Myocardial infarction and heart failure magnetic microparticle microfluidic biochip and detection method

A biochip and magnetic particle technology, applied in the detection of markers of myocardial infarction and heart failure in blood samples, in the field of magnetic particle microfluidic biochips, can solve the problems of inability to miniaturize and POCT equipment, reduce detection precision, and detect Sensitivity and specificity interference and other issues, to avoid missed detection and false negative phenomenon, shorten the diagnosis time, increase the effect of rescue time

Inactive Publication Date: 2018-10-16
微粒云科技(北京)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The first-generation detection technology competition method (radioimmunoassay, RIA): There are many factors affected, and the RIA sample requires a large amount. Because extraction is usually required before detection, it not only increases the operation steps, but also because the recovery rate of extraction is usually Only 80% to 90%, the coefficient of variation (CV) is relatively large, which reduces the precision of detection;
[0009] The second-generation detection method non-competitive method (radioimmunoassay, RIA): immunoassay using double-antibody sandwich, no extraction, high antibody specificity, its sensitivity, precision and specificity are better than competitive methods, but time-consuming Still long and difficult to apply to automation;
[0010] The third-generation detection method enzyme immunoassay, stationary phase immunochromatography: the double antibody plus core method immunoassay solid phase chroma

Method used

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  • Myocardial infarction and heart failure magnetic microparticle microfluidic biochip and detection method
  • Myocardial infarction and heart failure magnetic microparticle microfluidic biochip and detection method
  • Myocardial infarction and heart failure magnetic microparticle microfluidic biochip and detection method

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Effect test

Embodiment 1

[0091] The present invention is a magnetic particle microfluidic biochip for myocardial infarction and heart failure. The biosensor is pre-coated with three markers of myocardial infarction and heart failure (CTI / D-Dime / NT-proBNP). The first layer 30, the second layer 40, the microfluidic channel sealing layer 50 and the whole blood filter layer 60 are pasted on the microfluidic channel 11 of the PCB board 10 in sequence, thereby sealing the microfluidic channel 11, so that the reaction can be carried out under airtight conditions. To achieve the effect of micro-full laboratory. The first layer 30 is a biosensor airtight layer, containing the sampling hole 31 and the waste liquid hole 32; The pump guides the sample to be tested to flow in one direction from the mixing area 41 to the detection area (where the biosensor is located) and the waste liquid area 44; the microfluidic channel sealing layer 50 is the third layer to ensure that the sample flows in a closed pipeline, At ...

Embodiment 2

[0094] The preparation method of the three markers of myocardial infarction and heart failure (CTI / D-Dime / NT-proBNP) magnetic particle microfluidic biochip of the present invention:

[0095] Using the coupling method of superparamagnetic nano-magnetic particles, the superparamagnetic nano-magnetic particles include mixed magnetic beads with a diameter of 30nm and a diameter of 50nm. The reaction mechanism of the nano-magnetic particles is covalent bonding. The concentration of sodium acetate is reduced to 30nm and 50nm black colloidal superparamagnetic supersensitive magnetic particle mixed suspension, and the antibody / avidin with amino group is coupled. Quantitative detection reagent preparation steps are as follows,

[0096] 1. Preparation of 30nm+50nm superparamagnetic ultrasensitive magnetic particles:

[0097] (1) Preparation of 30nm magnetic beads: Take 0.75ml~2.25ml 4% Fe 3 o 4 Add the solution into 300ml of ultrapure water to make the final concentration of ferric o...

Embodiment 3

[0173] The specific steps of the detection of the three markers of myocardial infarction and heart failure (CTI / D-Dime / NT-proBNP) magnetic particle microfluidic biochip of the present invention:

[0174] (1) Take out the enterprise quality control product and the three markers of myocardial infarction and heart failure (CTI / D-Dime / NT-proBNP) magnetic particle microfluidic biochip quantitative detection kit from the refrigerator, and equilibrate to room temperature;

[0175] (2) Turn on the chip detector, perform system self-test and preheat for 5 minutes;

[0176] (3) Tear open the required aluminum foil bag containing the chip and take out the biochip, and mark the type and concentration of the quality control substance tested in the ID information position.

[0177] (4) Adding samples: Use a 100ul micropipette to respectively pipette 25ul of the internal control products of the three markers of myocardial infarction and heart failure into the sample loading area of ​​the cor...

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Abstract

The invention relates to a myocardial infarction and heart failure magnetic microparticle microfluidic biochip, which belongs to the technical field of POCT detection. The myocardial infraction and heart failure magnetic microparticle microfluidic biochip comprises a PCB board, wherein the PCB board is provided with a microflow passage, a biosensor is arranged in the microflow passage, the biosensor consists of a wafer platform and a point sample antibody covered on the wafer platform, the point sample antibody can form an immune complex with a magnetic bead coupling antibody and a marker protein, and the concentration of the marker protein can be judged by determining a magnetic resistance signal on the wafer platform. In the technical scheme, the magnetic bead has a larger specific surface area, and by combining more antibodies, the detection sensitivity is improved, and the specificity is higher than that of a fourth-generation chemical luminescent method. The invention also provides a detection method using the biochip. The heart markers such as myocardial infarction and heart failure can be simultaneously and quantitatively detected, the detection lower limit can be improved,and the leakage detection and false negative phenomenon can be effectively avoided.

Description

technical field [0001] The invention relates to a magnetic particle microfluidic biochip, which is mainly used for detecting markers of myocardial infarction and heart failure in blood samples; the invention also provides a detection method using the biochip, which belongs to the technical field of POCT detection. Background technique [0002] Cardiovascular disease is a serious disease that endangers human health and life, and has become the number one killer of human health in the 21st century. According to the "China Cardiovascular Disease Report 2015" and "China Cardiovascular Disease Report 2016" compiled by the National Center for Cardiovascular Diseases, the number of patients with cardiovascular diseases in my country is still growing rapidly. According to statistics, the number of patients with cardiovascular disease was 290 million in 2014, and this number has climbed to 290 million by 2015. According to the report estimates, about 3.5 million people die of cardio...

Claims

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Application Information

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IPC IPC(8): G01N33/68B01L3/00
CPCB01L3/5027B01L3/502746B01L2200/0668B01L2200/10B01L2300/0636B01L2300/0819B01L2400/043G01N33/6893G01N2800/324G01N2800/325
Inventor 张永勇
Owner 微粒云科技(北京)有限公司
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