TK gene mutation testing method and mutagenesis strength determining method suitable for evaluating genotoxicity of cigarette smoke condensate

Abstract

The invention relates to a TK gene mutation testing method and a mutagenesis strength determining method suitable for evaluating genotoxicity of cigarette smoke condensate and belongs to the technicalfield of cigarette harm evaluation. The cigarette smoke condensate is adopted to directly treat tested cells, so that mutagenesis action of the cigarette smoke condensate can be judged qualitativelyand quantitatively, toxic effect of cigarettes can be judged simply and quickly, and the problems that genotoxicity end points are few, smoke is prone to aging and toxicity-presenting dosage range isnarrow in the process of cigarette smoke genotoxicity detection are solved; the TK gene mutation testing method and the mutagenesis strength determining method are applicable to cigarettes on the market, can realize quantitative judgment of genotoxicity of cigarette smoke and horizontal comparison of TK gene mutagenesis action of different cigarettes and are wide in application range.

Description

technical field [0001] The invention relates to a TK gene mutation test method and a mutagenic strength determination method suitable for evaluating the genotoxicity of cigarette smoke condensate, and belongs to the technical field of cigarette hazard evaluation. Background technique [0002] Cigarette smoke contains nearly 5,315 compounds, 158 of which have been identified to be toxic to humans or experimental animals. How to evaluate the hazards of cigarette smoke has always been the direction of research by relevant researchers at home and abroad. In 2004, the Tobacco Smoke In vitro Toxicity Testing Working Group of the International Tobacco Scientific Research Cooperation Center (Cooperation center for scientific research relation to tobacco, CORESTA) recommended three in vitro toxicity tests to evaluate the harm of cigarette smoke, namely cytotoxicity test, Salmonella typhimurium reverse mutation test (Ames test) and micronucleus test in vitro. [0003] With the in-dep...

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Problems solved by technology

[0004] Although there are domestic MLA methods related to food, food additives, and food contact materials, cigarette smoke is quite different from food, food additives, and food contact materials, and cannot be used directly. For example, smoke is easy to age, The narrow range of doses exhibiting toxicity makes it particularly urgent to establish an MLA applicable to cigarette smoke

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