A kind of medicine for treating Alzheimer's disease and preparation method thereof
A technology for Alzheimer's disease and drugs, which is applied in the field of new drug delivery system of traditional Chinese medicine, can solve the problems of high toxicity and side effects, poor treatment effect, etc., achieve huge application potential, improve treatment effect, and enhance curative effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0029] The TC@PLGA / NPs nanoparticles were prepared by the ultrasonic-assisted emulsifier evaporation method in the one-step method. 5 mg of each of TanIIA and CTan and 150 mg of PLGA were dissolved in 10 ml of dichloromethane to obtain an oil phase solution, and 1 ml of the oil phase was taken as 2- Slowly drop 3 drops / s into 15ml 1.5% PVA aqueous solution, ultrasonicate the probe in ice bath for 8min (over 3s, stop for 5s), put the nanosuspension on a magnetic stirrer at room temperature at a speed of 500rmp after ultrasonication Under stirring for 2 h, the organic solvent was completely volatilized, centrifuged at 13000 rpm for 30 min, the drug-loaded nanoparticles were collected, washed with PBS, resuspended and centrifuged, repeated twice, and freeze-dried under vacuum for 5 h to obtain TC@PLGA / NPs, which were placed in 4 Store at ℃.
[0030] Take 40mg borneol crude drug, put it in a 10ml volumetric flask, dissolve it with absolute ethanol to obtain a 4mg / ml borneol ethano...
Embodiment 2
[0032] The TC@PLGA / NPs nanoparticles were prepared by the ultrasonic-assisted emulsifier evaporation method in the one-step method. 10 mg of each of TanIIA and CTan, and 200 mg of PLGA were dissolved in 10 ml of dichloromethane to obtain an oil phase solution, and 1 ml of the oil phase was taken as 2- Slowly drop 3 drops / s into 10ml 2% PVA aqueous solution, ultrasonicate the probe in ice bath for 6min (over 3s, stop for 5s), put the nanosuspension on a magnetic stirrer and stir at room temperature at a speed of 400rmp after ultrasonication 2h, the organic solvent was completely volatilized, centrifuged at 13,000rpm for 30min, the drug-loaded nanoparticles were collected, washed with PBS, resuspended and centrifuged, repeated twice, freeze-dried under vacuum for 5h to obtain TC@PLGA / NPs, which were stored at 4°C .
[0033] Take 40mg borneol crude drug, put it in a 10ml volumetric flask, dissolve it with absolute ethanol to obtain a 4mg / ml borneol ethanol solution, take 10mg of ...
Embodiment 3
[0035] The TC@PLGA / NPs nanoparticles were prepared by the ultrasonic-assisted emulsifier evaporation method in the one-step method. 10 mg of each of TanIIA and CTan, and 200 mg of PLGA were dissolved in 10 ml of dichloromethane to obtain an oil phase solution, and 1 ml of the oil phase was taken as 2- Slowly drop 3 drops / s into 10ml of 1% PVA aqueous solution, ultrasonicate the probe in ice bath for 5min (over 3s, stop for 5s), after ultrasonication, place the nanosuspension on a magnetic stirrer and stir at room temperature for 2h to make organic After the solvent was completely evaporated, the drug-loaded nanoparticles were collected by centrifugation at 13,000 rpm for 30 min, washed with PBS, resuspended, and centrifuged.
[0036] Take 40mg borneol crude drug, put it in a 10ml volumetric flask, dissolve it with absolute ethanol to obtain a 4mg / ml borneol ethanol solution, take 10mg of TC@PLGA / NPs at the same time, put it in a 10ml volumetric flask, dissolve it with distilled...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More