Medicine for treating AD (alzheimer's disease) and preparation method thereof
A technology for Alzheimer's disease and drugs, applied in the field of new drug delivery systems of traditional Chinese medicine, can solve problems such as large toxic and side effects and poor treatment effect, and achieve great application potential, improve treatment effect, and enhance the effect of curative effect.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] TC@PLGA / NPs nanoparticles were prepared by the ultrasonic-assisted emulsifier evaporation method in the one-step method. Take 5 mg of TanIIA and CTan and 150 mg of PLGA and dissolve them in 10 ml of dichloromethane to obtain an oil phase solution. Take 1 ml of the oil phase and dilute with 2- Slowly drop 3 drops / s into 15ml of 1.5% PVA aqueous phase solution, sonicate with ice bath probe for 8min (over 3s, stop for 5s). Stir for 2 hours to completely evaporate the organic solvent, centrifuge at 13,000 rpm for 30 minutes, collect drug-loaded nanoparticles, add PBS to wash, resuspend and centrifuge, repeat the operation twice, and freeze-dry for 5 hours under vacuum to obtain TC@PLGA / NPs. Store at ℃.
[0030] Take 40mg of borneol raw material, put it in a 10ml volumetric flask, and dissolve it with absolute ethanol to obtain a 4mg / ml borneol ethanol solution. At the same time, take 10mg of TC@PLGA / NPs, put it in a 10ml volumetric flask, and dissolve it with distilled wate...
Embodiment 2
[0032] TC@PLGA / NPs nanoparticles were prepared by the ultrasonic-assisted emulsifier evaporation method in the one-step method. Take 10 mg of TanIIA and CTan and 200 mg of PLGA and dissolve them in 10 ml of dichloromethane to obtain an oil phase solution. Take 1 ml of the oil phase and add 2- Slowly drop 3 drops / s into 10ml 2% PVA aqueous solution, sonicate with ice bath probe for 6min (over 3s, stop for 5s). 2h, let the organic solvent evaporate completely, centrifuge at 13000rpm for 30min, collect the drug-loaded nanoparticles, add PBS to wash, resuspend and centrifuge, repeat the operation twice, freeze-dry under vacuum for 5h, get TC@PLGA / NPs, store at 4°C .
[0033] Take 40mg of borneol raw material, put it in a 10ml volumetric flask, and dissolve it with absolute ethanol to obtain a 4mg / ml borneol ethanol solution. At the same time, take 10mg of TC@PLGA / NPs, put it in a 10ml volumetric flask, and dissolve it with distilled water to get For the drug-loaded nanosuspension...
Embodiment 3
[0035] TC@PLGA / NPs nanoparticles were prepared by the ultrasonic-assisted emulsifier evaporation method in the one-step method. Take 10 mg of TanIIA and CTan and 200 mg of PLGA and dissolve them in 10 ml of dichloromethane to obtain an oil phase solution. Take 1 ml of the oil phase and add 2- Slowly drop 3 drops / s into 10ml of 1% PVA aqueous phase solution, sonicate with ice bath probe for 5min (over 3s, stop for 5s). The solvent was completely evaporated, centrifuged at 13000rpm for 30min, collected drug-loaded nanoparticles, added PBS to wash, resuspended and centrifuged, repeated twice, and freeze-dried for 5h under vacuum to obtain TC@PLGA / NPs, which were stored at 4°C.
[0036] Take 40mg of borneol raw material, put it in a 10ml volumetric flask, and dissolve it with absolute ethanol to obtain a 4mg / ml borneol ethanol solution. At the same time, take 10mg of TC@PLGA / NPs, put it in a 10ml volumetric flask, and dissolve it with distilled water to get For the drug-loaded nan...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More