41results about How to "Large amount of detection" patented technology

The invention relates to a fluorescent polymerase chain reaction (PCR) primer, a probe and a method for detecting the whole group of hemolytic streptococcus, belonging to the field of inspection and quarantine. In the primer, hemolysin S of A, C and G group hemolytic streptococcus is used as a target gene site to design a fluorescent PCR primer and a probe and establish a fluorescent PCR detection method for the whole group of the hemolytic streptococcus. The fluorescent PCR primer, the probe and the method are mainly used for detecting the hemolytic streptococcus.

The invention discloses a fluorescent quantitative RT-PCR detection reagent for a bean pod mottle virus, a preparation method thereof and application of the fluorescent quantitative RT-PCR detection reagent for the bean pod mottle virus. The fluorescent quantitative RT-PCR detection reagent for the bean pod mottle virus is synthesized through elaborate design by selecting conservative CP encodinggene fragments between various strains taking special bean pod mottle virus as target sequences, and comprises a pair of special primers and a special fluorescent probe, wherein the length of an amplified target fragment is 98 bp; and the sequences of the primers and the probe are as follows: the sequences of the primers are BPMV TF: 5'-ttgatggcacaggaggaaatt-3' and BPMV TR: 5'-acatgcattgctgtagcttgag-3', and the sequence of the probe is 5'-FAM-tgtcttctagtgcaagtga-MGB-3'. The fluorescent quantitative RT-PCR detection reagent for the bean pod mottle virus overcomes the defects of time consumption, easy pollution, requirement of electrophoretic detection after amplification and so on. of general RT-PCR, can quickly and accurately perform qualitative and quantitative detection on BPMV in a sample, and has the advantages of simplicity, easy operation, directviewing result, high sensitivity, good repeatability and so on.

The invention relates to a method for estimating the number of vehicles at a road segment based on a travel time distribution rule. The method comprises the steps: (1), pre-building of a travel time distribution model of different turning directions and time periods: extracting the travel time samples of a road segment m at the same date in one historical cycle for classification to obtain a travel time sample set, and building the travel time distribution model of different turning directions and time periods; (2), the real-time estimation of the number of online vehicles at the road segment:collecting the data of identity detection equipment at a downstream intersection of the same road segment m in real time; calculating the moment when a vehicle enters the road segment based on the travel time, which is detected by the estimation of the above model and is employed by the vehicle for passing through the road segment m, through the moment when the vehicle leaves the road segment m and the estimated travel time; judging whether the vehicle is located on the road segment m at the moment t or not: adding one to the number of vehicles if the vehicle is located on the road segment mat the moment t, or else performing no recording, and finally obtaining the number of vehicles on the road segment m at the moment t through accumulation. The method is good in implementation performance, is high in efficiency, is low in cost, and can be widely used for the estimation of the number of vehicles at road segment.

The invention relates to a method and a device for rapidly identifying a reproductive freezing vitrified solution. The method comprises the following steps: freezing a to-be-detected solution for 5-10seconds at the temperature of minus 70 DEG C to minus 90 DEG C, observing the appearance and the flow condition of the frozen solution, and judging whether the solution is the reproductive freezing vitrified solution; if the frozen solution shows a transparent flow condition, then the solution belongs to the reproductive freezing vitrified solution; and if the frozen solution is white and does not flow, then the solution does not belong to the reproductive freezing vitrified solution. Compared with the conventional detection method, the method has the advantages that the operation is simple,the method takes short time, and the detection can be carried out before reproductive freezing. Therefore, the reproductive freezing failure caused by an agent is avoided, and the unnecessary loss isreduced.

The invention provides a fault diagnosis method of an S4R serial-connection type sequence switch shunt regulator. The method includes following steps: analyzing the operating state of the S4R serial-connection type sequence switch shunt regulator in a no-fault condition; obtaining fault modes of a single-stage shunt regulating circuit in the fault condition of one or more switches through analysis; selecting detection points as solar cell sub-array voltages corresponding to multiple channels of shunt regulation circuits, and selecting a sampling frequency; selecting a sampling data time lengthto cover a complete switching cycle of the solar cell sub-array voltage; sampling the solar cell sub-array voltage corresponding to each stage of shunt regulation circuit, and sampling a storage battery pack voltage Vbat; searching a regulation stage; performing fault diagnosis according to the found regulation state; and continuing next round of sampling and diagnosis. By adopting the technicalscheme of the method, normal operation of the shunt regulator is not affected, the detection omission rate of faults is low, the fault detection rate is high, accurate positioning of the faults is realized, and the diagnosis speed is fast.

The invention belongs to the technical field of chemical analysis and detection and discloses a method for rapidly determining the iron content of slag. The method comprises the following steps: grinding slag powder for 30-300s, weighing the ground slag powder, arranging the ground slag in a tray, arranging a magnet at the bottom of the tray, flushing the slag powder for 5-10min with water, then arranging iron in the tray in a drying box, drying the iron at the temperature of 105-200 DEG C to constant weight, weighing the mass of the iron and computing the iron content of the slag. A device used in the method is simple and easy to operate, the detection cost is low, the determination speed is high, the detection quantity of samples to be detected is larger, sampling is more typical, errors caused by uneven sampling are avoided, the detection result is relatively accurate, a new choice is added for the determination field, and the method has wide application prospects.

The invention discloses a pythium splendens braun fluorescence quantitative PCR detection reagent, a detection kit and an application; the detection reagent comprises a pair of specific primers with sequences as shown in SEQ ID NO: 1 and SEQ ID NO: 2, and a specific fluorescence probe with a sequence as shown in SEQ ID NO: 3; an amplification target fragment has a length of 94 bp, and the nucleotide sequence of the amplification target fragment is shown in SEQ ID NO: 4; the detection kit comprises components of a CTAB extract, a PCR buffer containing the primers and the probe, TaqDNA polymerase, positive control liquid, and quantitative standard liquid; the detection method comprises the extraction of total RNA and the fluorescence PCR reaction; detection performed by using the pythium splendens braun fluorescence quantitative PCR detection reagent overcomes disadvantages of time consumption, easy pollution, and electrophoresis detection necessity after amplification of routine PCR, can realize rapid and accurate qualitative and quantitative detection of pythium splendens braun in a sample, and has the advantages of simplicity, easy operations, intuitive results, high sensitivity, good repeatability, and the like.

The present disclosure relates to a method for screening non-integrating attenuated Listeria strains that highly express foreign proteins. Specifically, the present disclosure relates to a method for detecting the amount of exogenous protein expressed by non-integrated attenuated Listeria, a method for screening non-integrated attenuated Listeria, and a method for screening the non-integrated attenuated Listeria. Non-integrating attenuated Listeria. The method adopted in the present disclosure has the advantages of short time consumption, simple operation, and large sample detection amount, and can meet the requirement of fast and large-scale primary screening of the produced samples while simultaneously producing large batches of samples. Even if there are obvious expression differences among non-integrating Listeria strains, the method of the present disclosure can still achieve rapid and mass screening of non-integrating Listeria, and then obtain the Listeria strain with the best expression effect, which is non-integrating Listeria Vaccine preparation is facilitated.