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SNP markers related to millet yardage traits and their detection primers and applications

A technology of millet and yardage is applied in the field of SNP markers and their detection primers, which can solve the problems of unreported research on the development and application of SNP molecular markers.

Active Publication Date: 2021-12-21
ZHANGJIAKOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on the QTL mapping and SNP markers related to the quantitative traits of millet is mainly focused on the development and utilization of SSR markers, but the development and application of SNP molecular markers using degenerate genome sequencing of population inbred lines to detect polymorphisms has not yet been reported.

Method used

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  • SNP markers related to millet yardage traits and their detection primers and applications
  • SNP markers related to millet yardage traits and their detection primers and applications
  • SNP markers related to millet yardage traits and their detection primers and applications

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1 High-throughput sequencing and SNP marker information analysis

[0034] In this example, the materials of the male parent "Ji Zhanggu No. 1" and the female parent "A2" of millet, the F1 generation materials of the two hybrids, and 441 materials of the F2 population of the 13th generation of selfing, that is, recombinant inbred lines (Recombinant inbred lines , abbreviated as RILs). Each material was planted in Zhangjiakou, Hebei, and the trait data such as yardage were recorded and sorted out. After the degenerate genome resequencing of each material, the alignment of the reference genome was completed, and the SNP marker was obtained. By analyzing the code number trait data, the associated SNP markers were obtained, and the SNP markers were verified by clonal sequencing.

[0035] In this example, the RADseq method was used to extract the genomic DNA of each sample individual, a total of 444 samples, including 441 RILs, 2 parents, and 1 F1 individual. The q...

Embodiment 2

[0058] Embodiment 2 SNP marker verification

[0059] According to the predicted bin marker and SNP site, compare the reference genome to obtain the gene sequence of the relevant region, select about 300 bp before and after the SNP site, design and develop SNP marker primers, and use the DNA of the paternal and maternal materials as templates for PCR amplification. Select the amplified products with normal primer amplification and the compounded predicted size of the PCR amplification products for gel cutting recovery and sequencing, and select the labeled primers with SNP site differences in the amplified gene sequences of the paternal and maternal materials. Using the F1 and F2 generation materials as templates, carry out PCR amplification of SNP marker primers and sequencing of the results, and select marker primers that are consistent with genotyping and phenotypic traits.

[0060] First, according to the sequencing results after PCR product recovery, the markers for the di...

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Abstract

The application discloses a SNP marker related to the trait of millet code number, detection primers and application thereof. The SNP marker related to the character of millet code number in the present application is located in the bin marker from the 27578869bp to the 28178402bp of the second chromosome, and the SNP marker is closely linked with the millet code number. The SNP markers related to the traits of millet yardage in this application can be used for molecular marker-assisted breeding of millet yardage traits. The detection of the SNP markers can predict the millet yardage, which provides a basis for early identification or screening breeding of millet yardage traits. The scientific basis has important theoretical and practical guiding significance for accelerating the genetic selection or improvement of millet varieties. This application detects the primer pair of SNP markers, which can type the code number of millet and detect the difference of SNP expression.

Description

technical field [0001] This application relates to the technical field of millet breeding, in particular to a SNP marker related to the trait of millet size and its detection primers and applications. Background technique [0002] my country is the origin of millet and the country with the largest cultivated area and the highest yield in the world, accounting for about 80% of the world's total. At the same time, my country is also the country with the largest number and diversity of millet genetic resources. [0003] Corn ears are composed of cobs and many grain yards. The yardage and grain number are important factors affecting the yield of millet. The study of the genes and their position functions related to the control of millet yardage, grain number and other yield traits is of great importance for guiding Millet genetics and breeding are of great significance. [0004] Single nucleotide polymorphism (singlenucleotide polymorphism, SNP) is a DNA sequence polymorphism ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵治海李双东王晓明张晓磊张耕耘史高雷魏玮张雅莉裴晶晶
Owner ZHANGJIAKOU ACAD OF AGRI SCI