SNP locus of crassostrea gigas AHR gene

A long oyster, site technology, applied in the field of genetic breeding, can solve the problems of slow growth, long oyster germplasm degradation, increased mortality, etc., to achieve fast growth and reduce blindness

Active Publication Date: 2018-09-28
ZHEJIANG WANLI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, while my country's long oyster aquaculture industry is developing steadily, it is also beginning to face many serious problems: with the continuous improvement of aquaculture intensification and the frequent occurrence of marine environmental pollution and red tides, the aquaculture environment of long oysters is deteriorating day by day; Breeding leads to inbreeding, and the germplasm degradation of cultured oysters is becoming more and more serious. Problems such as slow growth, low meat yield, and increased mortality have occurred, which seriously affect the sustainable development of the oyster industry; with the improvement of consumption levels and the market With the intensification of competition, the domestic and foreign consumer markets have continuously improved the quality requirements of long oysters

Method used

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  • SNP locus of crassostrea gigas AHR gene
  • SNP locus of crassostrea gigas AHR gene
  • SNP locus of crassostrea gigas AHR gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Acquisition of markers associated with rapid growth of the long oyster

[0018] 1. On the basis of the complete sequencing of the long oyster genome, the genes related to growth—bHLH family were obtained through GWAS association analysis technology. The bHLH family of oyster oyster was classified, including 89 genes from groups A to F. On this basis, 5 candidate genes were selected for SNP screening by resequencing, and a wild population of 417 individuals was selected. The obtained 63 loci were verified by gene chip method in the wild population of 288 individuals, and finally the association analysis with growth traits was carried out with biological software to obtain the Newchr17_8788640 SNP marker. In Newchr17_8788640-marked individuals, the growth rate of AA genotype individuals was significantly higher than that of CA genotype and CC genotype individuals.

[0019] 2. Association analysis of different genotypes and growth traits of SNP loci in Oyster o...

Embodiment 2

[0026] Example 2: Application of SNP markers in screening fast-growing long oyster parents

[0027] A kit for evaluating and screening the SNP markers of fast-growing long oyster parents was made to detect the SNP site Newchr17_8788640 of the AHR gene.

[0028] The kit contains reagents for specific amplification primers and specific extension primers required to detect SNP sites, wherein the specific amplification primer sequences for site Newchr17_8788640 are SEQ ID NO: 2 and SEQ ID NO: 3. The kit also includes common reagents required by corresponding techniques such as (10×PCR (containing Mg2+) buffer, dNTP (10mM), double distilled water, enzymes, etc. In addition, there may be standard products and controls such as determined genes).

[0029] The amplification reaction system includes: 1.0 μL of cDNA, 2.5 μL of 10×PCR (containing Mg2+) buffer, 2.0 μL of dNTP with a concentration of 10 mM, 1.0 μL of an upstream primer with a concentration of 10 μM, 1.0 μL of a downstream p...

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Abstract

The invention provides an SNP locus related to growth of crassostrea gigas. The SNP locus is positioned in a gene AHR, and is a 269th position from a 5' end of the sequence being SEQ ID NO: 1, and thebasic group of the SNP locus is C or A; and the growth speed of an AA genotype individual is remarkably higher than the growth speeds of CA genotype and CC genotype individuals. By application of thediscovered SNP label, the blindness of selection of parents of the crassostrea gigas can be greatly reduced, and crassostrea gigas individuals with high growth speed and stable inheritance can be quickly obtained for breeding or culture.

Description

technical field [0001] The invention belongs to the technical field of genetic breeding, and in particular relates to a SNP locus related to the rapid growth of oysters. Background technique [0002] The long oyster (Crassostrea gigas), also known as the Pacific oyster, is the most widely cultivated and produced marine economic shellfish in the world, and it is also an important traditional cultured species in my country. At present, while my country's long oyster aquaculture industry is developing steadily, it is also beginning to face many serious problems: with the continuous improvement of aquaculture intensification and the frequent occurrence of marine environmental pollution and red tides, the aquaculture environment of long oysters is deteriorating day by day; Breeding leads to inbreeding, and the germplasm degradation of cultured oysters is becoming more and more serious. Problems such as slow growth, low meat yield, and increased mortality have occurred, which serio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 包永波陈娜林志华王素芳
Owner ZHEJIANG WANLI UNIV
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