A kind of microbial preparation and its preparation method and application
A technology of microbial preparations and cow feed, applied in the field of microbial preparations and its preparation, to achieve the effects of broadening the source of microorganisms, good stability, and improving degradation rate
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[0018] In a specific embodiment, a preparation method of a microbial preparation is provided, the method comprising the following steps: inoculating Paenibacillus bovis CGMCC No.8333 in tomato juice sucrose medium for fermentation, and the fermented obtained after freeze-drying.
[0019] Further, in the above steps, the inoculum size of Paenibacillus bovis CGMCC No.8333 is preferably 2×10 7 ~1x10 8 cfu / mL; preferably 4x10 7 ~8x10 7 cfu / mL, preferably 6x10 7 cfu / mL.
[0020] Further, in the above steps, the tomato juice sucrose medium is prepared by the following method: ripe tomatoes are washed, peeled, squeezed, the resulting squeezed juice is filtered, boiled, centrifuged to take the supernatant, sucrose is added to heat and dissolve, and then cooled , adjust the pH value, cool after sterilization. Wherein the filtering method is preferably to use 80-120 mesh gauze to filter the juice, and the boiling time is preferably 1-10 minutes, preferably 3-7 minutes, more prefer...
Embodiment 1
[0035] 1. Materials and methods
[0036] (a) Preparation of seeds (fermentation strains):
[0037]Preparation of seeds (fermentation strains): dissolve the lyophilized powder of Paenibacillus CGMCC No.8333 (this bacterial strain has been disclosed in the Chinese patent with the publication number CN103740618A) with a small amount of sterile distilled water, and take a ring with an inoculation loop to mark Line in TYC solid medium (purchased from OXOID Co., UK), take out after 48 hours of aerobic culture at 30°C, pick a single colony with an inoculation loop and put it into 10mL TYC liquid medium (purchased from OXOID Co., UK), use vortex The shaker evenly dispersed the colony in the liquid medium, 30°C, 180rpm shaking culture for 24h, took it out, and inoculated it in TYC liquid medium (purchased from OXOID Co., UK) with a 2% (v / v) inoculum size, at 30°C After shaking at 180rpm for 24 hours, the culture was centrifuged at 15,000rpm for 10 minutes, the supernatant was discarde...
Embodiment 2
[0055] 1. Materials and methods
[0056] (a) preparation of seed (fermentation strain): with embodiment 1.
[0057] (b) Preparation of tomato juice sucrose medium: ripe tomatoes were cleaned, peeled, squeezed by a juicer, filtered through 80-mesh gauze to get the juice, boiled for 1 min, centrifuged at 12000 g for 8 min to get the supernatant, added 15% (w / v) sucrose and heated After dissolving, cool to room temperature, adjust the pH to 7.5 with food-grade alkali, sterilize at 125°C for 10 minutes, and cool to room temperature to obtain a sterile tomato juice sucrose medium.
[0058] (c) Determination method for the effect of microbial preparation on improving the degradation rate of dairy cow feed.
[0059] The addition ratio of microbial preparation is 0.1% (w / w), other is the same as embodiment 1.
[0060] 2. Preparation of microbial preparations and detection of its effect on improving the degradation rate of dairy cow feed
[0061] Paenibacillus bovis (Paenibacillus b...
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