A method for ultrasonically assisted hydroenzymatic extraction of okra seed oil and protein
An okra seed, ultrasonic-assisted technology, applied in chemical instruments and methods, ultrafiltration, fat oil/fat production, etc., can solve the problems of low free oil extraction rate, insufficient comprehensive utilization of protein, and high residual oil rate in emulsions, and achieve equipment The effect of high technology maturity, promotion of comprehensive utilization, and improvement of extraction rate
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Embodiment 1
[0025] 1) The okra seeds are sequentially screened, washed, and air-dried at 50°C for 24 hours, crushed at high speed by a traditional Chinese medicine grinder, and passed through a 40-mesh sieve to obtain the okra seed powder; the okra seed powder and water are mixed at a ratio of 1:4 (w The solid-liquid ratio of / v) is mixed, the pH value is adjusted to be 7.5, and then the compound enzyme equivalent to 1% of the okra seed powder mass is added, and the compound enzyme includes cellulase and pectinase with a mass ratio of 2:1:1 and protease; then, under the conditions of temperature 50°C and ultrasonic power 200W, ultrasonic-assisted enzymolysis reaction was carried out for 60 minutes to obtain enzymolysis solution;
[0026] 2) Centrifuge the enzymatic solution obtained in step 1) at 2500r / min for 15 minutes to obtain free oil I, emulsion I, protein solution and waste residue distributed from top to bottom;
[0027] 3) after appropriate dilution of the protein solution obtain...
Embodiment 2
[0032] 1) The okra seeds are sequentially screened, washed, and air-dried at 60°C for 24 hours, crushed at a high speed by a traditional Chinese medicine grinder, and passed through a 40-mesh sieve to obtain the okra seed powder; the okra seed powder and water are mixed at a ratio of 1:5 (w The solid-liquid ratio of / v) is mixed, the pH value is adjusted to be 8.0, and then the compound enzyme equivalent to 1% of the quality of the okra seed powder is added, and the compound enzyme includes cellulase and pectinase with a mass ratio of 2:1:1 and protease; then, under the conditions of temperature 60°C and ultrasonic power 200W, ultrasonic-assisted enzymolysis reaction was carried out for 120 minutes to obtain enzymolysis solution;
[0033] 2) Centrifuge the enzymatic solution obtained in step 1) at 2500r / min for 15 minutes to obtain free oil I, emulsion I, protein solution and waste residue distributed from top to bottom;
[0034] 3) after appropriate dilution of the protein so...
Embodiment 3
[0039]1) The okra seeds are sequentially screened, washed, and air-dried at 50°C for 24 hours, crushed at high speed by a traditional Chinese medicine grinder, and passed through a 40-mesh sieve to obtain the okra seed powder; the okra seed powder and water are mixed at a ratio of 1:4 (w The solid-liquid ratio of / v) is mixed, the pH value is adjusted to be 7.5, and then the compound enzyme equivalent to 1% of the okra seed powder mass is added, and the compound enzyme includes cellulase and pectinase with a mass ratio of 2:1:1 and protease; then, under the conditions of temperature 40°C and ultrasonic power 100W, ultrasonic-assisted enzymolysis reaction was carried out for 240min to obtain enzymolysis solution;
[0040] 2) Centrifuge the enzymatic solution obtained in step 1) at 2500r / min for 15 minutes to obtain free oil I, emulsion I, protein solution and waste residue distributed from top to bottom;
[0041] 3) after appropriate dilution of the protein solution obtained in...
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