A kind of probiotics and its preparation method and application
A microbial agent and utilization technology, applied in the field of microorganisms, to achieve good application prospects, broaden the source of microorganisms, and have the effects of good stability
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[0019] In a specific embodiment, a method of preparing a microdacterial agent, comprising the steps of: (a) inoculate the plant Lactobacillus CGMCC No. 0847, cheese Lactobacillus CGMCC No. 0828 inoculated in MRS medium, respectively. Fermentation is carried out, resulting in a fermentationery; (b) to precipitate the resulting fermentation liquid to the bacteria, and the bacterius is suspended, and the dry and dry mix is obtained.
[0020] Further, in the above steps (a), the amount of inoculation of the plant Lactobacillus CGMCC No. 0847, the inoculation of cheese Lactobacillus CGMCNO.0828 is preferably 7x10 6 ~ 3.5x10 7 CFU / ml; prefer 1.4x10 7 ~ 2.8x10 7 CFU / ML, better in 2.1x10 7 CFU / ml.
[0021] Further, in the above step (a), a preferred culture method is anaerobic culture.
[0022] Further, in the above step (a), a preferred fermentation temperature is 26 ° C to 42 ° C; preferably from 30 ° C to 38 ° C; more preferably 34 ° C.
[0023] Further, in the above step (a), ...
Embodiment 1
[0036] 1, material and method
[0037] (a) Preparation of seeds (fermented bacteria):
[0038] Plant Lactobacillus CGMCCN.0847: Phytobacillus CGMCC No. 0847 (this strain has been dissolved in a small amount of sterile distilled water in a small amount of sterile distilled water in the disclosure of the disclosure of CN 1467290A) Lines were removed from MRS solid medium (purchased from Oxoid Co., UK), at 37 ° C for anaerobic culture for 24 hours, the seeds were selected with a vaccinated ring into 10 mlmRs liquid medium (purchased from Oxoid Co., UK), using vortex The oscillator is uniformly dispersed in a liquid medium, and at an anaerobic culture at 37 ° C, it is taken out of the MRS liquid medium (purchased from oxoidco., UK), 37 ° C in the amount of anaerobic culture. After 24 h, the culture was centrifuged for 10 minutes. After discarding the supernatant, the bacteria was washed twice with a sterile distilled water, and the seed culture volume was suspended, and the seed fluid...
Embodiment 2
[0056] 1, material and method
[0057] The addition ratio of the microbial agent is 0.1% (w / w), and the other as in Example 1.
[0058] 2, preparation of microbial bacteria and its detection of the effect of milk feed utilization
[0059] Plant Lactobacillus CGMCC No. 0847, Cheese Lactobacillus CGMCC No. 0828 seeds were inoculated in a vaccinating amount of 1% (v / v) in the MRS liquid medium, 40 ° C anaerobic culture for 20 h, resulting in the fermentation liquid, respectively The two fermentation fluids were centrifuged for 10 min, discarded, taking a bacteria precipitated portion, hunging the bacterium with 10% (w / v) skim milk, and the plant Lactobacillus CGMCCN.0847, cheese Lactobacillus Cgmcc No. 0828 lyophilized powder is subjected to microbacterial bacteria B at 9: 1 (w / w), and is detected, wherein the number of live bacteria in plant Lactobacillus CGMCN NO.0847 is 6.3x10. 9 CFU / g, the number of live bacteria in Cheese Lactobacillus CGMCC No. 0828 is 0.7x10 9 CFU / ...
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