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Method of using high-concentration organic wastewater of milk factories to cultivate green alga A. dimorphus to prepare lipids and polysaccharides

An organic wastewater, high-concentration technology, applied in the field of high-value chemicals lipids and polysaccharides, to eliminate interference and simplify culture conditions

Inactive Publication Date: 2018-10-09
TIANJIN UNIV
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Problems solved by technology

Although the production of algal biomass has been extensively studied, this topic still requires the attention of researchers due to the limitations of various waste sources in algal cultivation.

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  • Method of using high-concentration organic wastewater of milk factories to cultivate green alga A. dimorphus to prepare lipids and polysaccharides
  • Method of using high-concentration organic wastewater of milk factories to cultivate green alga A. dimorphus to prepare lipids and polysaccharides
  • Method of using high-concentration organic wastewater of milk factories to cultivate green alga A. dimorphus to prepare lipids and polysaccharides

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Embodiment Construction

[0038] The present invention will be further described below through specific embodiments and accompanying drawings. The embodiments of the present invention are for better understanding of the present invention by those skilled in the art, and do not limit the present invention in any way.

[0039] The present invention utilizes the high-concentration organic waste water of milk factory to cultivate the green algae A.dimorphus to prepare the method for lipid and polysaccharide, comprises the following steps:

[0040] 1) Wastewater pretreatment: Milk wastewater (DWW) is filtered and stored in a refrigerator at 4°C without any dilution or sterilization;

[0041] Among them, phosphate in milk wastewater (DWW) is 5.96±0.04mg / L, ammonia nitrogen is 277.40±10.75mg / L, chemical oxygen demand (COD) is 2593.33±277.37mg / L, total organic carbon (TOC) is 116.23±4.3, the total Inorganic carbon (TIC) 373.20±5.47mg / L; 2) Cultivation of green algae A.dimorphus: Add 500ml of unsterilized and ...

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Abstract

The invention discloses a method of using high-concentration organic wastewater of milk factories to cultivate the green alga A. dimorphus to prepare lipids and polysaccharides. The method comprises the main steps of 1) pretreating wastewater; 2) cultivating the green alga A. dimorphus; 3) measuring lipid content in the green alga A. dimorphus; 4) measuring polysaccharide content in A. dimorphus.The method herein helps reduce the treatment cost of DWW (dairy wastewater) and produce high-value chemicals. The green alga A. dimorphus cultivated herein has about 25% of lipids and about 30% of polysaccharides, which may be further converted into biodiesel and bio-alcohol.

Description

technical field [0001] The invention relates to the field of biotechnology and environmental protection, in particular to a research method for cultivating chlorella by using high-concentration organic wastewater from a milk factory to produce high-value chemical lipids and polysaccharides. Background technique [0002] Dairy industry is one of the important industries with great economic significance in agriculture. Steady increase in demand for milk and dairy products in many countries has led to huge growth in the world dairy industry. Milk wastewater (DWW) typically contains milk solids, fat, nutrients, lactose, detergents and sanitizers (USDA-SCS, 1992) and is characterized by high biological oxygen demand (BOD) (40-48000 mg / L) and chemical Oxygen demand (COD) (80-95000mg / L) [1] . Its pH ranges from 4.7 to 11 [2] . It also contains Na, Cl, K, Ca, Mg, Fe, CO, Ni, Mn and other metals [3] , and a lot of nutrients, total nitrogen 14-830mg / L and total phosphorus 9-280m...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12P19/04C12R1/89
CPCC12P7/64C12P19/04Y02E50/10
Inventor 宋春风张尔雅凌亿锋吕希晰宋晨万芮含刘铮铮王立国
Owner TIANJIN UNIV
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