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Method of utilizing fluorescence quenching to detect forchlorfenuron in Kiwi berries

A fluorescence quenching and kiwifruit technology, applied in the field of food detection and chlorfenuron, can solve the problems of unsuitable for routine detection of chlorfenuron in kiwifruit, complex sample pretreatment process, and high technical requirements, so as to achieve smooth background interference, The effect of eliminating baseline drift and overcoming redundancy problems

Active Publication Date: 2018-09-18
SHAANXI NORMAL UNIV
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Problems solved by technology

[0003] Although the existing detection methods are technically mature and have high precision, they have high technical requirements, complicated sample pretreatment process, time-consuming, high detection cost, and need to use a large number of chemical reagents in the detection process, which is easy to cause environmental pollution and is difficult to detect. On-site rapid and green detection, not suitable for routine detection of forchlorfenuron residues in kiwifruit

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  • Method of utilizing fluorescence quenching to detect forchlorfenuron in Kiwi berries
  • Method of utilizing fluorescence quenching to detect forchlorfenuron in Kiwi berries
  • Method of utilizing fluorescence quenching to detect forchlorfenuron in Kiwi berries

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Embodiment 1

[0034] The method that present embodiment detects forchlorfenuron in kiwifruit is realized by the following steps:

[0035] (1) Weigh 8.00g of the kiwifruit sample to be tested in a 50ml centrifuge tube, make a pulp, add 16ml of ethyl acetate as the extractant, ultrasonically shake for 20min, add 1.2g of anhydrous MgSO 4 and 0.8g NaCl, vortex for 5min, let it stand for 30min, put it into a centrifuge and set it at 5000r / min and centrifuge for 10min to obtain 500ml supernatant, which is used as the sample extract to be tested.

[0036] (2) Add 0.5ml of the extract of the sample to be tested obtained in step (1) to a concentration of 1.5×10 -5 In the bovine serum albumin solution of mol / L, collect the quenched fluorescence emission spectrum of the sample to be tested; the fluorescence spectrum adopts the RF-6000 fluorescence spectrophotometer of Japan Shimadzu Company, and the collection parameters: excitation and emission monochromator slit width Both are 5nm, the scanning spe...

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Abstract

The invention relates to a method of utilizing fluorescence quenching to detect forchlorfenuron in Kiwi berries. According to the method, forchlorfenuron in Kiwi berries is detected by fluorescence quenching of bovine serum albumin (BSA), a two-dimensional related spectrum, and a neural network. The data of the main diagonal of a synchronous two-dimensional fluorescence spectrum is extracted. Thedifference change information that changes along with the external interference can be represented; trace forchlorfenuron can be detected; the treatment is simple, the analysis efficiency and accuracyare high, the detection is more accurate and rapid; the method can be widely used in the forchlorfenuron detection of Kiwi berries; the effect that forchlorfenuron quenches fluorescence of BSA, a two-dimensional related spectrum, and a BP neutral network are combined to detect the forchlorfenuron content of Kiwi berries; and the redundancy caused by directly using synchronous spectrum matrix datato establish a model is avoided.

Description

technical field [0001] The invention relates to a method for detecting forchlorfenuron in kiwi fruit by utilizing the quenching effect of forchlorfenuron on the fluorescence of bovine serum albumin (BSA) combined with two-dimensional correlation spectrum and BP neural network, and belongs to the field of food detection methods. Background technique [0002] Kiwi fruit has unique flavor, delicious taste, rich in vitamin C, dietary fiber and various mineral nutrients, and has many health benefits. It is one of the important fruits favored by the world today. With people's in-depth understanding of kiwifruit and the rise of global commercial development, China's kiwifruit industry has developed rapidly, and its cultivation area and output both rank first in the world. Because forchlorfenuron has excellent growth-promoting effects, especially in promoting fruit expansion, it is applied to kiwifruit. However, the abuse of forchlorfenuron during the growth of kiwifruit seriously ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 赵武奇李丽娜卢丹曾祥媛吴妮
Owner SHAANXI NORMAL UNIV