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A kind of method adopting rhamnolipid to prepare nano-platinum poly-γ-glutamic acid hydrogel

A technology of rhamnolipid and glutamic acid, applied in the medical field, can solve the problems of poor encapsulation effect, uneven distribution of nano-platinum, low encapsulation rate, etc. Effect

Active Publication Date: 2021-02-09
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the process of preparing nano-platinum poly-γ-glutamic acid hydrogel in the prior art, when the nano-platinum solution is added to the poly-γ-glutamic acid hydrogel, due to the uneven distribution of nano-platinum, resulting in Poor embedding effect, low encapsulation rate, etc.

Method used

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  • A kind of method adopting rhamnolipid to prepare nano-platinum poly-γ-glutamic acid hydrogel
  • A kind of method adopting rhamnolipid to prepare nano-platinum poly-γ-glutamic acid hydrogel
  • A kind of method adopting rhamnolipid to prepare nano-platinum poly-γ-glutamic acid hydrogel

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Example 1 Effect of 5g / L rhamnolipid on nano-platinum poly-γ-glutamic acid hydrogel

[0029] A kind of method adopting rhamnolipid to prepare nano-platinum poly-γ-glutamic acid hydrogel, the steps are:

[0030] 1) Weigh 0.3g of poly-γ-glutamic acid in a 50mL beaker, dissolve it in 45mL of deionized water, and prepare a poly-γ-glutamic acid solution with a concentration of 0.0067g / mL; add nano-platinum into deionized water, and ultrasonically 10 min, mix well, and prepare a 200 μg / mL nano-platinum solution; dissolve the rhamnolipid in deionized water, and prepare a rhamnolipid solution with a concentration of 5 g / L.

[0031] 2) Add 500 μL of EDC / NHS cross-linking agent to the poly-γ-glutamic acid solution (EDC / NHS cross-linking agent is a cross-linking agent solution with a concentration of 1mol / L prepared by dissolving EDC and NHS in deionized water, The molar ratio of EDC to NHS in water is 1:1), and then add 1.5 mL of nano-platinum solution and 1.5 mL of rhamnolipid ...

Embodiment 2

[0036] Example 2 Effect of 10g / L rhamnolipid on nano-platinum poly-γ-glutamic acid hydrogel

[0037] A method for preparing nano-platinum poly-γ-glutamic acid hydrogel by using rhamnolipid, the steps are:

[0038] 1) Weigh 0.3g of poly-γ-glutamic acid in a 50mL beaker, dissolve it in 45mL of deionized water, and prepare a poly-γ-glutamic acid solution with a concentration of 0.0067g / mL; add nano-platinum into deionized water, and ultrasonically 10 min, mix evenly, and prepare a 200 μg / mL nano-platinum solution; dissolve the rhamnolipid in deionized water, and prepare a rhamnolipid solution with a concentration of 10 g / L.

[0039] 2) Add 500 μL of EDC / NHS cross-linking agent to the poly-γ-glutamic acid solution (EDC / NHS cross-linking agent is a cross-linking agent solution with a concentration of 1mol / L prepared by dissolving EDC and NHS in deionized water, The molar ratio of EDC to NHS in water is 1:1), and then add 1.5 mL nano-platinum solution and 1.5 mL rhamnolipid solutio...

Embodiment 3

[0044] Example 3 Effect of 15g / L rhamnolipid on nano-platinum poly-γ-glutamic acid hydrogel

[0045] A kind of method adopting rhamnolipid to prepare nano-platinum poly-γ-glutamic acid hydrogel, the steps are:

[0046] 1) Weigh 0.3g of poly-γ-glutamic acid in a 50mL beaker, dissolve it in 45mL of deionized water, and prepare a poly-γ-glutamic acid solution with a concentration of 0.0067g / mL; add nano-platinum into deionized water, and ultrasonically 10 min, mix evenly, and prepare a 200 μg / mL nano-platinum solution; dissolve the rhamnolipid in deionized water, and prepare a rhamnolipid solution with a concentration of 15 g / L.

[0047] 2) Add 500 μL of EDC / NHS cross-linking agent to the poly-γ-glutamic acid solution (EDC / NHS cross-linking agent is a cross-linking agent solution with a concentration of 1mol / L prepared by dissolving EDC and NHS in deionized water, The molar ratio of EDC to NHS in water is 1:1), and then add 1.5 mL of nano-platinum solution and 1.5 mL of rhamnoli...

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Abstract

The invention discloses a method for preparing nano-platinum poly-γ-glutamic acid hydrogel by using rhamnolipid. The raw materials include rhamnolipid, nano-platinum, chitosan oligosaccharide, poly-γ-glutamic acid, EDC / NHS The cross-linking agent, glycerol, and the nano-platinum poly-γ-glutamic acid hydrogel prepared by the present invention can be applied to medical products, especially in wound dressings. The present invention is characterized in that rhamnolipid is used as a biosurfactant, and the nano-platinum polymer drug obtained under the action of rhamnolipid has good biocompatibility and can absorb the exudate from the wound, while It has high antibacterial performance and inhibits the growth of bacteria at the wound. Rhamnolipid is the first choice as the surfactant, and the drug encapsulation rate is high.

Description

technical field [0001] The invention belongs to the medical field, and relates to a method for preparing a nano-platinum poly-γ-glutamic acid hydrogel, in particular to a method for preparing a nano-platinum poly-γ-glutamic acid hydrogel by using rhamnolipid. Background technique [0002] Biosurfactant is a type of surfactant produced by microorganisms rather than chemically synthesized. It is purified as a product of functional microorganisms. According to structural characteristics and microbial sources, they can be divided into glycolipids, lipopeptides, and polymer surfactants. Similar to synthetic surfactants, their molecular structure is composed of hydrophilic groups and hydrophobic groups. Today, the most studied biosurfactants are those produced by Pseudomonas aeruginosa, a class of rhamnolipids. [0003] As an anionic surfactant, rhamnolipid can reduce the surface activity of water and change the wettability of solids. It has the functions of emulsification, demul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08J3/24C08J9/28A61L26/00
CPCA61L26/0004A61L26/0019A61L26/0066A61L26/008A61L2300/102A61L2300/404C08J3/075C08J3/24C08J9/28C08J2305/08C08J2477/04C08L77/00
Inventor 杨革梁鑫鑫车程川巩志金李媛刘金锋
Owner QUFU NORMAL UNIV