41results about How to "Large amount of detection" patented technology

The invention discloses a fluorescent quantitative RT-PCR detection reagent for a bean pod mottle virus, a preparation method thereof and application of the fluorescent quantitative RT-PCR detection reagent for the bean pod mottle virus. The fluorescent quantitative RT-PCR detection reagent for the bean pod mottle virus is synthesized through elaborate design by selecting conservative CP encodinggene fragments between various strains taking special bean pod mottle virus as target sequences, and comprises a pair of special primers and a special fluorescent probe, wherein the length of an amplified target fragment is 98 bp; and the sequences of the primers and the probe are as follows: the sequences of the primers are BPMV TF: 5'-ttgatggcacaggaggaaatt-3' and BPMV TR: 5'-acatgcattgctgtagcttgag-3', and the sequence of the probe is 5'-FAM-tgtcttctagtgcaagtga-MGB-3'. The fluorescent quantitative RT-PCR detection reagent for the bean pod mottle virus overcomes the defects of time consumption, easy pollution, requirement of electrophoretic detection after amplification and so on. of general RT-PCR, can quickly and accurately perform qualitative and quantitative detection on BPMV in a sample, and has the advantages of simplicity, easy operation, directviewing result, high sensitivity, good repeatability and so on.

The invention relates to a method and a device for rapidly identifying a reproductive freezing vitrified solution. The method comprises the following steps: freezing a to-be-detected solution for 5-10seconds at the temperature of minus 70 DEG C to minus 90 DEG C, observing the appearance and the flow condition of the frozen solution, and judging whether the solution is the reproductive freezing vitrified solution; if the frozen solution shows a transparent flow condition, then the solution belongs to the reproductive freezing vitrified solution; and if the frozen solution is white and does not flow, then the solution does not belong to the reproductive freezing vitrified solution. Compared with the conventional detection method, the method has the advantages that the operation is simple,the method takes short time, and the detection can be carried out before reproductive freezing. Therefore, the reproductive freezing failure caused by an agent is avoided, and the unnecessary loss isreduced.

The invention provides a fault diagnosis method of an S4R serial-connection type sequence switch shunt regulator. The method includes following steps: analyzing the operating state of the S4R serial-connection type sequence switch shunt regulator in a no-fault condition; obtaining fault modes of a single-stage shunt regulating circuit in the fault condition of one or more switches through analysis; selecting detection points as solar cell sub-array voltages corresponding to multiple channels of shunt regulation circuits, and selecting a sampling frequency; selecting a sampling data time lengthto cover a complete switching cycle of the solar cell sub-array voltage; sampling the solar cell sub-array voltage corresponding to each stage of shunt regulation circuit, and sampling a storage battery pack voltage Vbat; searching a regulation stage; performing fault diagnosis according to the found regulation state; and continuing next round of sampling and diagnosis. By adopting the technicalscheme of the method, normal operation of the shunt regulator is not affected, the detection omission rate of faults is low, the fault detection rate is high, accurate positioning of the faults is realized, and the diagnosis speed is fast.