Method for detecting cadmium ion pollution in water by blocking ELISA (Enzyme-Linked Immunosorbent Assay) method
A cadmium ion detection technology, applied in the field of immunological detection, can solve the problems of small sample screening amount, inability to operate on site, cumbersome and time-consuming, etc., and achieve the effects of high accuracy, strong timeliness, and easy operation.
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[0029] The present invention will be further described below in conjunction with specific embodiments.
[0030] A kind of cadmium ion pollution blocking ELISA detection method in water sample, can carry out Cd first 2+ Preparation of Chelated Complete Antigen and Anti-Cd 2+ - Preparation of EDTA monoclonal antibody, and then specific experiments.
[0031] 1) Cd 2+ Preparation of chelated complete antigen:
[0032] Weigh 10mg ITCBE and dissolve it in 1mL DMSO to form a metal chelating agent solution; weigh 4.2mg CdCl 2 Dissolve in 1 mL of HEPES buffer (10mM / L) pH 8.0 to form Cd 2+ solution; the metal chelating agent solution and Cd 2+ The solution was mixed and the pH value was adjusted to 7.0 with NaOH, and then placed on a shaker at room temperature for 12 hours to form Cd 2+ -ITCBE chelates haptens. 20mg BSA and OVA are dissolved in the HEPES damping fluid (10mM / L) of 1mL pH8.0 respectively to form the carrier protein solution of 20mg / mL; Cd 2+ -ITCBE chelate hapten ...
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