Anti-inflammatory composition as well as preparation method and application thereof

An anti-inflammatory composition, the technology of magnolol, is applied in the direction of anti-inflammatory agents, drug combinations, active ingredients of hydroxyl compounds, etc., and can solve the problem of weak individual effects

Inactive Publication Date: 2018-09-04
上海璞萃生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] To sum up, both asiaticoside and magnolanol have certain anti-inflammatory effects, but their individual effects are weak

Method used

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  • Anti-inflammatory composition as well as preparation method and application thereof
  • Anti-inflammatory composition as well as preparation method and application thereof
  • Anti-inflammatory composition as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] An anti-inflammatory composition, comprising the following components: magnolan alcohol and asiaticoside; the concentration of the asiaticoside is 10 μM, and the concentration of magnolan alcohol is 10 μM, 5 μM or 2.5 μM. The concentration ratio of magnolanol and asiaticoside is 1:1.

[0021] A preparation method of an anti-inflammatory composition. Both magnolanol and asiaticoside are prepared into a mother solution with a concentration of 20mM with a solvent, then the mother solution of asiaticoside is diluted to 10 μM by using DMEM medium, and the magnolan alcohol is diluted by DMEM medium. Diluted to 10 μM, 5 μM or 2.5 μM; finally, mix the diluted mother liquor of magnolanol and mother liquor of asiaticoside evenly to obtain the product. The solvent is DMSO.

[0022] An application of an anti-inflammatory composition in the technical field of anti-inflammatory cosmetics, said anti-inflammatory cosmetics comprising the following raw materials in percentage by weight...

experiment example

[0024] RAW264.7 mouse macrophages induced by lipopolysaccharide (LPS) are a common model for studying inflammatory responses. Under the stimulation of lipopolysaccharide LPS, macrophages can promote the secretion of inflammatory factors such as nitric oxide (NO), interleukin-6 (interleukin 6) and tumor necrosis factor α (tumor necrosis factor α, TNF-α). In the present invention, LPS induces RAW264.7 cells to release NO and IL-6 as targets, and investigates the anti-inflammatory effect of the compounded anti-inflammatory composition of asiaticoside and magnolanol.

[0025] 1. Materials and reagents:

[0026] RAW264.7 cell line was purchased from Shanghai Institute of Cells, Chinese Academy of Sciences; asiaticoside, magnolanol and DMSO were purchased from Sigma Company; DMEM high-glucose medium, fetal bovine serum FBS, trypsin, etc. were purchased from Gibco Company; CCK-8 was purchased from Shanghai Qianchen Biotechnology Co., Ltd.; IL-6, and other cytokine ELISA kits were pu...

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Abstract

The invention discloses an anti-inflammatory composition as well as a preparation method and application thereof. The anti-inflammatory composition is prepared from the following components: magnololand asiaticoside, wherein the concentration of asiaticoside is 10 uM or 2.5 uM. The preparation method of the anti-inflammatory composition comprises the steps that magnolol and asiaticoside are prepared to be mother liquid with the concentration being 20 mM by using solvents, then a DMEM (dulbecco modified eagle medium) culture medium is adopted to dilute the asiaticoside mother liquid to be 10 uM, the DMEM culture medium is adopted to dilute the magnolol to be 10 uM, 5 uM or 2.5 uM; and finally the diluted magnolol mother liquid and asiaticoside mother liquid are mixed to be uniform, and theanti-inflammatory composition is obtained. The invention discovers that asiaticoside can effectively enhance the anti-inflammatory activity of low-concentration magnolol (10, 5 and 2.5 uM), magnololand asiaticoside have the synergistic effect, and the effect can be better when magnolol and asiaticoside are matched at the ratio of 1 to 1.

Description

technical field [0001] The invention relates to an anti-inflammatory product, in particular to an anti-inflammatory composition and its preparation method and application. Background technique [0002] Centella asiatica (centella asiatica) belongs to Umbelliferae, originates in India, and is now widely distributed in tropical and subtropical regions of the world. In my country, it is mainly distributed in the provinces south of the Yangtze River. Its extracts have been traditionally used topically for blemishes and inflammation. Madecassoside is the main component of Centella asiatica extract. Madecassoside is a triterpenoid saponin compound and one of the main active ingredients of the traditional Chinese medicine Centella asiatica. Traditional Chinese medicine believes that Centella asiatica has the effects of clearing heat and dampness, detoxifying and reducing swelling, and the extract of Centella asiatica containing asiaticoside has anti-inflammatory effects, and it ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61K31/05A61P29/00A61K8/60A61K8/34A61Q19/00
CPCA61K8/347A61K8/60A61K31/05A61K31/704A61K2800/10A61K2800/592A61P29/00A61Q19/00A61K2300/00
Inventor 杨海丽
Owner 上海璞萃生物科技有限公司
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