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Construction method for chicken yingfen syndrome model

A construction method and model technology, applied in the direction of medical raw materials derived from bacteria, etc., to achieve scientific and accurate models, simple construction, and obvious clinical symptoms

Inactive Publication Date: 2018-10-16
POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similarly, there is no effective infection route or infection method that can be constructed for the stable poultry Yingfen syndrome pathogenesis model required for the development of traditional Chinese veterinary medicine.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1 Materials and methods:

[0017] 1.1 Poultry Escherichia coli virulent strain: E.coli c84008 strain, take 0.2mL of the preserved strain, inoculate it in 50mL of nutrient broth, culture it on a shaker at 37°C for 22h, and measure the concentration of the bacterial solution to 3×10 9 / mL.

[0018] 1.2 Experimental chickens: 60-day-old SPF chicks; number 100.

[0019] 1.3 Infection route and dose: 0.5 mL of E. coli fresh broth culture was injected intramuscularly into breast of each chicken.

[0020] 1.4 After the virus attack, observe the incidence every day.

[0021] 1.5 Judgment criteria Select the diseased chickens with the following disease characteristics at the same time, and it can be judged that the diseased chickens are manifested as Yingfen syndrome: (1) Obvious symptoms of depression such as feathers standing upright, shrinking head and closing eyes, loss of appetite, etc.; (2) ) Use a thermometer to measure the body temperature of each chicken, and the bod...

Embodiment 2

[0026] 1 Materials and methods:

[0027] 1.1 Poultry Escherichia coli virulent strain: E.coli c84008 strain, cultivated in nutrient broth for 24 hours at 37°C on a shaker, and the concentration of the bacterial solution was determined to reach 3×10 9 / mL.

[0028] 1.2 Experimental chickens: 40-day-old SPF chicks; number 200.

[0029] 1.3 Infection route and dosage: 0.3 mL of E. coli fresh broth culture was injected intramuscularly into breast of each chicken.

[0030] 1.4 After the virus attack, observe the incidence every day.

[0031] 1.5 Judgment criteria: the same as in Example 1.

[0032] 2 results:

[0033] After 24 hours of artificial infection of Escherichia coli, the symptoms of the infected chickens were obvious, such as high fever symptoms of listlessness, loss of appetite, and lethargy with eyes closed; the mucous membranes were red and crimson with cyanosis of cockscombs and meat whiskers; the skin and muscles were congested, purple-black, and internal organs ...

Embodiment 3

[0036] 1 Materials and methods:

[0037] 1.1 Poultry Escherichia coli virulent strain: E.coli c84008 strain, cultivated in nutrient broth at 37°C for 18 hours on a shaker.

[0038] 1.2 Experimental chickens: 35-day-old SPF male chicks; number 150.

[0039] 1.3 Infection route and dosage: 0.2 mL of Escherichia coli fresh broth culture was injected intramuscularly into breast of each chicken.

[0040] 1.4 After the virus attack, observe the incidence every day.

[0041] 1.5 Judgment criteria: the same as in Example 1.

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PUM

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Abstract

The invention relates to the technical field of traditional Chinese veterinary medicines, in particular to a construction method for a chicken construction-aspect pattern model. The construction method for the construction-aspect pattern model comprises the steps that SPF chickens of 30-60 days old are taken as experimental animals, and infection is carried out by adopting intramuscular injectionof avian escherichia coli virulent strains in chests. The construction method for the chicken construction-aspect pattern model creatively adopts a mode of intramuscular injection of the avian escherichia coli virulent strains and has the advantages of being capable of rapidly transmitting the pathogenic factors into the construction aspect, simple in construction, easy in operation, obvious in clinical symptoms, stable, reliable and suitable for traditional Chinese medicine prevention and treatment research on chicken yingfen patterns of traditional Chinese veterinary medicines.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine and veterinary medicine, in particular to a method for constructing a chicken camp syndrome model. Background technique [0002] Chinese herbal medicine is a treasure house of traditional medicine in my country. It has the characteristics of extensive functions, few side effects, less drug resistance, wide resources, low cost, and long-lasting effects. It is a treasure of the cultural heritage of traditional medicine in my country. medical potential. [0003] In poultry farming, the extensive use of antibiotics has led to serious bacterial resistance on the one hand, which directly affects the prevention and control of poultry diseases, and on the other hand causes antibiotic residues, which in turn affects human health. Traditional Chinese medicine has the characteristics of balancing yin and yang, eliminating evil and strengthening the body, and treating both symptoms and ro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74
Inventor 林树乾赵增成黄中利宋敏训李桂明傅剑杨世发
Owner POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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