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Multiple-effect powdery composite EM bacterial agent and active activated culture and application thereof

A technology of EM bacteria and powder, which is applied in the direction of bacteria, fungi, biochemical equipment and methods, etc., can solve the problems of high price and uneven effect, and achieve the effect of convenient use, low cost and simple cultivation method

Active Publication Date: 2018-10-12
JIANGSU DAYOU BIOLOGICAL DEVAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The EM bacteria used in the breeding environment are uneven in effect, most of them are liquid, and the price is high

Method used

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  • Multiple-effect powdery composite EM bacterial agent and active activated culture and application thereof
  • Multiple-effect powdery composite EM bacterial agent and active activated culture and application thereof
  • Multiple-effect powdery composite EM bacterial agent and active activated culture and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The simple and easy expansion culture method of the multi-effect powdery composite EM bacterial agent that embodiment 1 provides, comprises the following steps:

[0025] (1) The pre-mixed solid fermentation medium is fully dissolved in water, the pH is adjusted to 6 with sodium hydroxide, the compound EM bacterial agent is added to the pre-mixed solid fermentation medium solution at a ratio of 1‰, and the container is covered and sealed with a lid;

[0026] (2) After standing for 7 days in an environment with a temperature of 30°C, the pH of the fermentation end point is 3.5, and it can be used immediately.

[0027] (3) Wherein, described initial EM bacterial agent is made up of the bacterial classification of following content:

[0028]

[0029]

[0030] The mass percent of each raw material after adding water to the premixed solid fermentation medium is:

[0031]

Embodiment 2

[0033] The simple and easy expansion culture method of the multi-effect powdery composite EM bacterial agent that embodiment 2 provides, comprises the following steps:

[0034] (1) The pre-mixed solid fermentation medium is fully dissolved in water, the pH is adjusted to 7 with sodium hydroxide, the compound EM bacterial agent is added to the pre-mixed solid fermentation medium solution at a ratio of 0.1‰, and the container is covered and sealed with a lid;

[0035] (2) After standing for 6 days in an environment with a temperature of 35°C, the pH of the fermentation end point is 4.0, and it can be used immediately.

[0036] (3) Wherein, described initial EM bacterial agent is made up of the bacterial classification of following content:

[0037]

[0038] The mass percent of each raw material after adding water to the premixed solid fermentation medium is:

[0039]

[0040]

Embodiment 3

[0042] The simple and easy expansion culture method of the multi-effect powdery compound EM bacterial agent that embodiment 3 provides, comprises the following steps:

[0043] (1) The pre-mixed solid fermentation medium is fully dissolved in water, the pH is adjusted to 8 with sodium hydroxide, the compound EM bacterial agent is added to the pre-mixed solid fermentation medium solution at a ratio of 0.01‰, and the container is covered and sealed with a lid;

[0044] (2) After standing for 5 days in an environment with a temperature of 40°C, the pH of the fermentation end point is 4.0, and it can be used immediately.

[0045] (3) wherein, the mass percent of each raw material after adding water to the premixed solid fermentation medium is:

[0046]

[0047] The mass percent of each raw material after adding water to the premixed solid fermentation medium is:

[0048]

[0049]

[0050] Control group: a blank control group.

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Abstract

The invention disclose a multiple-effect powdery composite EM bacterial agent, a premixed solid fermentation culture medium and a simple expanding culture method of the multiple-effect powdery composite EM bacterial agent. The method comprises the following steps of 1, packaging the composite EM bacterial agent and the premixed solid fermentation culture medium separately; 2, for farmlands, ponds,riverways, colony houses and other places where EM bacterial liquid is needed, using water to fully dissolve the premixed solid fermentation culture medium, using sodium hydroxide to adjust the pH to5-8, afterwards, adding the composite EM bacterial agent according to the proportion of 0.01-1%o, uniformly stirring the materials, and using a lid to cover a container for sealing; 3, placing the container in an environment within a proper temperature range of 25-40 DEG C for 5-7 days so that the product can be utilized; 4, conducting fermentation, wherein the pH in the fermentation end is 3.5-4.0. By means of the multiple-effect powdery composite EM bacterial agent, the premixed solid fermentation culture medium and the simple expanding culture method of the multiple-effect powdery composite EM bacterial agent, efficient microorganisms are carefully selected, so that the microorganisms have a synergistic effect, and the purposes of environment protection, water quality improvement and green culturing are achieved.

Description

technical field [0001] The invention relates to the application of agricultural and environmental microorganisms in the breeding environment, in particular to a multi-effect powdery composite EM microbial agent which can be used for breeding, water quality improvement, soil improvement and environmental deodorization at the same time. Background technique [0002] There are many useful microorganisms in nature, and microorganisms are widely used in food, medicine and industry. Among the many microorganisms, we classify some of them as probiotics. This type of microorganism plays an important role in environmental protection, soil improvement, and maintaining the health of the host, so it is called EM (effective microorganism). [0003] EM bacteria include photosynthetic bacteria, lactic acid bacteria, yeast, actinomycetes, etc. It can improve the soil and metabolize in the soil to produce amino acids, organic acids, polysaccharides, vitamins, enzymes and other substances; ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12R1/07C12R1/01C12R1/125C12R1/10C12R1/25C12R1/23C12R1/225C12R1/865C12R1/72
CPCC12N1/16C12N1/20
Inventor 张梁石孔泉辛瑜李颜颜顾正华
Owner JIANGSU DAYOU BIOLOGICAL DEVAL