Bearing device for cell vitrification and automatic operation system for bearing device

Abstract

The invention provides a bearing device for cell vitrification. The bearing device comprises a droplet bearer; the droplet bearer is of a rectangular shallow tray structure, and comprises a cell bearing region in which a frozen carrier is put at the middle, and a hydrophobic region surrounding the cell bearing region; the hydrophobic region is of an annular structure laid with a super-hydrophobicmaterial; the middle parts of two sides of the droplet bearer are provided with dark-colour marked lines respectively; and the two dark-colour marked line are located on the central axis of the droplet bearer. The bearing device enables living cells to rapidly and effectively realize vitrification.

Description

technical field [0001] The invention belongs to the technical field of cryopreservation and recovery of human tissue, and in particular relates to a bearing device for cell vitrification and freezing and an automatic operating system thereof. Background technique [0002] The basic principle of vitrification freezing method is to solidify a high concentration of cryoprotectant in an ultra-low temperature environment to form an irregular vitrified solid, which preserves the normal distribution of molecules and ions in the liquid state, so that it can act when vitrification occurs in cells. to protection. Today's freezing technology can be used to cryopreserve sperm, eggs and embryos at different stages, especially vitrification technology, which has not only been widely recognized by the medical community, but has gradually replaced the traditional slow freezing method and has become the most advanced freezing technology. best choice. [0003] Most of the existing technolog...

AI Technical Summary

Problems solved by technology

This manual operation method requires the time to be as short as possible and the coating surface to be as thin as possible to cool down quickly; this operation often requires the operator to have skilled operating skills, and the operator is required to set the droplet into a gradient concentration solution, so that the cells in the gradient This process requires rapid operation. Improper operation may lead to abnormal freezing of living cells due to excessive osmotic pressure or inability to standardize the operation process, and the cells are easily dead or lost.

Images