Targeted area auxiliary support-based oocyte micromanipulation system and method

Abstract

The invention discloses a targeted area auxiliary support-based oocyte micromanipulation system and method. The micromanipulation system comprises a vacuum adsorption type suspension cell clamping device, an oocyte targeted area auxiliary support device and a piezoelectric ultrasonic oocyte micromanipulation device, wherein the vacuum adsorption type suspension cell clamping device comprises a rotating platform, a spiral electrode arranged on the rotating platform, and a vacuum pump arranged on the rotating platform, wherein an operation area for vacuum adsorption of oocytes is arranged in thecenter of the spiral electrode; the vacuum pump controls the operation area to perform vacuum adsorption on the oocytes; the oocyte targeted area auxiliary support device is used for providing an auxiliary support for a transparent zone of a targeted area of the oocytes; the piezoelectric ultrasonic oocyte micromanipulation device is used for performing specific micromanipulation on the oocytes after the auxiliary support is provided for the oocytes. According to the targeted area auxiliary support-based oocyte micromanipulation system disclosed by the invention, as the targeted area of the transparent zone of the oocytes has the auxiliary support, the transparent zone can be accurately located during puncturing; in combination with a microscopic vision technology, intracellular fixed-point operation can be really realized.

Description

technical field [0001] The invention relates to the technical field of biological micromanipulation, in particular to an oocyte micromanipulation system and method based on auxiliary support of targeted regions. Background technique [0002] Oocytes contain important biological genetic information and are the most important cells in the process of life breeding. Therefore, the micromanipulation technology of oocytes has always occupied the most important position in life science research and related medical technology. Micromanipulation techniques for different target sites at different development stages of oocytes, such as transgene injection technology, intracytoplasmic sperm injection (ICSI) technology, preimplantation genetic diagnosis (PGD) technology, somatic cell nuclear transfer technology, etc. emerge in endlessly. . [0003] The existing biological micromanipulation of oocyte is basically "one eye plus two arms (one holding arm and one operating arm)" mode. This...

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Problems solved by technology

Moreover, due to the image distortion caused by the large deformation of the cells, the position of the injection needle cannot be obtained by image processing technology in real time during the puncture process, and it can only be moved according to the predetermined position.

[0006] (2) Cell damage is large. During the traditional zona pellucida puncture process, because the cells are superelastic, the cells will be very deformed to achieve the puncture strength.

Most of the traditional oocyte micromanipulation modes focus on the damage to the zona pellucida during the puncture process, while ignoring the irreversible damage to the internal structure of the cell such as the cytoskeleton caused by high-intensity extrusion of the cell.

The piezoelectric microinjection technology without the constraint of the zona pellucida in the target area can indeed realize the micro-deformation puncture of oocytes in the traditional micromanipulation mode, but it is based on increasing the ultrasonic energy, and the cell damage has fallen into another a misunderstanding

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