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A bacterial agent comprising Brevibacterium nirzia

A technology of Brevibacterium nissleri and bacterial agents, applied in the field of bacterial agents containing Brevibacterium nierlii NY-1, which can solve the problems of low treatment efficiency of volatile organic chlorine, uneven loading, and packing collapse, etc. Lightweight, shortened start-up period, and small pressure loss

Inactive Publication Date: 2019-08-13
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In order to solve the problems of low volatile organic chlorine treatment efficiency, uneven loading of microorganisms on the surface of the mixed carrier, slow start-up, and collapse of fillers after long-term use in the prior art, the present invention provides a strain of Brachybacterium nesterenkovii NY- 1, and apply the strain to remove waste gas containing organic chlorine

Method used

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  • A bacterial agent comprising Brevibacterium nirzia
  • A bacterial agent comprising Brevibacterium nirzia

Examples

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Effect test

Embodiment 1

[0042] Example 1: A bacterial agent for purifying exhaust gas containing organic chlorine and its application

[0043] The present invention also provides an inoculum prepared from Brevibacterium niersii NY-1, which can be used to purify exhaust gas containing organic chlorine and solve the problem of volatile organic chlorine pollution generated in processes such as pesticide wastewater treatment. The preparation method of the agent is as follows:

[0044] (1) Preparation method of fermentation broth

[0045] Brevibacterium niersii NY-1 was cultured on a slope and then connected to the liquid fermentation medium, and then scaled up and cultivated step by step according to the 10% inoculum. The fermentation and culture conditions were: 36°C, ventilation 10-100L / min, dissolved oxygen> Under the condition of 2.0mg / L, tank pressure 0.03-0.10Mpa, culture time is 48h;

[0046] The composition of the culture medium for slant culture is: glucose 0.5g, KH 2 PO 4 2.0g, NaHCO 3 2.0g, KNO 3 2....

Embodiment 2

[0053] Example 2: A bacterial agent for purifying exhaust gas containing organic chlorine and its application

[0054] The present invention also provides an inoculum prepared from Brevibacterium niersii NY-1, which can be used to purify exhaust gas containing organic chlorine and solve the problem of volatile organic chlorine pollution generated in processes such as pesticide wastewater treatment. The preparation method of the agent is as follows:

[0055] (1) Preparation method of fermentation broth

[0056] Brevibacterium niersii NY-1 was cultured on a slope, and 25% of the inoculum was inserted into the liquid fermentation medium for gradual amplification. The fermentation and culture conditions were: 36°C, ventilation 10-100L / min, dissolved oxygen > Under the condition of 2.0mg / L, tank pressure 0.03-0.10Mpa, culture time 72h;

[0057] The composition of the culture medium for slant culture is: glucose 0.5g, KH 2 PO 4 2.0g, NaHCO 3 2.0g, KNO 3 2.0g, NH 4 Cl 0.5g, MgCl 2 ·6H 2 O...

Embodiment 3

[0064] Example 3: A bacterial agent for purifying exhaust gas containing organic chlorine and its application

[0065] The present invention also provides an inoculum prepared from Brevibacterium niersii NY-1, which can be used to purify exhaust gas containing organic chlorine and solve the problem of volatile organic chlorine pollution generated in processes such as pesticide wastewater treatment. The preparation method of the agent is as follows:

[0066] (1) Preparation method of fermentation broth

[0067] Brevibacterium niersii NY-1 was cultured on an inclined plane and then connected to the liquid fermentation medium. The culture was amplified step by step according to the 15% inoculum. The fermentation and culture conditions were: 36℃, ventilation 10-100L / min, dissolved oxygen> Under the condition of 2.0mg / L, tank pressure 0.03-0.10Mpa, culture time is 24h;

[0068] The composition of the culture medium for slant culture is: glucose 0.5g, KH 2 PO 4 2.0g, NaHCO 3 2.0g, KNO 3 2...

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Abstract

The invention belongs to the field of environmental treatment and particularly relates to a brachybacterium nesterenkovii-containing microbial agent. Specifically, applied brachybacterium nesterenkovii is brachybacterium nesterenkovii NY-1 with a preservation number of CGMCC No. 13747. The brachybacterium nesterenkovii-containing microbial agent is high in organochlorine degrading efficiency of upto 90-96%, short in adaptive period of dominant bacteria, and capable of rapidly starting degrading reaction within one week, and when applied to a biological reactor for purifying organochlorine-containing waste gas, can shorten the starting period.

Description

[0001] Technical field: [0002] The invention belongs to the field of environmental treatment, and specifically relates to a bacterial agent containing Brachybacterium nesterenkovii NY-1. [0003] Background technique: [0004] Organochlorine pesticides are organic compounds that contain organochlorine elements in the components used to prevent and control plant diseases and insect pests, and are recognized as a very important type of persistent organic pollutants. In the process of organochlorine pesticide production and pesticide wastewater treatment, volatile pesticide products or intermediates volatilize from the liquid phase and escape into the air to form air pollution. Volatile pesticides and intermediates include: trichlorfon, dichlorvos, phosphorus trichloride, DDT, chloral urea, sodium trichloroacetate, BHC, aldrin, chlordane, endrin, seven Chlorine, toxaphene, hexachlorobenzene, mirex, chloral, etc., most of these substances have a pungent odor, some substances such as c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/85B01D53/70C12R1/01
CPCB01D53/685B01D53/70B01D53/85B01D2251/95B01D2257/2064C12N1/20Y02A50/20
Inventor 刘俊新杨唐李琳李文凯
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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