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Diagnostic kit for antiviral protein MxA and preparation method of diagnostic kit

A diagnostic kit and anti-virus technology, used in biological testing, material inspection products, etc., can solve the problems of complex operation, unsafe effects, and narrow application scope.

Inactive Publication Date: 2018-08-24
安徽金标点生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the existing MxA diagnostic kits are enzyme-linked immunoassays, which have shortcomings such as narrow application range, unsuitable for clinical use, complicated operation, low precision, long time-consuming, low sensitivity, short validity period, high cost, and unsafe effects.

Method used

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  • Diagnostic kit for antiviral protein MxA and preparation method of diagnostic kit
  • Diagnostic kit for antiviral protein MxA and preparation method of diagnostic kit
  • Diagnostic kit for antiviral protein MxA and preparation method of diagnostic kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Take 4ml of CdSe / ZnS quantum dot toluene solution with a concentration of 5mg / ml, centrifuge at 13000 for 10 minutes, discard the supernatant, and obtain a precipitate, add 120mg of polymethyl methacrylate and 80mg of polystearyl maleic anhydride 2ml of chloroform solution, add 5ml of sodium dodecylsulfonate aqueous solution with a concentration of 3mg / ml, mix for 10 minutes, place in an ultrasonic reactor, select a frequency of 25kHz for ultrasonic homogenization for 2 minutes to obtain an emulsion, and rotate at room temperature for 100 Steam for 4 hours, remove chloroform, and centrifuge the remaining liquid at 10,000 rpm for 10 minutes to obtain a precipitate. Wash twice with 5 ml of water for injection at 10,000 rpm for 10 minutes, add 2 ml of 0.2% sodium azide aqueous solution, put it in an ultrasonic reactor, and select the frequency Ultrasound at 45kHz for 1 minute to obtain a suspension of quantum dot fluorescent microspheres with a concentration of 10mg / ml, and...

specific Embodiment 2

[0061] Take 4ml of CdSe / ZnS quantum dot toluene solution with a concentration of 5mg / ml, centrifuge at 13000 for 10 minutes, discard the supernatant, and obtain a precipitate, add 120mg of polymethyl methacrylate and 80mg of polystearyl maleic anhydride 2ml of chloroform solution, add 5ml of sodium dodecylsulfonate aqueous solution with a concentration of 3mg / ml, mix for 20 minutes, place in an ultrasonic reactor, select a frequency of 25kHz for ultrasonic homogenization for 2 minutes to obtain an emulsion, and rotate at room temperature for 100 Steam for 4 hours, remove chloroform, and centrifuge the remaining liquid at 10,000 rpm for 10 minutes to obtain a precipitate, wash it twice with 5ml of water for injection, centrifuge at 10,000 rpm for 10 minutes, add 2ml of 0.2% sodium azide aqueous solution, put it in an ultrasonic reactor, and select the frequency Ultrasound at 45kHz for 2 minutes to obtain a suspension of quantum dot fluorescent microspheres with a concentration o...

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PUM

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Abstract

The invention discloses a diagnostic kit for antiviral protein MxA and a preparation method of the diagnostic kit. The diagnostic kit and the preparation method are characterized in that the diagnostic kit for the antiviral protein MxA is prepared with a quantum dot technique, a microsphere technique, an intermediate-frequency ultrasonic technique, a suspension technique, a sealing technique and alabeling technique, can be clinically used, is simple to operate, high in precision and sensitivity, long in shelf life and safe to act, application range is wide, detection time is shorter, cost islow, and satisfactory effect is realized.

Description

technical field [0001] The invention relates to the field of diagnostic kit preparation, in particular to an antiviral protein MxA diagnostic kit and a preparation method thereof. Background technique [0002] MxA is a 78kD antiviral protein induced by interferon (IFN). It is a specific indicator for distinguishing whether the body is infected by a virus or a bacterial infection. It is also an important criterion for evaluating the therapeutic effect of interferon. Rapid detection of MxA levels in the body is of great significance for disease diagnosis, rational drug use, and evaluation of curative effects. Most of the existing MxA diagnostic kits are enzyme-linked immunoassays, which have shortcomings such as narrow application range, unsuitable for clinical use, complicated operation, low precision, long time-consuming, low sensitivity, short validity period, high cost, and unsafe effects. . Based on years of experience in the development of diagnostic kits, the present ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 张杰孙威
Owner 安徽金标点生物科技有限公司
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