41results about How to "Large amount of detection" patented technology

Significantly higher yield and better resolution in pI gels are obtained by creating traps having two or more layers of gel containing closely stepped immobilized pH buffers. Proteins move from a pH at which they are negatively charged towards an anode at which they are positively charged. Discrete regions containing immobilized pH buffers trap the proteins when the immobilized buffer pH and the protein pI are approximately the same. The protein is trapped within the second layer and not on the surface of or interface of the second layer. Significantly higher yields with better resolution can be obtained through the use of layered sample application gels prior to isoelectric focusing. Layered plugs are prepared with a range of immobilized pH buffers ranging, for example, over 2 pH units, with steps of 0.05 or 0.1 pH units. An array of multilayered plugs wherein each plug has different pH increments is also provided. The array can be used to isolate and trap a variety of proteins having different isoelectric pHs during a single run. Another embodiment provides plugs having at least three layers; a gate layer, a trap layer, and an exit layer. Another embodiment includes adding a carrier ampholytes to running buffers and or adding thiol containing reducing agents to reduce current and improve resolution and collection efficiency.

The invention discloses a method for rapidly detecting ion concentration in sweat. Before usage, deionized water is used for cleaning a sweat adsorption layer, and a sweat removing layer is placed ina sweat collection box; before sporting, the surface of skin is covered with the sweat adsorption layer, and after skin perspiration, sweat is absorbed and collected; when a sweat sensor and the sweatadsorption layer are contacted, ion detection is carried out on the sweat of the sweat adsorption layer by the sweat sensor, the sweat is attached on the sweat sensor, a rotating shaft drives the sweat sensor to continuously rotate, and when the sweat sensor is rotated to contact with the sweat removing layer, and the sweat removing layer is capable of absorbing the sweat attached on the sweat sensor. During sporting, detection is carried out on the sweat generated by a sporter in a real-time mode, motion adjusting and electrolyte supplement are timely prompted to the sporter, and body damagedue to body electrolyte loss by copious sweating can be avoided for the sporter.

The invention discloses a man-machine interaction ground system based on a laser radar. The man-machine interaction ground system comprises at least two laser radar detection devices, a control host and a display screen. The at least two laser radar detection devices are connected to the control host. The display screen is an LED display screen installed on the ground or a projector such that display content is projected to the ground. Radial laser scanning surfaces, parallel to the ground, are formed on the laser radar detection devices and used for detecting touch motion on the scanning surfaces and positioning information on polar coordinates of touch points. The control host is used for calculating spatial position of the touch points based on information on polar coordinates of the touch points and the corresponding laser radar detection devices. By adoption of the above mode, the problem of existing laser radar touch technology such as limited detection capacity is solved and the size of a display area is flexibly suited.

The invention discloses a laser radar based man-machine interaction system. The system comprises at least two laser radar detection apparatuses, a control host and a display screen, wherein the at least two laser radar detection apparatuses are connected with the control host; the laser radar detection apparatuses form a radial laser scanning surface in front of the display screen, detect a touch action on the scanning surface and position polar coordinate information of a touch point; and the control host calculates a spatial position of the touch point according to the polar coordinate information of the touch point and spatial position information of the corresponding laser radar detection apparatus. Therefore, the system can solve the problem of limited detection quantity in an existing laser radar touch technology.

The invention discloses a puffer fish ingredient fluorescence quantitative PCR detection reagent and a preparation method and application thereof, in the technical scheme, relatively conservative cytochrome B gene fragments of different puffer fishes are selected to be the target sequences, after elaborately design and synthesis, a pair of specific primer and a specific fluorescent probe are contained, the length of the target segment is enlarged to 102bp, the sequences of the primer and the probe are respectively as follows: primer: Takifugu TF:5'-TCTTCACGAAACAGGCTCCAAC-3', Takifugu TR: 5'-GTGAAGCCCAGGAGGTCTTTGTA-3'; and probe: 5'-FAM-CGCAGACAAAATCCCATTCCACCCATA-TAMRA-3'. The invention is the fluorescence quantitative PCR detection reagent with high specificity, strong sensibility and rapid detection to the puffer fish ingredient mixed with minced fish products; and the method of the invention has reasonable design and accurate definiteness.

The present invention discloses a laser radar based stage interaction system. The system comprises at least two laser radar detection devices, a control host and a stage screen, wherein the at least two laser radar detection devices are connected to the control host; the laser radar detection devices form a radial laser scanning surface in front of performers on a stage, detect touch actions on the scanning surface and further locate polar coordinate information of one or more touch points; and the control host calculates the spatial position of the touch point(s) according to the polar coordinate information of the touch point(s) and spatial position information of the corresponding laser radar detection devices. Therefore, by using the system provided by the present invention, real-time interaction between the performers on the stage and the stage screen can be achieved; the problem of decreased detection accuracy of a remote end can be solved; and the detectable amount is effectively enlarged.

The invention provides a fluorescence quantitative RT-PCR detecting reagent for a wheat streak mosaic and a preparation method and applications thereof; the fluorescence quantitative RT-PCR detecting reagent for the wheat streak mosaic is finely designed and composed by taking the specific and more conservative CP coding gene section between each strain of the wheat streak mosaic as a target sequence; the fluorescence quantitative RT-PCR detecting reagent for the wheat streak mosaic comprises a pair of specific primers and a specific fluorescence probe; the length of a target augmentation section is 63bp; the sequences of the primers and the probe are as follows: the primer WSMV TF is 5'- agctatttgcacaggcacga-3'; the primer WSMV TR is 5'- ctgcatcatgacgtgagttgtc-3' and the probe is 5'- FAM-tgagtgcgggtactaatga-MGB-3'. The invention overcomes the defects of time waste, easy pollution and needing electrophoresis detection after augmentation of the normal RT-PCR, can carry out quick and accurate qualitative and quantitative detection on the WSMV in samples and has the advantages of simple and easy operation, intuitionistic result, high sensibility, good repetitiveness, and the like.

The invention relates to a nucleic acid aptamer, a complementary sequence and a nondiagnostic detection method for detecting hemolytic streptococcus, and belongs to the fields of clinical examination and food detection. The nucleic acid aptamer which is connected with an FAM fluorescence signal and is in specific combination with the hemolytic streptococcus, and an oligonucleotide sequence which is complementary with the aptamer are designed to establish a nucleic acid aptamer biosensor detection method for detecting the hemolytic streptococcus on the basis of colloidal gold quenching fluorescence. The nucleic acid aptamer, the complementary sequence and the detection method are mainly used for quickly detecting the hemolytic streptococcus, and have the advantages of strong specificity, high sensitivity, high detection efficiency, simplicity in operation and high safety.

The invention discloses an island detection method for a droop control grid-connected inverter. The method is implemented specifically according to the following steps: 1, a three-phase grid-connectedinverter main circuit system is built; 2, output active power and reactive power of the three-phase grid-connected inverter are calculated; 3, an improved P-f droop controller with disturbance k(f-f<g>)/s added is utilized to calculate reference output of voltage amplitude and phase; 4, difference between the reference voltage and phase and actual voltage and phase is calculated to obtain an error voltage value as reference input of an outer ring regulator, output of the regulator is reference input of inner ring current, difference between the reference input of the inner ring current and actual current is calculated to obtain an error current value as input of an inner ring regulator, and finally, an output modulation signal is obtained; and 5,the value of |d[delta][theta]/dt|=|k(f-f<g>)| is monitored in real time to perform island judgment. According to the island detection method for a droop control grid-connected inverter, on the basis of a traditional island monitoring droop improvement method, integration is added for the disturbance part, the amplitude of |d[delta][theta]/dt| is increased and stable amplitude is maintained, thereby avoiding island misjudgment.

The invention provides a microfluidic detection device which comprises a rack, a centrifugal module, an automatic card loading module, an automatic card unloading module, a card loading station, a card unloading station, a waiting station corresponding to the card loading station, and a guide channel arranged between the waiting station and the card loading station, the automatic card feeding module comprises a card pushing piece and a first driving mechanism, the output end of the first driving mechanism is connected with the card pushing piece, and the card pushing piece is in surface contact with the micro-fluidic chip in the card pushing process, so that the stressed area is large, and the card can be stably and accurately pushed; the pushing force is large, so that in the process of stably pushing the micro-fluidic chip, the reaction cavity can be pushed to be close to the puncture structure, and automatic puncture is realized.

The invention relates to a method and a device for rapidly identifying a reproductive freezing vitrified solution. The method comprises the following steps: freezing a to-be-detected solution for 5-10seconds at the temperature of minus 70 DEG C to minus 90 DEG C, observing the appearance and the flow condition of the frozen solution, and judging whether the solution is the reproductive freezing vitrified solution; if the frozen solution shows a transparent flow condition, then the solution belongs to the reproductive freezing vitrified solution; and if the frozen solution is white and does not flow, then the solution does not belong to the reproductive freezing vitrified solution. Compared with the conventional detection method, the method has the advantages that the operation is simple,the method takes short time, and the detection can be carried out before reproductive freezing. Therefore, the reproductive freezing failure caused by an agent is avoided, and the unnecessary loss isreduced.

The invention relates to the field of fabricated buildings, and particularly discloses a fabricated building for public health emergencies. The fabricated building comprises a plurality of lower-layer units, upper-layer units, a plurality of upper-layer frames and a plurality of lower-layer frames which are in fan shapes, the upper layer units are arranged in the upper mounting frames, the lower layer units are arranged in the lower mounting frames, windows are formed in the inner side end and the outer side end of the lower layer unit, a door body is further arranged at the outer side end of the lower layer units, stair grooves are formed in the bottom of the upper layer units and the top of the lower layer units, the stair grooves are close to the outer side ends of the upper layer units and the lower layer units, stairs are arranged in the upper layer units and the lower layer units, the lower ends of the stairs are connected with the lower layer units, and the upper ends of the stairs are connected with the upper layer units. The fabricated building aims to solve the technical problems that the existing fabricated building has few partitions and the building space cannot be effectively utilized.

The invention provides a fault diagnosis method of an S4R serial-connection type sequence switch shunt regulator. The method includes following steps: analyzing the operating state of the S4R serial-connection type sequence switch shunt regulator in a no-fault condition; obtaining fault modes of a single-stage shunt regulating circuit in the fault condition of one or more switches through analysis; selecting detection points as solar cell sub-array voltages corresponding to multiple channels of shunt regulation circuits, and selecting a sampling frequency; selecting a sampling data time lengthto cover a complete switching cycle of the solar cell sub-array voltage; sampling the solar cell sub-array voltage corresponding to each stage of shunt regulation circuit, and sampling a storage battery pack voltage Vbat; searching a regulation stage; performing fault diagnosis according to the found regulation state; and continuing next round of sampling and diagnosis. By adopting the technicalscheme of the method, normal operation of the shunt regulator is not affected, the detection omission rate of faults is low, the fault detection rate is high, accurate positioning of the faults is realized, and the diagnosis speed is fast.

The invention relates to a method for improving mass detection of chemical components of a crude tobacco package in tobacco leaf tobacco industries, and in particularly relates to a method for improving the detection efficiency and representativeness of the chemical components of the crude tobacco package. The method includes the following steps: sampling multiple points of tobacco leaves in a to-be-tested crude tobacco package for four or more than times, and mixing the tobacco leaves obtained by the four or more than times of sampling for detecting; spreading flatly the obtained tobacco leaves on a detection conveyor belt, starting the detection conveyor belt, conveying the tobacco leaves by the detection conveyor belt to pass through a light spot irradiation detection area of an on-line near infrared spectroscopy, processing obtained near infrared spectrum of each chemical component by detecting measuring software for detecting, and after the processing by the detecting measuring software, outputting detection numerical values of all the chemical components, and recording the numerical values of all the chemical components; performing numerical optimization on the numerical values obtained by the on-line near infrared spectroscopy to obtain the chemical component values of the whole crude tobacco package. The fast detection function of the chemical components of the crude tobacco hemp package can be realized, and the method has the advantage of high efficiency, complete detection and representative detection values.