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Cordyceps ninchukispora strain and cultivation method thereof

A technology of nunchaku and cordyceps, applied in cultivation, plant cultivation, mushroom cultivation and other directions, can solve the problems of high price, destruction of wild resources, etc., and achieve the effects of low cost, simple cultivation method, and simple and easy operation for substrate preparation.

Inactive Publication Date: 2018-09-04
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cordyceps sinensis is an important medicinal fungus. The research and utilization of Cordyceps sinensis in my country has a long history. It cannot be artificially cultivated, causing serious damage to wild resources and expensive prices.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0036] Implementation case 1: With the ratio of 50% polished rice and 50% corn kernels as the cultivation material, the domestication and cultivation of Nunchapeus sinensis comprises the following steps:

[0037] (1) Formula for use: 300g potatoes, 30g glucose, 2g peptone, 0.5g potassium dihydrogen phosphate, 1g magnesium sulfate, vitamin B1-3 tablets. Liquid fermentation was carried out in a shaker at 28°C for 4 days to produce liquid strains.

[0038] (2) Use 8g of polished rice and 8g of corn kernels, use 240ml tissue culture bottles as containers, and fill each bottle with 16g.

[0039] (3) The nutrient solution was prepared by mixing the following mass percentages: magnesium sulfate 0.2%, potassium dihydrogen phosphate 0.3%, vitamin B1-2 tablets / L.

[0040] (4) Add 24ml of nutrient solution to each bottle.

[0041] (5) Sterilize the tissue culture bottle filled with the cultivation material, and use high-pressure steam at a temperature of 121°C for 60 minutes.

[0042]...

Embodiment example 2

[0048] Implementation example 2: With the ratio of 50% corn kernels and 50% wheat as the cultivation material, the domestication and cultivation of Nunchaku Spores comprises the following steps:

[0049] (1) Formula for use: 300g potatoes, 30g glucose, 2g peptone, 0.5g potassium dihydrogen phosphate, 1g magnesium sulfate, vitamin B1-3 tablets. Liquid fermentation was carried out in a shaker at 28°C for 4 days to produce liquid strains.

[0050] (2) Use the ratio of 8g corn kernels and 8g wheat, and use 240ml tissue culture bottles as containers, and fill each bottle with 16g.

[0051] (3) The nutrient solution was prepared by mixing the following mass percentages: magnesium sulfate 0.2%, potassium dihydrogen phosphate 0.3%, vitamin B1-2 tablets / L.

[0052] (4) Add 24ml of nutrient solution to each bottle.

[0053] (5) Sterilize the tissue culture bottle filled with the cultivation material, and use high-pressure steam at a temperature of 121°C for 60 minutes.

[0054] (6) A...

Embodiment example 3

[0060]Implementation example 3: With the ratio of 50% polished rice and 50% wheat as the cultivation material, the domestication and cultivation of Nunchaku Spores comprises the following steps:

[0061] (1) Formula for use: 300g potatoes, 30g glucose, 2g peptone, 0.5g potassium dihydrogen phosphate, 1g magnesium sulfate, vitamin B1-3 tablets. Liquid fermentation was carried out in a shaker at 28°C for 4 days to produce liquid strains.

[0062] (2) Use 8g of polished rice and 8g of wheat, use 240ml tissue culture bottles as containers, and fill each bottle with 16g of material.

[0063] (3) The nutrient solution was prepared by mixing the following mass percentages: magnesium sulfate 0.2%, potassium dihydrogen phosphate 0.3%, vitamin B1-2 tablets / L.

[0064] (4) Add 24ml of nutrient solution to each bottle.

[0065] (5) Sterilize the tissue culture bottle filled with the cultivation material, and use high-pressure steam at a temperature of 121°C for 60 minutes.

[0066] (6)...

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PUM

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Abstract

The invention discloses a cordyceps ninchukispora strain and a cultivation method thereof. The cordyceps ninchukispora (C.H. Su and H.H. Wang) G.H. Sung, J.M. Sung, Hywel-Jones and Spatafora is preserved in the China General Microbiological Culture Collection Center (CGMCC) and the accession number is CGMCC No. 15089. The domestication cultivation method comprises the steps of fermentation of theliquid fungus, preparation of a cultivation medium, bud pressing on a mycelium culture medium, fruiting and the like. According to the invention, the cordyceps ninchukispora strain is domesticated, and the novel strain and the artificial domestication cultivation method are provided.

Description

technical field [0001] The invention relates to a new method for domesticating and cultivating the Chinese caterpillar fungus, in particular to a strain of the Chinese caterpillar fungus and a method for cultivating the Chinese caterpillar fungus. Background technique [0002] Nunchaku Cordyceps ( Cordyceps ninchukispora (C.H. Su & H.H. Wang) G.H. Sung, J.M. Sung, Hywel-Jones & Spatafora) Belongs to the Kingdom Fungi, Ascomycota, Ascomycotina, Copromycetes, Hypocreatia, Hypocreales, Cordyceps Branch, Cordyceps. Fungi of the genus Cordyceps are mostly parasitic on insect larvae or adults, arthropods such as spiders, and some species of the genus Macrocystis, and produce sub-seats on the host. The Nunchaku Cordyceps that this patent relates to parasitizes on the fungus of the genus Macroma. [0003] Cordyceps sinensis is an important medicinal fungus. The research and utilization of Cordyceps sinensis in my country has a long history. It cannot be artificially cultivated, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 胡殿明张俊波
Owner JIANGXI AGRICULTURAL UNIVERSITY
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