, after stirring for 5h, remove the aqueous solution, dissolve the precipitate in acetone, add 200mL deionized water to precipitate the product, repeat the above adding deionized water After the process of stirring, standing, removing water, dissolving the precipitate in acetone for 5 times, dialyzing in deionized water at 25°C for 4...
Embodiment 2
[0080] The stem cell spheres of this example were prepared by the following method:
[0081] (1) Preparation of liquid crystal materials
[0082] According to the following methods, five liquid crystal Cholesterol Tetraglycol Carbonate (CTGC) with different feeding ratios were prepared:
[0083] Cholesteryl chloroformate was added to 20 mL of purified chloroform, and stirred at room temperature until completely dissolved. Add 10 mL of pyridine and 8 mL of tetraethylene glycol to a three-necked flask. Stir at 45°C, add dropwise the completely dissolved cholesteryl chloroformate chloroform solution, heat to 55°C, and react for 12 hours. After cooling to room temperature, dialyze in deionized water for 48 hours. Under the condition of 60℃, -0.05MPa, vacuum drying for 12h to obtain CTGC; wherein the masses of cholesteryl chloroformate added are 2.2g, 3.3g, 4.4g, 5.5g, 6.6g, respectively, corresponding to the prepared CTGC -1, CTGC-2, CTGC-3, CTGC-4, CTGC-5, respectively detect the deg...
Embodiment 3
[0096] The stem cell spheres of this example were prepared by the following method:
[0097] (1) Preparation of liquid crystal materials
[0098] Preparation of 3 kinds of liquid crystal N-phthalylated chitosan (see: Wang Mian, Research on the Liquid Crystal Behavior of Phthalylated Chitosan, 2001, Master's thesis of Xiamen University. Use the room temperature reaction in the article Preparation conditions, the molar ratios of phthalic anhydride and deacetylated chitosan are 26:1, 24:1, and 22:1 respectively. The products obtained are referred to as APHCS-1, APHCS-2, APHCS-3), respectively The degree of substitution of the flexible chain was tested, and APHCS-1 and APHCS-2 with degrees of substitution of 80.3% and 82.1% were retained, respectively.
[0099] The liquid crystal state N-phthalylated chitosan is prepared from phthalic anhydride and chitosan, wherein the weight average molecular weight of chitosan is 720,000-760,000, and the degree of deacetylation of chitosan is 80 ~90...
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