Stem cell sphere and preparation method thereof
A technology of stem cell spheres and stem cells, applied in the field of tissue engineering, can solve the problems of limited proportion of stem cells in stem cell spheres, reduce stem cell differentiation, etc., and achieve the effect of avoiding asymmetric division or symmetric differentiation
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Embodiment 1
[0043] The stem cell spheres of this embodiment were prepared by the following method:
[0044] (1) Preparation of liquid crystal materials
[0045]Add 5g of hydroxypropyl cellulose (Hydroxypropyl cellulose, HPC, MW=100000) to 30mL of acetone, and after complete dissolution, add 4mL, 5.5mL, 7mL, and 8.5mL of octanoyl chloride (Octanoyl chloride, OC) dropwise to prepare four kinds of feeding materials Relative liquid crystal hydroxypropylcellulose ester (octanoyl acyl-acryloyl hydroxypropylcellulose ester, OPC-1, OPC-2, OPC-3, OPC-4), the preparation method refers to the patent ZL201310185088.4, the steps are as follows:
[0046] Under the condition of 55℃, react for 4h, add 200mL deionized water to the reaction mixed solution, stir for 5h, remove the aqueous solution, dissolve the precipitate in acetone, then add 200mL deionized water to precipitate the product, repeat the above adding deionized water , stir, stand still, remove water, dissolve the precipitate in acetone 5 ti...
Embodiment 2
[0080] The stem cell spheres of this embodiment were prepared by the following method:
[0081] (1) Preparation of liquid crystal materials
[0082] According to the following method, five kinds of liquid crystal cholesteryl tetraethylene glycol carbonate (Cholesterol Tetraglycol Carbonate, CTGC) with different charging ratios are prepared:
[0083] Add cholesteryl chloroformate to 20 mL of purified chloroform, stir at room temperature until completely dissolved. Add 10 mL of pyridine and 8 mL of tetraethylene glycol into a three-neck flask, stir at 45°C, add dropwise the completely dissolved cholesterol chloroformate chloroform solution, heat to 55°C, and react for 12 hours. After cooling down to room temperature, it was dialyzed in deionized water for 48 hours. Vacuum drying at 60°C and -0.05MPa for 12 hours yielded CTGC; among them, the masses of cholesteryl chloroformate added were 2.2g, 3.3g, 4.4g, 5.5g, and 6.6g, respectively, corresponding to the prepared CTGC -1, CT...
Embodiment 3
[0096] The stem cell spheres of this embodiment were prepared by the following method:
[0097] (1) Preparation of liquid crystal materials
[0098] Preparation of 3 kinds of liquid crystal N-phthalylated chitosan (for the preparation method, see: Wang Mian, Research on liquid crystal behavior of phthalylated chitosan, 2001, master's degree thesis of Xiamen University. Using the room temperature reaction in the text Preparation conditions, the molar ratios of phthalic anhydride and deacetylated chitosan used are respectively 26:1, 24:1, and 22:1. The products obtained are referred to as APHCS-1, APHCS-2, and APHCS-3), respectively The degree of substitution of the flexible chain was detected, and APHCS-1 and APHCS-2 with a degree of substitution of 80.3% and 82.1% were retained, respectively.
[0099] The above liquid crystal N-phthalylated chitosan is prepared from phthalic anhydride and chitosan, wherein the chitosan has a weight-average molecular weight of 720,000 to 760,0...
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