Method for constructing animal model of the rabbit knee cartilage subchondral bone injury and method for evaluating osteogenic material
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A subchondral bone, animal model technology, applied in the field of biomedicine, can solve problems such as joint instability, no treatment involved, slow disease progression, etc.
Inactive Publication Date: 2018-10-09
PEOPLES HOSPITAL PEKING UNIV
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However, the existing animal models involving knee subchondral bone injury and KOA only cla...
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[0040] (1) Animals
[0041] New Zealand rabbit (GB 14922-1994); body weight: 4.5±0.2kg; sex: male or female.
[0042] (2) Equipment and materials
[0043]Orthopedic surgical instruments, product supporting equipment, direct digital flat panel X-ray imaging system (DR), conventional surgical instruments, anesthetics, emergency medicines, disinfection drugs, operating room consumables, etc.
[0044] (3) Method
[0045] 1. No feeding before operation: Animals should not be fed with water for 24 hours before operation.
[0046] 2. Preoperative blank blood
[0047] Before anesthesia, 2ml of venous blood was collected from the ear vein for determination of blood phase and serum biochemistry.
[0048] 3. Anesthesia
[0049] The animals were weighed and anesthetized by injecting 4% sodium pentobarbital (sigma, P3761-25g; CAS. Number: 57-33-0) at a dose of 1.0ml / kg into the ear vein.
[0050] 4. Skin preparation
[0051] The supine position was fixed, the hair near the knees was...
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Abstract
The invention discloses a method for constructing animal model of the rabbit knee cartilage subchondral bone injury and a method for evaluating osteogenic material. The method for constructing animalmodel of the rabbit knee cartilage subchondral bone injury comprises the following steps of: (1) carrying out general anesthesia treatment on the test New Zealand rabbits and fixing in supine position; (2) carrying out skin preparation on one side knee joint of the New Zealand rabbit; (3)carrying out the longitudinal incision by using the knee joint as the center, cutting open along the gap of themedial side of the patella and the quadriceps femoris, and turning the patella to expose the joint cavity and the articular surface of the tibial plateau,; (4) longitudinally forming a cylindrical hole in the median longitudinal direction of the joint surface of the tibial plateau by using a corneal ring drill so as to obtain an animal model of the rabbit knee cartilage subchondral bone injury. The rabbit knee cartilage subchondral bone injury animal model can effectively evaluate the osteogenic drug or the osteogenic material, thereby providing a reliable basis for screening and evaluating the osteogenic drug or the osteogenic material.
Description
technical field [0001] The invention relates to the field of biomedicine, in particular, the invention relates to a method for constructing an animal model of rabbit knee subchondral bone injury and a method for evaluating osteogenic materials. Background technique [0002] Knee Osteoarthritis (KOA) animal models are diverse, including animal sources, and production methods. The reproducibility of animal models is crucial for elucidating the mechanism of OA disease, and it is also more helpful to apply the experimental results as soon as possible. clinical. Among the commonly used OA models, in addition to different animal choices (mice, rats, guinea pigs, rabbits, dogs, goats, horses, etc.), the preparation methods also have their own characteristics. The main methods are: 1. Spontaneous formation, commonly used animals include : Rodents, dogs, horses, such as: C57 / BI6, Balb / c mice, treadmill-trained rats, guinea pigs, etc. Evaluation: Clinically relevant, but the disease ...
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