Preparation process of elastic protein

A kind of technology of elastin and preparation technology, which is applied in the field of elastin preparation technology, can solve the problems such as imperfect elastin preparation technology, and achieve the effect of high activity and pure ingredients

Inactive Publication Date: 2018-09-11
西安德氏禾本生物科技有限公司
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Problems solved by technology

[0004] At present, the market demand for elastin is increasing day by day, but the preparation process o

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Example Embodiment

[0023] The preferred embodiments of the present invention are further described below in conjunction with specific embodiments:

[0024] A preparation process of elastin includes the following steps:

[0025] In the first step, the ligaments are washed and minced and mixed evenly with phosphate buffer to obtain a ligament solution. Elastase is added to the ligament solution; the pH of the phosphate buffer is 7.8±0.5. The amount of elastase added is 1 g of elastase per 100 g of ligament solution.

[0026] The second step is to add 8.5g of NaCl per 1L of the ligament solution after adding elastase, and then perform ultrasonic treatment, and incubate at 25℃ with shaking for 2h; the ultrasonic intensity of ultrasonic treatment is 0.4W / cm 2 , The frequency is 50KHz, the power is 140W, and the ultrasonic treatment time is 20min. The rotation speed of shaking culture is 100~120r / min.

[0027] The third step, the ligament solution after the second step is filtered through multi-layer gauze a...

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Abstract

The invention discloses a preparation process of an elastic protein, which includes the steps of: 1) washing and mincing ligament, and mixing the ligament with a phosphoric acid buffer solution to obtain a ligament solution, and adding elastinase to the ligament solution; 2) adding NaCl to the ligament solution and performing ultrasonic treatment and vibration culture; 3) filtering the solution through a plurality layers of gauze and abandoning filter residue, discoloring the filtrate through active carbon, and treating the filtrate through a semi-permeable membrane; 4) collecting the elasticprotein passing through the semi-permeable membrane and dissolving the elastic protein in pure water, performing centrifugal separation and collecting a supernatant; 5) treating the supernatant through a semi-permeable membrane in an ultra-filtering apparatus. The elastic protein is pure, is high in activity and is very suitable for various fields, such as foods, medicines, cosmetics and the like.

Description

technical field [0001] The invention relates to the field of material preparation, in particular to a preparation process of elastin. Background technique [0002] Elastin is a hard protein present in ligaments and vessel walls, mainly produced by fibroblasts, aortic smooth muscle cells, and chondrocytes. It can combine with microfibrils to form elastic fibers in vivo, and its main function is passive stretching , to endow the tissues and organs with stretchability and reversible deformation ability. The content of elastin in tissues varies with the importance of elastic function to tissues. The content of elastin is the highest in ligaments, accounting for about 80%, followed by 30% to 50% in aorta, 10% to 25% in lungs, and 10% to 25% in skin. 2% to 3%. Elastin is a hard protein that is extremely difficult to dissolve. It is insoluble in dilute acid, alkali, alcohol and salt solutions, and only swells in some polar solvents; elastin has elastic properties under hydration ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34
CPCC07K14/78C12P21/06
Inventor 安航安德荣
Owner 西安德氏禾本生物科技有限公司
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