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Method for detecting chelation rate of small peptide-trace element chelate

A technology for trace elements and detection methods, which is used in measurement devices, measurement of color/spectral properties, and material analysis by optical means.

Active Publication Date: 2018-10-02
HUNAN DEBANG BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The present invention provides a small peptide trace element chelate in view of the existing small peptide trace element chelate chelation rate detection method, which still partially dissolves when the organic solvent is extracted, resulting in inaccurate measurement results. The detection method of compound chelation rate, the accuracy of the measurement results is higher by this method, and the detection process is simple to operate and easy to popularize and apply

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  • Method for detecting chelation rate of small peptide-trace element chelate
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  • Method for detecting chelation rate of small peptide-trace element chelate

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Embodiment 1

[0035] Chelation rate determination method of small peptide copper chelate:

[0036] Measure the copper content in the small peptide copper chelate sample by atomic absorption spectrophotometer; take another 800mg of the small peptide copper chelate to be tested and add it to 3mL of methanol at a temperature of 5°C, and shake it with a constant temperature oscillator for 10min and then 12000r / min Centrifuge for 15 minutes, take the supernatant, and measure the free copper content in the supernatant. The copper content is determined using an atomic absorption spectrophotometer, and the solute-solvent ratio is 800:3 mg / mL.

[0037] According to the formula, the chelation rate of the small peptide copper chelate is 94.97%.

Embodiment 2

[0039] Chelation rate determination method of small peptide zinc chelate:

[0040] Determine the zinc content in the small peptide zinc chelate sample by atomic absorption spectrophotometer; take another 600mg of the small peptide zinc chelate to be tested and add it to 3mL of methanol at a temperature of 20°C, shake with a constant temperature oscillator for 30min and then 12000r / min After centrifugation for 15 min, the supernatant was taken to measure the free zinc content in the supernatant. The zinc content was determined using an atomic absorption spectrophotometer, and the solute-solvent ratio was 600:3 mg / mL.

[0041] According to the formula, the chelation rate of the small peptide zinc chelate is 99.65%.

Embodiment 3

[0043] Chelation rate determination method of small peptide copper chelate:

[0044] Measure the copper content in the small peptide copper chelate sample by atomic absorption spectrophotometer; take another 600mg of the small peptide copper chelate to be tested and add it to 3mL of methanol at a temperature of 2°C, shake with a constant temperature oscillator for 30min and then 12000r / min Centrifuge for 15 minutes, take the supernatant, and measure the free copper content in the supernatant. The copper content is measured using an atomic absorption spectrophotometer, and the solute-solvent ratio is 600:3 mg / mL.

[0045] According to the formula calculation, the chelation rate of the small peptide copper chelate is 90%.

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Abstract

The invention relates to a method for detecting the chelation rate of small peptide-trace element chelate. The method has the advantages that a methanol extraction method is used to separate free-state metal ions and chelating-state metal ions, optimal solute-solvent ratio, extraction temperature and extraction time are provided, and the dissolving quantity of small peptide-copper chelate in methanol is lowered by the combination of the three optimized parameters so as to increase detection accuracy; the method does not need early-stage product processing and is simple to operate, fast, accurate in detection result, applicable to the measuring of the chelation rate of the small peptide-copper chelate and small peptide-zinc chelate and capable of satisfying fast and accurate detection requirements in production.

Description

technical field [0001] The invention relates to the field of element detection, in particular to a method for detecting the chelation rate of small peptide trace element chelates. Background technique [0002] Small peptide trace element chelate is a chelate with a ring structure formed by the combination of metal ions and small peptide peptide bond amino nitrogen atoms and carboxyl oxygen atoms through coordination bonds. It is another new type of amino acid trace element chelate. Organic trace element additive. Compared with other inorganic trace elements such as hydrochloride and sulfate or organic acid trace elements, on the one hand, it has the characteristics of high absorption rate, high biological utilization rate, and stable structure in the gastrointestinal tract; The addition of medium and low levels has the effects of promoting growth, improving the intestinal environment, and improving the body's antioxidant and immune functions. Therefore, adding low-level sm...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/3103
Inventor 徐维娜徐建雄李少华张京
Owner HUNAN DEBANG BIOLOGICAL TECH CO LTD
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