Method for detecting cadmium ion pollution in water by blocking ELISA (Enzyme-Linked Immunosorbent Assay) method

A cadmium ion detection technology, applied in the field of immunological detection, can solve the problems of small sample screening amount, inability to operate on site, cumbersome and time-consuming, etc., and achieve the effects of high accuracy, strong timeliness, and easy operation.

Inactive Publication Date: 2014-11-19
河南华牧生物科技有限公司
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Problems solved by technology

Physical and chemical testing is the current Cd 2+ The main method of pollution detection has high sensitivity and accurate results. However, due to the defects of expensive equipment, skilled professionals, cumbersome and time-consuming, long cycle, high cost, inability to operate on-site, and small sample screening volume, its application is restricted. restricted

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  • Method for detecting cadmium ion pollution in water by blocking ELISA (Enzyme-Linked Immunosorbent Assay) method

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with specific embodiments.

[0030] A kind of cadmium ion pollution blocking ELISA detection method in water sample, can carry out Cd first 2+ Preparation of Chelated Complete Antigen and Anti-Cd 2+ - Preparation of EDTA monoclonal antibody, and then specific experiments.

[0031] 1) Cd 2+ Preparation of chelated complete antigen:

[0032] Weigh 10mg ITCBE and dissolve it in 1mL DMSO to form a metal chelating agent solution; weigh 4.2mg CdCl 2 Dissolve in 1 mL of HEPES buffer (10mM / L) pH 8.0 to form Cd 2+ solution; the metal chelating agent solution and Cd 2+ The solution was mixed and the pH value was adjusted to 7.0 with NaOH, and then placed on a shaker at room temperature for 12 hours to form Cd 2+ -ITCBE chelates haptens. 20mg BSA and OVA are dissolved in the HEPES damping fluid (10mM / L) of 1mL pH8.0 respectively to form the carrier protein solution of 20mg / mL; Cd 2+ -ITCBE chelate hapten ...

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Abstract

The invention belongs to the technical field of immunological detection, mainly relates to immunological quantitative determination of pollution of heavy metal cadmium ion in water, and more specifically relates to a method for detecting cadmium ion pollution in water by a blocking ELISA (Enzyme-Linked Immunosorbent Assay) method. The method is characterized in that Cd<2+> and excessive EDTA are chelated to form a Cd<2+>-EDTA chelate compound; the Cd<2+>-EDTA chelate compound in water and Cd<2+>-ITCBE-OVA coated on an enzyme label plate competes an antigen-binding site of a Cd<2+>-EDTA monoclonal antibody, HRP-labeled goat anti mouse IgG and a TMB substrate are added, after coloration is terminated, A450nm value is read by an ELIASA, by contrasting with a standard curve, the Cd<2+> concentration in water can be obtained. According to the invention, the treatment method of a water sample to be detected is sample, a large-scale apparatus is not required during the detection process, the operation is simple and rapid, sample detection amount is large, timeliness is strong, detection cost is low, popularization is convenient, detection result accuracy is high, repeatability is good, and the detection range is 5.0-512mum g / L.

Description

technical field [0001] The invention belongs to the technical field of immunological detection, and mainly relates to immunological quantitative detection of heavy metal cadmium ion pollution in water samples, in particular to a blocking ELISA detection method for cadmium ion pollution in water samples. Background technique [0002] With the development of industry, heavy metal cadmium (cadmium, Cd) has become one of the public hazards of environmental pollution. Cd in the environment is mainly in the form of ions (Cd 2+ ) exists, and has toxic effects on the kidneys, bones, lungs, and cardiovascular system, as well as carcinogenic, teratogenic, and mutagenic effects on the human body. Therefore, Cd 2+ Pollution detection technology has become one of the research hotspots of scholars at home and abroad. [0003] Currently Cd 2+ There are mainly two types of methods for contamination detection: traditional physical and chemical detection and immunological detection. Phys...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18
Inventor 李生涛刘耀东丁文敏齐子鑫王新炎
Owner 河南华牧生物科技有限公司
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