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Method used for preparing nanometer platinum poly gamma-glutamic acid hydrogel from rhamnolipid

A rhamnolipid and glutamic acid technology, applied in the medical field, can solve the problems of poor embedding effect, low encapsulation rate, and uneven distribution of nano-platinum, achieving good encapsulation rate, good effect, and wide application Effect

Active Publication Date: 2018-08-28
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the process of preparing nano-platinum poly-γ-glutamic acid hydrogel in the prior art, when the nano-platinum solution is added to the poly-γ-glutamic acid hydrogel, due to the uneven distribution of nano-platinum, resulting in Poor embedding effect, low encapsulation rate, etc.

Method used

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  • Method used for preparing nanometer platinum poly gamma-glutamic acid hydrogel from rhamnolipid
  • Method used for preparing nanometer platinum poly gamma-glutamic acid hydrogel from rhamnolipid
  • Method used for preparing nanometer platinum poly gamma-glutamic acid hydrogel from rhamnolipid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Effect of 5g / L rhamnolipid on nano-platinum poly-γ-glutamic acid hydrogel

[0029] A kind of method adopting rhamnolipid to prepare nano-platinum poly-γ-glutamic acid hydrogel, the steps are:

[0030] 1) Weigh 0.3g of poly-γ-glutamic acid in a 50mL beaker, dissolve it in 45mL of deionized water, and prepare a poly-γ-glutamic acid solution with a concentration of 0.0067g / mL; add nano-platinum into deionized water, and ultrasonically 10 min, mix well, and prepare a 200 μg / mL nano-platinum solution; dissolve the rhamnolipid in deionized water, and prepare a rhamnolipid solution with a concentration of 5 g / L.

[0031] 2) Add 500 μL of EDC / NHS cross-linking agent to the poly-γ-glutamic acid solution (EDC / NHS cross-linking agent is a cross-linking agent solution with a concentration of 1mol / L prepared by dissolving EDC and NHS in deionized water, The molar ratio of EDC to NHS in water is 1:1), and then add 1.5 mL of nano-platinum solution and 1.5 mL of rhamnolipid ...

Embodiment 2

[0036] Example 2 Effect of 10g / L rhamnolipid on nano-platinum poly-γ-glutamic acid hydrogel

[0037] A kind of method adopting rhamnolipid to prepare nano-platinum poly-γ-glutamic acid hydrogel, the steps are:

[0038] 1) Weigh 0.3g of poly-γ-glutamic acid in a 50mL beaker, dissolve it in 45mL of deionized water, and prepare a poly-γ-glutamic acid solution with a concentration of 0.0067g / mL; add nano-platinum into deionized water, and ultrasonically 10 min, mix evenly, and prepare a 200 μg / mL nano-platinum solution; dissolve the rhamnolipid in deionized water, and prepare a rhamnolipid solution with a concentration of 10 g / L.

[0039] 2) Add 500 μL of EDC / NHS cross-linking agent to the poly-γ-glutamic acid solution (EDC / NHS cross-linking agent is a cross-linking agent solution with a concentration of 1mol / L prepared by dissolving EDC and NHS in deionized water, The molar ratio of EDC to NHS in water is 1:1), and then add 1.5 mL of nano-platinum solution and 1.5 mL of rhamnoli...

Embodiment 3

[0044] Example 3 Effect of 15g / L rhamnolipid on nano-platinum poly-γ-glutamic acid hydrogel

[0045] A kind of method adopting rhamnolipid to prepare nano-platinum poly-γ-glutamic acid hydrogel, the steps are:

[0046] 1) Weigh 0.3g of poly-γ-glutamic acid in a 50mL beaker, dissolve it in 45mL of deionized water, and prepare a poly-γ-glutamic acid solution with a concentration of 0.0067g / mL; add nano-platinum into deionized water, and ultrasonically 10 min, mix evenly, and prepare a 200 μg / mL nano-platinum solution; dissolve the rhamnolipid in deionized water, and prepare a rhamnolipid solution with a concentration of 15 g / L.

[0047] 2) Add 500 μL of EDC / NHS cross-linking agent to the poly-γ-glutamic acid solution (EDC / NHS cross-linking agent is a cross-linking agent solution with a concentration of 1mol / L prepared by dissolving EDC and NHS in deionized water, The molar ratio of EDC to NHS in water is 1:1), and then add 1.5 mL of nano-platinum solution and 1.5 mL of rhamnoli...

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Abstract

The invention discloses a method used for preparing nanometer platinum poly gamma-glutamic acid hydrogel from rhamnolipid. The raw materials comprise rhamnolipid, nanometer platinum, chitosan oligosaccharide, poly gamma-glutamic acid, EDC / NHS crosslinking agent, and glycerol. The nanometer platinum poly gamma-glutamic acid hydrogel can applied in medical products, and especially in wound dressing.According to the method, rhamnolipid is taken as a biosurfactant, and the nanometer platinum polymer drug is obtained under the effect of rhamnolipid; the nanometer platinum poly gamma-glutamic acidhydrogel possesses excellent biocompatibility, is capable of absorbing secretion of wounds, is excellent in antibacterial performance, and is capable of inhibiting growth of bacteria at wounds. Rhamnolipid is taken as the first choice of the surfactant, and drug encapsulation rate is relatively high.

Description

technical field [0001] The invention belongs to the medical field, and relates to a method for preparing a nano-platinum poly-γ-glutamic acid hydrogel, in particular to a method for preparing a nano-platinum poly-γ-glutamic acid hydrogel by using rhamnolipid. Background technique [0002] Biosurfactant is a type of surfactant produced by microorganisms rather than chemically synthesized. It is purified as a product of functional microorganisms. According to structural characteristics and microbial sources, they can be divided into glycolipids, lipopeptides, and polymer surfactants. Similar to synthetic surfactants, their molecular structure is composed of hydrophilic groups and hydrophobic groups. Today, the most studied biosurfactants are those produced by Pseudomonas aeruginosa, a class of rhamnolipids. [0003] As an anionic surfactant, rhamnolipid can reduce the surface activity of water and change the wettability of solids. It has the functions of emulsification, demul...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/24C08J9/28A61L26/00
CPCA61L26/0004A61L26/0019A61L26/0066A61L26/008A61L2300/102A61L2300/404C08J3/075C08J3/24C08J9/28C08J2305/08C08J2477/04C08L77/00
Inventor 杨革梁鑫鑫车程川巩志金李媛刘金锋
Owner QUFU NORMAL UNIV