Method and device for rapidly identifying reproductive freezing vitrified solution

Abstract

The invention relates to a method and a device for rapidly identifying a reproductive freezing vitrified solution. The method comprises the following steps: freezing a to-be-detected solution for 5-10seconds at the temperature of minus 70 DEG C to minus 90 DEG C, observing the appearance and the flow condition of the frozen solution, and judging whether the solution is the reproductive freezing vitrified solution; if the frozen solution shows a transparent flow condition, then the solution belongs to the reproductive freezing vitrified solution; and if the frozen solution is white and does not flow, then the solution does not belong to the reproductive freezing vitrified solution. Compared with the conventional detection method, the method has the advantages that the operation is simple,the method takes short time, and the detection can be carried out before reproductive freezing. Therefore, the reproductive freezing failure caused by an agent is avoided, and the unnecessary loss isreduced.

Description

technical field [0001] The invention relates to the field of reproductive freezing, in particular to a method and device for rapidly identifying vitrification fluid for reproductive freezing. Background technique [0002] Reproductive cryopreservation refers to the technology of stopping metabolism of germ cells and embryos at -196°C through special protection measures and cooling procedures, and recovering their metabolic capacity after warming up. With the continuous development of in vitro fertilization-embryo transfer and its derivative technologies, reproductive cryopreservation technology involving germ cells and embryos has become an indispensable part in the field of human assisted reproduction or animal breeding. [0003] Currently, conventional reproductive freezing techniques include slow freezing and vitrification. [0004] Among them, the slow freezing method is the main method for cryopreservation of most mammalian reproduction. In this method, a lower concen...

AI Technical Summary

Problems solved by technology

[0010] 1. The discovery process takes a long time, often after freezing and preservation, adverse consequences appear when the patient needs to thaw germ cells or embryo transfer, and the operator's attention may not be aroused until at least one month later

[0011] 2. When the problem is discovered, the number of affected germ cells or embryos is often already large, and a large number of embryos will die, which will affect the interests of patients on a large scale

[0012] 3. It is not easy to master and operate, and requires very high skills for the operator. Only experienced operators can better grasp this method, which is not conducive to popularization and implementation

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