NESS1-A gene having high deodorization ability on lactating cow excrement of bombina orientalis intestinal bacillus expression proteins

Abstract

The invention discloses an NESS1-A gene having the high deodorization ability on lactating cow excrement of bombina orientalis intestinal bacillus expression proteins, and belongs to the technical field of animal biology. The NESS1-A gene is characterized in that the nucleotide sequence of the NESS1-A gene having the high deodorization ability on the lactating cow excrement of the bombina orientalis intestinal bacillus expression proteins is shown in Seq ID No:1, lactating cow fresh excrement mixed with a to-be-measured sample solution is loaded in three disinfected 24-hole cell culture platesin a split mode and cultivated in an illumination incubator at the temperature of 37 DEG C, mixtures in the 24-hole cell culture plates with the corresponding serial numbers are taken out every otherhour, then the content of ammonia nitrogen in the mixtures is measured through an ammonia nitrogen meter, the deodorization ability of the expression proteins on the lactating cow excrement is calculated, and the result shows that after seven hours, the content of the ammonia nitrogen in the mixtures is zero, therefore it is shown that the expression proteins of the bombina orientalis intestinalbacillus NESS1-A gene has the high deodorization ability on the lactating cow excrement.

Description

technical field [0001] The invention relates to a NESS1-A gene whose protein expressed by Bacillus orientalis intestinalis has a strong deodorizing ability to the feces of lactating dairy cows, and belongs to the field of animal biotechnology. Background technique [0002] With the rapid development of the cattle industry, feces pollution has become a major problem. A cow produces more than 7 tons of feces every year. Due to the general lack of attention to the treatment of cow dung in various places, there are basically no cow dung treatment facilities in some places where cattle are more concentrated, which leads to piles of cow dung everywhere, especially in summer, and the stench is so bad that it is harmful to the normal life of the surrounding residents. At the same time, cow dung is the source of a variety of bacterial pathogens, which has a serious impact on the breeding population. In addition, the raw cow dung on the ground generates heat, consumes soil oxygen, ca...

AI Technical Summary

Problems solved by technology

Due to the general lack of attention to the treatment of cow dung in various places, there are basically no cow dung treatment facilities in some places where cattle are more concentrated, which leads to piles of cow dung everywhere, especially in summer, and the stench is so bad that it is harmful to the normal life of the surrounding residents. At the same time, cow dung is the source of the reproduction of a variety of bacterial pathogens, which has a serious impact on the breeding population

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